Needs of Green Buildings Fundamentals and its Implementation Flexibility for Prompt Effectiveness in Developing Countries

The construction concept is upgrading every year and major concern is given to the environment and human health. People’s activities for the sophistication has really degraded the component of environment which has impact in global warming, heat island effect and other hazardous effect causing negative impact to human health as well as environment. Now this trend need be changed and any construction work must be in collaboration with environment which is also coined as Green Building concept. In spite of many organizations’s effort this technology is being limited to some developed countries and no major progress is concluded in specially developing countries. Lack of awareness and misconception about the green building is limiting this concept. In order to make the green world very soon the system must be commenced from the government level itself. Government of every country should make grounds rules making basic green building categories and should register those building which have fulfilled the basic categories and certification must be given from government level itself evaluating the categories(Sustainable site development, Water saving, Energy efficiency, Material Selection, Indoor Environmental quality and Design) and innovation. Innovative design and implementation of engineering concept for every category must be given high priority. This paper forwards the concept of green buildings and basic categories which can be followed by government and basic planning design for everyone who can’t afford export which can be amend and implemented as per the context. Key words: Green Building, Environment, Awareness, Government, Engineering concept, Human healt

Effects of different rates of nitrogen and pinching on yield and yield attributes of African marigold (Tagetes erecta L.)

Proper pinching practice and the optimum rate of nitrogen (N) enhance the production of marigold. An experiment was conducted at a farmer’s field in Gadawa-4, Gangaparaspur, Dang, Nepal from July 2018 to November 2018 to investigate the effects of different rates of nitrogen and pinching on yield and yield attributes of African marigold (cv. Kolkata Local). Two factorial experiment was laid in the Randomized Complete Block Design (RCBD) with three replications. There were eight treatments, consist of four rates of nitrogen (0, 50, 100 and 150 kg/ha) and two levels of pinching (pinching and non-pinching). The maximum plant height (89.70 cm), the diameter of flower (4.29 cm) and the fresh weight per flower (4.32 g) and early days to 50% flowering (61.58) were obtained at non- pinching. The highest number of flowers (60.66), yield per plant (237.49 g) and yield per hectare (9.89 t/ha) were obtained with pinching. The highest plant height (92.20 cm) was recorded at 150 kg/ha of N but the highest yield per plant (238.18 g) and yield per ha (9.91 t/ha) was obtained at 50 kg/ha of nitrogen application. No significant effect was noted on the days to 50% flowering, number of flowers per plant, fresh weight per flower and diameter of flowers by different rates of nitrogen. The interaction of pinching and different rates of nitrogen showed non- significant effect on yield per plant and yield per ha. But, the combination of pinching and nitrogen rates at 50 kg/ha recorded the maximum yield per plant (249.20 g) and yield per ha (10.36 t/ha). Hence it is suggested to use pinching practice with optimum application of nitrogen @ 50 kg/ha to obtain high yield of marigold

RNA Interference Technology — Applications and Limitations

RNA interference (RNAi), an evolutionarily conserved mechanism triggered by double-stranded RNA (dsRNA), causes gene silencing in a sequence-specific manner. RNAi evolved naturally to mediate protection from both endogenous and exogenous pathogenic nucleic acids and to modulate gene expression. Multiple technological advancements and precision in gene targeting have allowed a plethora of potential applications, ranging from targeting infections in crop plants to improving health in human patients, which have been reviewed in this chapter

