The inviscid stability of supersonic flow past a sharp cone

The laminar boundary layer which forms on a sharp cone in a supersonic freestream, where lateral curvature plays a key role in the physics of the problem is considered. This flow is then analyzed from the point of view of linear, temporal, inviscid stability. The basic, non-axisymmetric disturbance equations are derived for general flows of this class, and a so called triply generalized inflexion condition is found for the existence of subsonic neutral modes of instability. This condition is analogous to the well-known generalized inflexion condition found in planar flows, although in the present case the condition depends on both axial and aximuthal wavenumbers. Extensive numerical results are presented for the stability problem at a freestream Mach number of 3.8, for a range of streamwise locations. These results reveal that a new mode of instability may occur, peculiar to flows of this type involving curvature. Additionally, asymptotic analyses valid close to the tip of the cone, far downstream of the cone are presented, and these give a partial (asymptotic) description of this additional mode of instability

Mapping chromatin conformation

Chromatin conformation capture (3C) and related techniques have become well-established methods to examine which distal DNA sequences are spatially located close to a locus of interest. Hi-C is a new addition to the growing set of 3C-based techniques that has extended the approach to a genome-wide analysis of nuclear chromatin using high-throughput DNA sequencing to generate global interaction maps for the entire genome

Absolute linear instability in laminar and turbulent gas/liquid two-layer channel flow

We study two-phase stratified flow where the bottom layer is a thin laminar liquid and the upper layer is a fully-developed gas flow. The gas flow can be laminar or turbulent. To determine the boundary between convective and absolute instability, we use Orr--Sommerfeld stability theory, and a combination of linear modal analysis and ray analysis. For turbulent gas flow, and for the density ratio r=1000, we find large regions of parameter space that produce absolute instability. These parameter regimes involve viscosity ratios of direct relevance to oil/gas flows. If, instead, the gas layer is laminar, absolute instability persists for the density ratio r=1000, although the convective/absolute stability boundary occurs at a viscosity ratio that is an order of magnitude smaller than in the turbulent case. Two further unstable temporal modes exist in both the laminar and the turbulent cases, one of which can exclude absolute instability. We compare our results with an experimentally-determined flow-regime map, and discuss the potential application of the present method to non-linear analyses.Comment: 33 pages, 20 figure

Carnivory in the Teasel Dipsacus fullonum — The Effect of Experimental Feeding on Growth and Seed Set

The teasel, Dipsacus fullonum is known to catch invertebrates in its water filled leaf bases, but experimental testing of reproductive benefits of this have been lacking. We report the effects of insect supplementation/removal and water removal during spring/summer on Dipsacus in two field populations. There were no significant treatment effects on biomass, but addition of dead dipteran larvae to leaf bases caused a 30% increase in seed set and the seed mass:biomass ratio. This study provides the first empirical evidence for reproductive benefit from carnivory in Dipsacus fullonum

Arabidopsis nucleolar protein database (AtNoPDB)

The Arabidopsis Nucleolar Protein Database (http://bioinf.scri.sari.ac.uk/cgi-bin/atnopdb/home) provides information on 217 proteins identified in a proteomic analysis of nucleoli isolated from Arabidopsis cell culture. The database is organized on the basis of the Arabidopsis gene identifier number. The information provided includes protein description, protein class, whether or not the plant protein has a homologue in the most recent human nucleolar proteome and the results of reciprocal BLAST analysis of the human proteome. In addition, for one-third of the 217 Arabidopsis nucleolar proteins, localization images are available from analysis of full-length cDNA–green fluorescent protein (GFP) fusions and the strength of signal in different parts of the cell—nucleolus, nucleolus-associated structures, nucleoplasm, nuclear bodies and extra-nuclear—is provided. For each protein, the most likely human and yeast orthologues, where identifiable through BLASTX analysis, are given with links to relevant information sources

The peri-urbanization of Europe:A systematic review of a multifaceted process

Urbanisation patterns in Europe since the 1950s have resulted in a swath of low-density discontinuous development, commonly called peri-urban areas. These areas are characterised by a mixed rural-urban character, are highly dynamic in nature, and are expected to continue growing rapidly in the next few decades. This paper presents a systematic review of the literature on changes in peri-urban areas in Europe. We analysed 142 cases from 121 studies that are spread across Europe, representing a wide range of peri-urban processes. Land cover changes were the most reported changes, followed by socioeconomic changes, land use changes, planning process changes, land management changes, and environmental changes. Over half of the cases reported co-occurring land-cover and socioeconomic processes of change. In addition, we analysed sequential and causal relations between these processes. In this analysis we found that peri-urbanization cannot be conceptualized as driver – land change – impact, because often relations between processes pointed in different directions. Therefore, we characterize peri-urbanization as a multifaceted process that can manifest itself differently in different case study areas. In addition, we found that planning precedes land change processes about as often as it follows these processes, illustrating the specific challenge for planners and policy makers in managing peri-urban areas

Ex vivo expansion of megakaryocyte precursors from umbilical cord blood CD34+ cells in a closed liquid culture system

AbstractUmbilical cord blood (UCB) provides a rich source of stem cells for transplantation after myeloablative therapy. One major disadvantage of UCB transplantation is delayed platelet engraftment. We propose to hasten platelet engraftment by expanding the number of megakaryocyte (MK) precursors (CD34/CD41 cells) through cytokine stimulation within a closed, pre-clinical liquid culture system. Clinical engraftment data suggest a 5- to 10-fold increase in MK precursors in a UCB unit can accelerate platelet engraftment, so this was our goal. Thirteen UCB samples from full-term births were Ficoll-separated and frozen for subsequent use. On thawing, the mononuclear cell population was positively selected for CD34+ expression. The cells were cultured in gas-permeable Teflon-coated bags in serum-free medium containing the following cytokines: recombinant human interleukin-3, recombinant human Flt3 ligand, recombinant human stem cell factor, and recombinant human thrombopoietin. MK lineage cell expansion was assessed using mononuclear cell count and flow cytometry (CD34/41, CD41, CD34/61, and CD61 expression) on days 7, 11, and 14. Optimal expansion of CD34/41 and CD41 cells was observed at day 11, with a median 6-fold and 33-fold increase in the starting cell doses, respectively. CD34/61 and CD61 cell expansion at day 11 was 7-fold and 14-fold, respectively. MK precursors can be successfully expanded from CD34+ UCB cells in a closed liquid culture system using interleukin-3, recombinant human Flt3 ligand, recombinant human stem cell factor, and recombinant human thrombopoietin to a level that should have a clinical impact in the transplantation setting. Our ex vivo expansion technique needs to be further optimized before it can be used in a pilot UCB transplantation trial. © 2003 American Society for Blood and Marrow TransplantationBiology of Blood and Marrow Transplantation 9:151-156 (2003