Energy-efficient building management via model predictive control

The growing world population and energy consumption, along with the depletion of fossil energy resources and increasing concern regarding the pollution of the environment, make the world-energy problem the largest challenge for technology in the forthcoming decades [1]. Distributed generation could play a major role in the technological changes of the new electricity service paradigm. The use of localized energy sources is only one of the faces of energy management in buildings. A large part of the produced energy is used for heating and cooling systems, in order to maintain acceptable levels of comfort for the occupants of the apartments (hereafter referred to as "users"). Therefore, at a local level, there is a need for the definition and the testing of intelligent algorithms that can automatically manage distributed energy sources, at the same time taking into account the management of heating/ cooling systems

Energy-efficient building management via model predictive control

The growing world population and energy consumption, along with the depletion of fossil energy resources and increasing concern regarding the pollution of the environment, make the world-energy problem the largest challenge for technology in the forthcoming decades [1]. Distributed generation could play a major role in the technological changes of the new electricity service paradigm. The use of localized energy sources is only one of the faces of energy management in buildings. A large part of the produced energy is used for heating and cooling systems, in order to maintain acceptable levels of comfort for the occupants of the apartments (hereafter referred to as "users"). Therefore, at a local level, there is a need for the definition and the testing of intelligent algorithms that can automatically manage distributed energy sources, at the same time taking into account the management of heating/ cooling systems

Physiological roles of histidine acid phytase from Pantoea sp. 3.5.1.

Microbial phytases represent a potential alternative way to produce myo-inositol phosphate isomers with therapeutic properties, whereas the chemical synthesis of these compounds is inefficient and costly. The aim of this work is to study the physiological role of histidine acid phytase in Pantoea sp 3.5.1 cells. Promoter region of the phytase gene was studied and potential binding sites for transcription factors RpoD15 and MetJ were identified by the methods of bioinformatic analysis. Genomic locus carrying the phytase gene was characterised and compared with the same genomic loci of other enterobacteria. It was found that the isolated and purified phytase from Pantoea sp. 3.5.1 refers to the Agp- group of histidine acid phytases and has dual physiological role in the bacterial cell. As glucose-1-phosphatase it is involved in glycolysis processes. The data obtained by stereospecifity analysis helped to identify that phytase from Pantoea sp. 3.5.1 is the enzyme that carries out phytate hydrolysis by the second path and forms D/L-myo-inositol-1,2,4,5,6-pentakisphosphate as the end product

Cloning of Phytase Genes from Pantoea Sp. 3.5.1 and Bacillus ginsengihumi M2.11 in Pichia pastoris

© 2018, Springer Science+Business Media, LLC, part of Springer Nature. Phytases (myo-inositol hexakisphosphate hydrolase) catalyze the hydrolysis of phytate to inorganic phosphate and less phosphorylated myo-inositol derivatives and are widely used as feed additives in animal nutrition. Nevertheless, nowadays, there is a constant search for new phytases and new expression systems for better production of these important enzymes. In this study, we report cloning of two novel bacterial phytases belonging to the different enzyme classes and having different properties in the methylotrophic yeast Pichia pastoris. Sequences of agpP and phyC genes, encoding histidine acid phytase from Pantoea sp. 3.5.1 and β-propeller phytase from Bacillus ginsengihumi M2.11, respectively, were optimized and chemically synthesized according to the P. pastoris codon usage bias. The optimized genes were cloned into the yeast vectors pPINK-HC and pPINK-LC under the control of the inducible promoter AOX1 and two different signal peptides—signal sequence of α-amylase gene from Aspergillus niger and presequence of inulinase gene from Kluyveromyces maxianus. PCR analysis, restriction analysis, and DNA sequencing confirmed correct integration of agpP and phyC genes into P. pastoris genome. As a result, recombinant strains of P. pastoris with codon-optimized bacterial phytase genes (agpP and phyC), encoding histidine acid and β-propeller phytases, integrated into the genome under the alcohol oxidase promoter AOX1 and two different signal peptides, were obtained. Recombinant phytase AgpP was stably expressed and secreted into the culture medium of yeasts, whereas the expression of phyC gene was only confirmed at transcription level

Risk management of an education project

© 2016 by iSER, International Society of Educational Research. The urgency of the problem stated in the article is stipulated by the fact that the organizational and methodical conditions of effective education projects risk management being adequate to the current stage of the society development are not developed perfectly. It is related to the fact that the circumstances actualizing the concept of "risk" in the modern educational environment is as a rule neglected and instead of it is used only its economic component, which results in effectiveness reduction. The purpose of the present article is the identification of the circumstances, actualizing the concept of "risk" in the modern educational environment and formulation of organizational and methodological conditions for effective risk management of educational projects. The leading approach to the study of this problem is systematization that allows to consider the problem as a whole system necessary for effective risk management of educational projects. The present article identifies the circumstances actualizing study of the "risk" phenomenon in the modern educational environment, formulates the basic contradiction in the study of the risks of educational projects and formulates organizational and methodical conditions of effective risk management of education projects. Organizational and methodical conditions of the effective risk management of educational projects are designated for creating a more efficient models and practices of risk management of the educational project. They may be useful in the sphere of educational employees who design, plan, implement, monitor and evaluate educational projects of different levels

