Association between Characterizations of Bone Marrow Mesenchymal Stem Cells, Ejection Faction and Hospitalization Period in Patients with Severe Left Ventricular Dysfunction after Off-Pump Bypass Surgery

Background and Aim: Severe left ventricular dysfunction patients with ejection fraction ≤ 30% are at a higher risk for complications and mortality than patients with ejection fraction >30%. The death of cardio-myocytes at injured regions leads to myocardium dysfunction. Bone marrow-derived mesenchymal stem cells are undifferentiated cells that have been used for the regeneration of damaged cardio-myocytes. Due to the inherent capability of mesenchymal stem cells to improve cardiac functions, in this research, our objective was to explore the possible association of the mesenchymal stem cells proliferation rate with Coronary Artery Bypass Grafting outcomes in patients with severe left ventricular dysfunction after off-pump Coronary Artery Bypass Grafting. Methods: For investigating the possible association of mesenchymal stem cells proliferation with Coronary Artery Bypass Grafting outcomes (ejection fraction, hypertension risk, and the time of hospital stay), we collected bone marrow samples from 30 patients (18 men and 12 women) who underwent off-pump Coronary Artery Bypass Grafting at Afshar Hospital and Seyed Al-Shohada Hospital (Yazd, Iran). Mesenchymal stem cells were isolated and cultured; then, cells were counted after 4, 7, and 14 days using trypan-blue color, and doubling times were calculated. Results: There was an association between doubling time and ejection fraction after surgery. Ejection fraction in postoperative patients increased, but this association was not significant. Also, our study showed that the risk of hypertension is equal in male and female patients. There were no significant differences in mesenchymal stem cells doubling time with hypertension and the time of hospital stay in the ICU. Conclusion: Based on this study, we concluded that there was no significant relationship between the rate of mesenchymal stem cell proliferation and Coronary Artery Bypass Grafting outcomes in patients with severe left ventricular dysfunction. *Corresponding Author: Zakieh Sadat Sheikhalishahi; Email: [email protected] Please cite this article as: Esmailidehaj M, Mirhosseini SJ, Sheikhalishahi ZS. Association between Characterizations of Bone Marrow Mesenchymal Stem Cells, Ejection Faction and Hospitalization Period in Patients with Severe Left Ventricular Dysfunction after Off-Pump Bypass Surgery. Arch Med Lab Sci. 2019;5(4):30-36. https://doi.org/10.22037/amls.v5i4.3129

Association between Bone Marrow Mesenchymal Stem Cell Characterizations and the Administration of Antiarrhythmic Drugs in Patients with Severe Left Ventricular Dysfunction after Off-Pump Bypass Surgery

Background and Aim: Atrial fibrillation is the most common arrhythmia after coronary artery bypass grafting (CABG) and occurs in up to 30% of patients with heart failure. Mesenchymal stem cells (MSCs) can regenerate and improve cardiac function after tissue damage and are used in clinical trials. Due to the possible ability of MSCs to improve cardiac functions, in this work, we aimed to examine the probable association of the MSCs proliferation rate with the requirement for antiarrhythmic drugs in patients with severe left ventricular dysfunction after off-pump CABG. Methods: Bone marrow samples were obtained from the sternum of 30 patients who underwent off-pump CABG at Afshar Hospital and Seyed Al-Shohada Hospital. For calculating MSCs doubling time, the cells were counted after 4, 7, and 14 days using trypan-blue color and a hemocytometer. Results: There were no significant differences between MSCs' doubling time and the patient's age and gender. The percentage of women patients who require antiarrhythmic medicine was significantly higher than men after surgery. Also, we demonstrated that the BMSCs doubling time in female patients who received antiarrhythmic drugs was less than that of male patients who received antiarrhythmic drugs, but these differences were not significant. Conclusion: Based on this research, we concluded that women patients who received antiarrhythmic drugs were significantly higher than men, but there was no apparent relevancy between MSCs doubling time and antiarrhythmic drugs requirement in patients with severe left ventricular dysfunction. *Corresponding Author: Zakieh Sadat Sheikhalishahi; Email: [email protected] Please cite this article as: Esmailidehaj M, Mirhosseini SJ, Sheikhalishahi ZS. Association between Bone Marrow Mesenchymal Stem Cell Characterizations and the Administration of Antiarrhythmic Drugs in Patients with Severe Left Ventricular Dysfunction after Off-Pump Bypass Surgery. Arch Med Lab Sci. 2020;6:1-5 (e2). https://doi.org/10.22037/amls.v6.3129

