Interaction effect of seedling age and different doses of GA3 on growth and yield of tomato (Lycopersicon esculentum Mill.)

An experiment was conducted in the Horticultural Farm of Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka-1207 during the period from October 2010 to March 2011 to find out the effect of GA3 and seedling age on the growth and yield of tomato. The experiment was laid out in randomized complete block design (RCBD) with three replications. The experiment consisted of four concentration of GA3 such as control G0 = no GA3, G1= 75 ppm GA3, G2 = 100 ppm GA3 and G3 =125 ppm GA3; three different seedling ages such as S1= 20 days, S2 = 25 days and S3 = 30 days old seedling. All parameter varied significantly at different concentration of GA3 in different DAT. The combined effect of G3S3 (125 ppm GA3 with 30 days old seedling) gave the maximum yield (90.28 t ha-1), on the other hand combined effect of G0S1 (no GA3 with 20 days old seedling) gave the minimum yield (59.83 t ha-1). The maximum yield (2.79 kg) plant-1 was recorded from treatment combination of G3S3 and the minimum yield (1.20 kg) was recorded from treatment combination G0S1. The maximum yield (32.47 kg) plot-1 was recorded from treatment combination G3S3 and the minimum yield (21.52 kg) plot-1 was recorded from treatment combination G0S1. The maximum yield (90.28 t ha-1) was recorded from treatment combination of G3S3 and the minimum yield (59.83 t ha-1) was recorded from treatment combination of G0S1. Therefore, 125 ppm GA3 with 30 days old seedling was found suitable combination for tomato production

Current Status of Trace Metal Pollution in Soils Affected by Industrial Activities

There is a growing public concern over the potential accumulation of heavy metals in soil, owing to rapid industrial development. In an effort to describe the status of the pollutions of soil by industrial activities, relevant data sets reported by many studies were surveyed and reviewed. The results of our analysis indicate that soils were polluted most significantly by metals such as lead, zinc, copper, and cadmium. If the dominant species are evaluated by the highest mean concentration observed for different industry types, the results were grouped into Pb, Zn, Ni, Cu, Fe, and As in smelting and metal production industries, Mn and Cd in the textile industry, and Cr in the leather industry. In most cases, metal levels in the studied areas were found to exceed the common regulation guideline levels enforced by many countries. The geoaccumulation index (Igeo), calculated to estimate the enrichment of metal concentrations in soil, showed that the level of metal pollution in most surveyed areas is significant, especially for Pb and Cd. It is thus important to keep systematic and continuous monitoring of heavy metals and their derivatives to manage and suppress such pollution

Epigenetic Regulation of MicroRNA Genes and the Role of miR-34b in Cell Invasion and Motility in Human Melanoma

Invasive melanoma is the most lethal form of skin cancer. The treatment of melanoma-derived cell lines with 5-aza-2\u27-deoxycytidine (5-Aza-dC) markedly increases the expression of several miRNAs, suggesting that the miRNA-encoding genes might be epigenetically regulated, either directly or indirectly, by DNA methylation. We have identified a group of epigenetically regulated miRNA genes in melanoma cells, and have confirmed that the upstream CpG island sequences of several such miRNA genes are hypermethylated in cell lines derived from different stages of melanoma, but not in melanocytes and keratinocytes. We used direct DNA bisulfite and immunoprecipitated DNA (Methyl-DIP) to identify changes in CpG island methylation in distinct melanoma patient samples classified as primary in situ, regional metastatic, and distant metastatic. Two melanoma cell lines (WM1552C and A375 derived from stage 3 and stage 4 human melanoma, respectively) were engineered to ectopically express one of the epigenetically modified miRNA: miR-34b. Expression of miR-34b reduced cell invasion and motility rates of both WM1552C and A375, suggesting that the enhanced cell invasiveness and motility observed in metastatic melanoma cells may be related to their reduced expression of miR-34b. Total RNA isolated from control or miR-34b-expressing WM1552C cells was subjected to deep sequencing to identify gene networks around miR-34b. We identified network modules that are potentially regulated by miR-34b, and which suggest a mechanism for the role of miR-34b in regulating normal cell motility and cytokinesis