The Genetic Mechanism of Resistance to Antibiotics in Bacillus pumilus 3-19 Strain

© 2016, Springer Science+Business Media New York.Treatment of bacterial infections becomes increasingly complicated due to the emergence of bacterial resistance to antimicrobial agents. Until now, most studies on bacterial antibiotic resistance have focused mainly on clinically relevant isolates pathogenic microorganisms and lactic acid bacteria. Very limited information on the antimicrobial susceptibility profiles of Bacillus spp. is available. In this paper, we used Bacillus pumilus 3-19 strain, a derivative of a wild B. pumilus 7P strain that has acquired resistance to streptomycin. Comparative analysis of genomes showed that B. pumilus 3-19 became resistant to streptomycine due to a mutation in 56 codon of the rpsL gene (S12 protein of 30S ribosomal subunit) that resulted in the replacement of lysine with asparagine in the binding site of streptomycin. Bioinformatic analysis of rpoB gene (β-subunit of RNA polymerase) showed that there is also a point mutation in 185 codon, which can lead to rifampicin resistance. Nevertheless, B. pumilus 3-19 strain remained sensitive to rifampicin in disc diffusion assay

Improving the quality of professional training of students in the distance learning

© 2017 Serials Publications.The relevance of the problem under investigation is due to the fact that distance education in mass, especially in a professional higher school, is of particular importance in terms of fundamental changes in the needs of society in a variety of professions and requirements imposed on graduates of modern educational institutions. This form of training together with the expansion of information technologies can effectively solve the problem of training students and life-long learning throughout a person's life. It should be noted that it follows the logic of the development of the education system and society as a whole and can act as a tool not only to meet, but also to form educational needs of a person, in accordance with the interests of society and the state. Distance education broadens the mind of the person giving the sense of belonging to the world of humanistic ideas and the information society, creating a sense of responsibility, is able to solve the problem of teacher training more than any other method of education. In the same context, the opportunities of distance education to develop verbal and cogitative activities and information culture are considered. The promising option for training future teachers for the work in the "person-toperson" (face-to-face) system is the network of educational institutions that offer distance learning courses. Under the functioning of this system the integrity of content and methodological training environments of geographically disparate educational institutions is ensured. As a result of the use of network principles the integrity of educational institutions can be effectively guaranteed and realized in continuity of principles and approaches to the management of different functional and territorial subsystems, and in the more distant future - its equitable integration into the world community

Huntington’s disease modeling on HEK293 cell line

Huntington’s disease is a hereditary neurodegenerative disorder caused by CAG trinucleotide repeat expansion in the first exon of HTT gene. The mutant HTT protein has an elongated polyglutamine tract and forms aggregates in the nuclei and cytoplasm of the striatal neurons. The pathological processes occurring in the medium spiny neurons of Huntington’s disease patients lead to neurodegeneration and consequently to the death. The molecular mechanisms of the pathology development are difficult to study due to the limited material availability and late onset of the manifestation. Therefore, one of the important tasks is generation of an in vitro model system of Huntington’s disease based on human cell cultures. The new genome editing approaches, such as CRISPR/Cas9, allow us to generate isogenic cell lines that can be useful for drug screening and studying mechanisms of molecular and cellular events triggered by certain mutation on an equal genetic background. Here, we investigated the viability and proliferative rate of several mutant HEK293 cell clones with mutations in the first exon of HTT gene. The mutant clones were obtained earlier using CRISPR/Cas9 genome editing technology. We showed that mutant cells partially reproduce the pathological phenotype, that is, they have reduced proliferation activity, an increased level of apoptosis and high sensitivity to treatment with 5μM MG132 proteasome inhibitor compared to the original HEK293 Phoenix cell line. Our results indicate that the mutation in the first exon of HTT gene affects not only neurons, but also other types of cells, and HEK293 cell clones bearing the mutation can serve as in vitro model for studying some mechanisms of HTT functioning

Cloning and Heterologous Expression of Phytase Gene from Pantoea sp. 3.5.1

© 2016, Springer Science+Business Media New York.Phytases (myo-inositol hexakisphosphate phosphohydrolase) catalyzes the stepwise hydrolysis of phosphate groups from phytic acid (myo-inositol hexakisphosphate) or its salt phytate. These enzymes could be potentially used for the stereospecific and efficient production of different myo-inositol phosphate isomers with therapeutic features. In the present study, we cloned the 1728 bp open reading frame encoding Pantoea sp. 3.5.1 phytase into the expression vector pET28a. The recombinant Escherichia coli BL21 pLysS pET28a/agpP strain expressing Pantoea sp. 3.5.1 AgpP phytase was obtained