Aptamer-based multiplexed proteomic technology for biomarker discovery

Interrogation of the human proteome in a highly multiplexed and efficient manner remains a coveted and challenging goal in biology. We present a new aptamer-based proteomic technology for biomarker discovery capable of simultaneously measuring thousands of proteins from small sample volumes (15 [mu]L of serum or plasma). Our current assay allows us to measure ~800 proteins with very low limits of detection (1 pM average), 7 logs of overall dynamic range, and 5% average coefficient of variation. This technology is enabled by a new generation of aptamers that contain chemically modified nucleotides, which greatly expand the physicochemical diversity of the large randomized nucleic acid libraries from which the aptamers are selected. Proteins in complex matrices such as plasma are measured with a process that transforms a signature of protein concentrations into a corresponding DNA aptamer concentration signature, which is then quantified with a DNA microarray. In essence, our assay takes advantage of the dual nature of aptamers as both folded binding entities with defined shapes and unique sequences recognizable by specific hybridization probes. To demonstrate the utility of our proteomics biomarker discovery technology, we applied it to a clinical study of chronic kidney disease (CKD). We identified two well known CKD biomarkers as well as an additional 58 potential CKD biomarkers. These results demonstrate the potential utility of our technology to discover unique protein signatures characteristic of various disease states. More generally, we describe a versatile and powerful tool that allows large-scale comparison of proteome profiles among discrete populations. This unbiased and highly multiplexed search engine will enable the discovery of novel biomarkers in a manner that is unencumbered by our incomplete knowledge of biology, thereby helping to advance the next generation of evidence-based medicine

Role of gamma radiation in changing phytotoxic effect of elevated level of ozone in Trifolium alexandrinum L. (Clover)

AbstractThe present study was conducted on clover (Trifolium alexandrinum L. cv Wardan), to investigate the effect of ambient and elevated (ambient +10 ppb O3) ozone (O3) on plants grown in open top chambers (OTCs) germinated from gamma (γ) irradiated seeds. Dry seeds were subjected to irradiation with 0, 5, 10 and 20 krad doses of γ rays from 60Co source. Dose dependent differential responses were observed on growth and biomass, photosynthetic pigments, metabolites, antioxidative defense system of plant. Growth parameters and biomass of plants were severely affected under elevated O3 with increasing radiation doses, except, 5 krad which showed a reverse trend of response. Photosynthetic pigments and total soluble proteins were also reduced with higher dose of γ radiation and elevated O3. Reactive oxygen species formation and membrane damage increased significantly to different extents. Plants grown from seeds irradiated with low dose (5 krad) of γ irradiation depicted more induction of antioxidants (enzymatic and non–enzymatic) than higher doses suggesting their high ameliorative capability against elevated O3. Principal component analysis has also confirmed that plants grown from 5 krad γ irradiated seeds performed better against O3 depicting reduction in negative effect against elevated O3. The experimental findings evidently showed that 5 krad γ radiations altered the O3 induced stress and thus minimized the loss in biomass of the test plant

Modelling of Laser Scanning System to Determine its Associated Uncertainty of Measurement

In the fabrication of fine wires such as filaments of lamps and optical fibers, it is always necessary to measure the diameter in real-time and correct the extrusion process. For metrological purposes, laser beam scanning technique will produce similar results as comparative to diffraction-assisted methods. A computerized laser beam scanning measurement system (LSM) is designed and fabricated. The LSM consists of a low power helium-neon gas laser source, a specially designed collimating lens, a hysteresis synchronous motor, a photo detector and a protective replaceable window along with temperature sensor. The LSM is mathematically modeled to identify various error contributing sources. The LSM is calibrated using a set of national reference standard cylindrical pins and a set of national reference standard slots to determine its relative calibration factor. The uncertainty of measurement achieved in the range of (0-35) mm is ± (0.55 +39.2L/m) μm

Morphology of self-assembled structures formed by short peptides from the amyloidogenic protein tau depends on the solvent in which the peptides are dissolved