RA-MAP, molecular immunological landscapes in early rheumatoid arthritis and healthy vaccine recipients

Rheumatoid arthritis (RA) is a chronic inflammatory disorder with poorly defined aetiology characterised by synovial inflammation with variable disease severity and drug responsiveness. To investigate the peripheral blood immune cell landscape of early, drug naive RA, we performed comprehensive clinical and molecular profiling of 267 RA patients and 52 healthy vaccine recipients for up to 18 months to establish a high quality sample biobank including plasma, serum, peripheral blood cells, urine, genomic DNA, RNA from whole blood, lymphocyte and monocyte subsets. We have performed extensive multi-omic immune phenotyping, including genomic, metabolomic, proteomic, transcriptomic and autoantibody profiling. We anticipate that these detailed clinical and molecular data will serve as a fundamental resource offering insights into immune-mediated disease pathogenesis, progression and therapeutic response, ultimately contributing to the development and application of targeted therapies for RA.</p

Comparative analysis of ABO-incompatible kidney transplantation with ABO-compatible transplantation: A single-center experience from Eastern India

This study aimed to compare the outcomes of ABO-incompatible kidney transplantation (ABOiKT) with ABO-compatible kidney transplantation (ABOcKT) in a singlecenter study. A total of 30 consecutive ABOiKT recipients (ABOiKTR) from April 2014 to June 2015 were included in this study. All the patients received rituximab 200 mg/body for B-cell depletion. Plasmapheresis was done for anti-ABO antibody removal. The target anti-ABO titer was kept at <1:8. The outcomes of this group of patients were compared with that of thirty ABOcKT recipients. Both the groups received similar induction therapy with antithymocyte globulin and methylprednisolone. After a follow-up period of one year, the outcomes of both the groups were compared in terms of patient survival, graft survival, graft function, incidence of rejections, infective complications, and duration of posttransplant hospital stay. The patient survival in both the groups of patients was 96.67%. The death-censored graft survival was 96.67% in both the groups. The average serum creatinine level, estimated glomerular filtration rate, incidence of rejections, infective episodes, and posttransplant hospital stay were comparable in both the groups. The outcomes of ABOiKT were comparable with ABOcKT and as such, this modality can expand the living donor pool substantially

Antimicrobial potentiality of a phenothiazine group of antipsychotic drug -prochlorperazine

828-830The antipsychotic drug, prochlorperazine (Pcp), was tested for its antimicrobial efficacy against 103 strains belonging to both gram positive and gram negative bacteria. The drug was found to possess maximum activity against Staphylococcus aureus, Vibrio cholerae and Shigella spp. Pcp was moderately active against E. coli but most of the strains belonging to Bacillus spp, Klebsiella spp, Salmonella spp and Lactobacillus spp were found to be resistant to this drug. The drug was tested for its mode of anti bacterial activity against Shigella dysenteriae 1 and it was found to be bacteriostatic in action. In in vivo studies, Pcp offered significant protection to Swiss albino mice at concentrations of 0.75 μg/g (P<0.01) and 1.5 μg/g (P) body weight when challenged with 50 median lethal dose of Salmonella typhimurium NCTC 74. Thus the result depicts that prochlorperazine may emerge as a strong antimicrobial drug to replace the conventional antibiotics and to overcome the problem of drug resistance.</span

3-(4-Chlorobutyl)-3H-indole-5-carbonitrile. Corrigendum

In the paper by Manikandan et al. [IUCrData (2017). 2, x170376], a proton on the indole N was missed in the structure solution. The proton is now added and the changes are reported here. The title, scheme, Figs. 1 and 2, the hydrogen-bonding table, the crystallographic data table and description of the supramolecular architectures are corrected

3-(4-Chlorobutyl)-3H-indole-5-carbonitrile

In the title compound, C13H12ClN2, the indole moiety and the chloroalkyl substituent are nearly coplanar, making a dihedral angle of 1.27 (10)°. In the crystal, a supramolecular sheet parallel to the ab plane is generated via weak C—H...Cl hydrogen bonds, aromatic π–π stacking [centroid–centroid distances of 3.5563 (13) and 3.6792 (13) Å] and C—H...π interactions