El Diario de Pontevedra : periódico liberal: Ano XXVII Número 7864 - 1910 xullo 27

Figure S1. Protein expression analysis to aid in the molecular classification of breast cancer cell lines. Cell lysates containing equivalent amounts of total cell protein from the indicated non-tumorigenic breast (184B5, MCF10A, MCF10F, MCF12A) and breast cancer cell lines were probed with the indicated antibodies and GAPDH (loading control). The amount of total protein loaded per lane for each set of blots was as follows: ER (50 μg), PR (50 μg; PR-A is 81 kDa and PR-B is 116 kDa), HER2 (10 μg), EGFR (50 μg), p53 (50 μg), and GAPDH (50 μg). The molecular weight of each protein is indicated. (PDF 259 kb