The aggregation behavior of peptides Ac-VQIVYK-amide (AcPHF6) and Ac-QIVYK-amide (AcPHF5) from the amyloidogenic protein tau was examined by atomic force microscopy (AFM) and fluorescence microscopy. Although AcPHF5 did not show enhancement of thioflavin T (ThT) fluorescence in aqueous buffer, distinct aggregates were discernible when peptide was dissolved in organic solvents such as methanol (MeOH), trifluoroethanol (TFE), and hexafluoroisopropanol (HFIP) dried on mica and examined by AFM. Self-association was evident even though the peptide did not have the propensity to form secondary structures in the organic solvents. In dried films, the peptide adopts predominantly β-conformation which results in the formation of distinct aggregates. ThT fluorescence spectra and fluorescence images indicate the formation of fibrils when AcPHF6 solutions in organic solvents were diluted into buffer. AcPHF6 had the ability to organize into fibrillar structures when AFM samples were prepared from peptide dissolved in MeOH, TFE, HFIP, and also when diluted into buffer. AcPHF6 showed propensity for β-structure in aqueous buffer. In MeOH and TFE, AcPHF6 showed helical and β-structure. Morphology of the fibrils was dependent on peptide conformation in the organic solvents. The structures observed for AcPHF6 are formed rapidly and long incubation periods in the solvents are not necessary. The structures with varying morphologies observed for AcPHF5 and AcPHF6 appear to be mediated by surfaces such as mica and the organic solvents used for dissolution of the peptides

Organic solvent mediated self-association of an amyloid forming peptide from &#946;<SUB>2</SUB>-microglobulin: an atomic force microscopy study

Human &#946;2-microglobulin (&#946;2m) forms amyloid fibrils in hemodialysis related amyloidosis. Peptides spanning the &#946; strands of &#946;2m have been shown to form amyloid fibrils in isolation. We have studied the self-association of a 13-residue peptide Ac-DWSFYLLYYTEFT-am (P&#946;2m) spanning one of the &#946;-strands of human &#946;2-microglobulin when dissolved in various organic solvents such as methanol (MeOH), trifluoroethanol (TFE), hexafluoroisopropanol (HFIP), and dimethylsulfoxide. We have observed that P&#946;2m forms amyloid fibrils when diluted from organic solvents into aqueous buffer at pH 7.0 as judged by increase in thioflavin T fluorescence. Fibril formation was observed to depend on the solvents in which peptide stock solutions were prepared. Circular dichroism spectra indicated propensity for helical conformation in MeOH, TFE, and HFIP. In buffer, &#946;-structure was observed irrespective of the solvent in which the peptide stock solutions were prepared. Atomic force microscopy images obtained by drying the peptide on mica from organic solvents indicated the ability of P&#946;2m to self-associate to form nonfibrillar structures. Morphology of the structures was dependent on the solvent in which the peptide was dissolved. Peptides that have the ability to self-associate such as amyloid-forming peptides would be attractive candidates for the generation of self-assembled structures with varying morphologies by appropriate choice of surfaces and solvents for dissolution

Combining traditional embryology lectures with technology and perception of students toward it

Background: Technology-enhanced learning is reliable, established, and effective teaching tool for better understanding of the subject. This study addresses an important issue in medical education, namely, the enhancement of the widely criticized lecture style of teaching by engaging students more actively during class through the use of videos or animations. This study describes the development, implementation, and evaluation of a traditional anatomy lecture series combined with technology. Aim and Objectives: The aim of the study is (a) to combine three-dimensional (3D) videos and animations with traditional embryology lectures and (b) to evaluate the 1st-year MBBS students' perception about this method of learning of embryology. Methodology: This qualitative study was conducted in the Department of Anatomy at Guru Gobind Singh Medical College, Faridkot, during the theory classes of embryology over a period of approximately 3 months among 100 1st-year MBBS students. The students were shown 3D videos and animations of embryology during traditional didactic lecture to explain all the aspects of the development of different systems of the body in 25 classes, of 1-h duration each. Feedback was taken using a structured questionnaire to know the perception of students toward this exercise of using technology during lecture of embryology. Results: A total of 100 MBBS first professional students took part in the study and submitted questionnaire. The students were satisfied with this method of technology-enhanced learning and described better understanding of the concepts with improved learning. More than 90% of the students found that combining 3D videos and animations with traditional lecture helped in solving the confusion and misconceptions regarding topic and did make the topic more interesting. They further added that they wanted to have such type of technology (videos)-enhanced lectures in all other topics of anatomy and in other subjects as well. Conclusions: Technology used in the form of 3D videos and animations changed their attitude toward learning and their learning was improved. It provided satisfaction to students in terms of better learning and understanding of the subject