Effect of replacement of fish meal with soybean meal plus supplementary enzymes on growth performance, survival rate and apparent digestibility of rainbow trout (Oncorhynchus mykiss)

This study was carried out to measure the effects of a supplementary multi enzyme on growth performance , survival rate and apparent protein digestibility of rainbow trout fed some diets containing different amounts of soy bean meal. Five exprimental diets with replacement of 25, 50, 75 and 100 percent of fish meal protein by soy bean meal protein were made and 0, 500 and 1000 ppm dosages of supplementary multi enzyme had used in each of them. By the means a diet with fish meal as the only source of protein has used as the control. So this study had 13 treatments. The trouts in 89.40±4.01 gr mean weight were stocked in 39 experimental fiberglass tanks in abundance of 30 fish per any tank. These specimens fed experimental diets for 8 weeks and ten of them in each tank fed same diets which added Cr2O3 to them for one more week to measure the apparent protein digestibility in them. The results shown that supplementary multi enzyme (Avizyme) which contains Protease , Amylase and Xylanase , caused increases in growth performance , survival rate and apparent protein digestibility in trouts which fed soybean meal. Also this study shown that using 1000 ppm of Avizyme in diets which containing soybean meal had the best results and the diet which contained 39 % soybean meal with this amount of enzymes, had no significant differences by the control in any of the studied factors

The effect of turmeric (Curcuma longa) on blood parameters and histopathological study of liver, kidney and intestine in juvenile Stellate sturgeon (Acipenser stellatus)

The aim of this study is to evaluate the Effect of different levels of turmeric on diet on Blood indicators and histopathological examination of liver, kidney and intestine in Juvenile Sevryuga. In this regard, 120 specimens were distributed in 12 Treatments (45 ± 0.5g) and (26 ± 0.5cm). The fish were fed in 4 groups with 4 experimental diets containing different levels of turmeric including: 0, 0.5, 1 and 2% formulated. At the end of the course , blood indices and histopathological examination of liver, kidney and intestine were evaluated. Increasing the dose of turmeric in 2% of the diet increased the amount of hematocrit, hemoglobin, RBC, AST and ALT and the difference between treatments was significant (P 0.05). The results of MCV ٬ MCH and MCHC analysis showed that the amount of the mentioned indicators decreased with increasing the dose of turmeric and the difference between the treatments was significant (

PVA/PANI/rGO ternary electrospun mats as metal-free anti-bacterial substrates

Successful performance of biocompatible hybrid systems in various biomedical applications such as wound healing patches, and scaffolds for stem cell preparation have been reported. However, relatively poor structural properties and further bacterial infection have been the major drawbacks for their commercialization. In order to improve the antimicrobial property of such structures, transition metals have been previously added to the media. However, the potential risk of metal pollution as well as hardship of processing has put this approach into obsolescence. Herein the ternary polyvinyl alcohol/reduced graphene oxide/polyaniline fibrous nanocomposites, as substitute for transition metal-containing nanocomposites, were prepared via electrospinning. The mats' structural properties (e.g. rheological, morphological, electrical and mechanical properties) and their antibacterial properties against E. coli bacteria cultures after two different treatments (including thermal and acid doping approaches) were systematically investigated. It was shown that in addition to significant structural improvement, an over 80% antibacterial property enhancement in treated mats in comparison to pristine PVA fibers were achieved. Finally the interaction and main effect analyses were used for suggesting the optimum antibacterial specimen conditions

Effects of vitamins E and Riboflavin (B2) and combinations of them on the hematological parameters of common carp, Cyprinus carpio L., fingerlings

In the present study, the effects of vitamins E, B2 and combinations of them on hematological parameters of common carp, Cyprinus carpio L., were investigated during a 56-day experiment. The various dietary levels of vitamins E, B2 and their combination were used as experimental treatments, as follows: T1: vitamin E (80 mg), T2: vitamin E (160 mg kg diet-1), T3: vitamin E (240 mg kg diet-1), T4: vitamin B2 (7 mg kg diet-1), T5: vitamin B2 (15 mg kg diet-1), T6: vitamin B2 (20 mg kg diet-1), T7: vitamin E (80 mg kg diet-1) + vitamin B2 (7 mg kg diet-1), T8: vitamin E (160 mg kg diet-1) + vitamin B2 (15 mg kg diet-1), T9: vitamin E (240 mg kg diet-1) + vitamin B2 (20 mg kg diet-1). One group not given vitamin supplements was the control. The values of red blood cells (RBC), hemoglobin (Hb), and hematocrit (Hct) were higher in T7 than in the other experimental treatments. The values of white blood cells (WBC) were higher in T2 than in the other treatments. The concentration of immunoglobulin (IgM) was also lower in T10 than in the other experimental treatments. The highest values of mean corpuscular hemoglobin (MCH) and mean corpuscular volume (MCV) were observed in T2. No significant differences were noted among experimental groups in the values of mean corpuscular hemoglobin concentration (MCHC). This study showed that vitamin E and B2 supplements alter the hematological parameters of common carp

The effect of isolated bacteria from the intestine of Beluga (Huso Huso) on some growth indices, amylase and lipase activity in body extract and carcass composition of the common carp (Cyprinus carpio) larvae

The aim of this study was to investigate the effect of probiotics isolated from the intestine of Beluga (Huso huso) fingerlings on growth factors, activity of digestive enzymes and carcass composition of common carp (Cyprinus carpio, Linnaeus 1758) larvae. Three hundred and sixty larvae with an average weight of approximately 200 mg after one-week adaptation to the new conditions, were fed with supplemented diets with blend of Bacillus subtilis, B. licheniformis and Saccharomyces cerevisiae in concentrations of 1.5 × 106 (T1), 3 × 106 (T2) and 4.5 × 106 (T3) CFU/100 g diet, respectively for 45 days. In control group, larvae were fed with diet without any supplementation. The experiment was carried out in a completely random design. At the end of the experiment, the results revealed that the highest final weight (35 ± 0.12 ­mg) and specific growth rate (0.94 ± 0.66%/day) were measured in T3. The highest feed conversion efficiency (46.52 ± 19.02%), protein efficiency ratio (0.37 ± 0.41), lipid efficiency ratio (1.22 ± 1.34), retained protein (0.0018 mg/day) and net protein utilization (0.0058%) were found in T3, while the lowest feed conversion ratio (2.34 ± 0.61) was obtained in T3. All of the above factors had significantly difference with control group (p 0.05). Amylase and lipase levels in the body extracts raised in the experimental treatments in comparison with the control group. However, only the amylase level displayed significant difference in comparison with control (p < 0.05). The experiment indicated that the blend of Bacillus and yeast at different concentrations displayed different effects on carcass analyses and feeding indices in this fish and the best results were obtained in T3 (4.5 × 106 CFU/100g diet)