Association between Bone Marrow Mesenchymal Stem Cell Characterization and Taking Inotropic Drugs in Patients with Severe Left Ventricular Dysfunction After Off-pump Bypass Surgery

Background and Aim: Left ventricular dysfunction is a frequent complication after coronary artery bypass graft (CABG) and is often treated with inotropic drugs to achieve suitable hemodynamic status. Bone marrow-derived mesenchymal stem cells (BMSCs) have potential effects on cardiac function. In this study, we aimed to identify the predictor role of MSCs in taking inotropic drugs in patients with severe left ventricular dysfunction undergoing CABG. Methods: The study included 30 patients who underwent off-pump CABG at Afshar Hospital and Seyed Al-Shohada Hospital. For investigating the possible association of BMSCs proliferation rate with taking inotropic drugs, the bone marrow samples aspired from patients' sternum during surgery. MSCs were isolated and counted after 4, 7, and 14 days using trypan-blue color, and then doubling times were calculated. Results: After cardiac surgery, the number of female patients who take inotropic drugs was significantly higher than men. Our data showed that the BMSCs doubling time in female patients who received inotropic drugs was less than that of male patients who received inotropic drugs (p < 0.05). Conclusion: Based on this investigation, we concluded that there was a clear relevance between the MSCs' doubling time and the inotropic drug requirements in patients who received inotropic drugs. *Corresponding Author: Zakieh Sadat Sheikhalishahi; Email: [email protected] Please cite this article as Esmailidehaj M, Mirhosseini S.J, Sheikhalishahi Z.S. Association Between Bone Marrow Mesenchymal Stem Cell Characterization and Taking Inotropic Drugs in Patients with Severe Left Ventricular Dysfunction After Off-pump Bypass Surgery. Arch Med Lab Sci. 2020;6:1-6 (e14). https://doi.org/10.22037/amls.v6.3129

Evaluation of 25-hydroxy Vitamin D Serum Levels and Thyroid-related Parameters in Patients with Type 2 Diabetes Mellitus and Healthy People in Shiraz, Iran

Background and Aim: The prevalence of type 2 diabetes mellitus (T2DM) is increasing globally. There is increasing evidence in the correlation between altered vitamin D levels, thyroid dysfunction, and T2DM. The goal of this study was to evaluate the association between serum 25-hydroxy vitamin D (25(OH) D), lipid profile, glucose and thyroid-related parameters among patients with T2DM and non-diabetic individuals. Methods: This case-control study was conducted on 228 individuals (110 type two diabetics and 118 healthy controls). The serum concentration of 25(OH) D was determined by chemiluminescence assay. Photometric methods measured serum levels of fasting blood glucose (FBG), calcium, phosphorous, total Cholesterol (TC), triacylglyceride (TG), high-density lipoprotein (HDL). Low-density lipoprotein (LDL) and VLDL levels were estimated from the Friedewald equation (LDL-C=TC–HDL-C–(TG/5)). The Elisa kit measured serum T4 and TSH. Results: 80% of Patients with T2DM and 71% of healthy individuals were suffering from vitamin D insufficiency. A non-significant relationship between TG (P=0.36), HDL (P=0.33), VLDL (P=0.36), T4 (P=0.56) calcium (P=0.39) and phosphorus (P=0.41) levels were showed in control and diabetic groups.  The levels of FBG (P=0.000), TC (P=0.001), LDL (P=0.004), TSH (P=0.000) were significantly higher, and the levels of 25 (OH) D (P=0.001) was significantly lower in the T2DM group compared to the non-diabetic group. Conclusion: We revealed that the serum level of 25 (OH) D is lower in patients with T2DM, and the TSH level is significantly higher compared to the non-diabetic group. Thyroid dysfunction and a low level of 25 (OH) D are associated with a high risk of T2DM. *Corresponding Author: Zakieh Sadat Sheikhalishahi; Email: [email protected] Please cite this article as: Rostami E, Najafi V, Behmard V, Panji M, Moravej FS, Dalvand S, Namdari A, Yavari N, Sheikhalishahi ZS. Evaluation of 25-hydroxy Vitamin D Serum Levels and Thyroid-related Parameters in Patients with Type 2 Diabetes Mellitus and Healthy People in Shiraz, Iran. Arch Med Lab Sci. 2020;6:1-6 (e22). https://doi.org/10.22037/amls.v6.3130

Relationship between Delivery Type and Jaundice Severity among Newborns Referred to Hospital

Background and Aim: Neonatal jaundice affects one of two newborns around the World and occurs when a baby has a high level of bilirubin in the blood. In recent decades, the cesarean section has increased, accompanied by higher neonatal jaundice risks compared with vaginal delivery. This study aimed to investigate the relationship between the jaundice severity and the delivery type and gender among newborns referred to a Qamar Bani Hashem Hospital in Khoy city (West Azerbaijan Province, Iran). Methods: In this cross-sectional study, we investigated randomly 309 newborns with asymptomatic jaundice, who referred to the hospital, from 2014 to 2018. The results of laboratory tests were recorded for all participating infants. Results: 49.19% of newborns with jaundice were born by cesarean section, and 49.19% were born by vaginal delivery. 141 (45.63%) of babies with jaundice were males, and 168 (54.37%) were females. Mean blood sugar (BS) (p=0.52), urea (p=0.48), creatinine(Cr) (p=0.69), Na (p=0.46), K (p=0.69), Ca (p=0.29), TB (p=0.58) and neonatal weight (p=0.14) within days 3 to 10 were not significantly different between vaginal delivery group and cesarean section group (p>0.05), while direct bilirubin showed significant variations (p<0.05). Mean serum direct bilirubin was 0.22±0.07 mg/dL in the normal vaginal delivery group and 0.25±0.09 mg/dL in the cesarean section group (p<0.05). Also, the level of K (P=0.04) was significantly higher in the male group compared to the female group. Conclusion: Based on our study, there is no significant relationship between the severity of jaundice and the type of delivery and gender in newborns. *Corresponding Author: Haleh Barmaki; Email: [email protected] Please cite this article as: Panji M, Behmard V, Varghaiyan Y, Sheikhalishahi ZS, Peyvasteh S, Seyedhashemi E, Zakeri M, Pooyanfar F, Barmaki H. Relationship between Delivery Type and Jaundice Severity among Newborns referred to Hospital. Arch Med Lab Sci. 2019;5(4):8-13. https://doi.org/10.22037/amls.v5i4.3214

A Comparative Study of 25-Hydroxyvitamin D Level and associated Parameters in CKD and Dialysis Patients in Shiraz, Iran

Background: Vitamin D deficiency and insufficiency generally observed in chronic kidney disease (CKD) and dialysis patients. The goal of this work was to compare the serum levels of vitamin D and its-related metabolic parameters in CKD patients and hemodialysis patients. Materials and Methods: A total of 109 dialysis patients and 99 CKD patients who had not consumed vitamin D for ≥ 1 year were enrolled in this study. 25-Hydroxy vitamin D (25-(OH) D) levels, calcium, alkaline phosphatase (ALP), hemoglobin (Hb), hematocrit (HCT), total iron-binding capacity (TIBC), parathyroid hormone (PTH) and phosphorous were evaluated in the serum of the patients. Results: 25-(OH) D levels in patients with CKD and dialysis patients were 31.73±13.34 ng/mL and 15.52±5.36 ng/mL, respectively. The levels of calcium (P=0.000), ALP (P=0.006), 25(OH) D (P=0.000), FBS (P=0.000), Hb (P=0.002), HCT (P=0.002) and TIBC (P=0.000) were significantly higher, and the levels of PTH (p=0.000) and phosphorous (P=0.000) were significantly lower in the CKD group compared to dialysis group. Conclusion: 25-(OH) D levels and its metabolic parameters except PTH and phosphorus are lower in patients on dialysis compared with the patients with CKD