A Sensor-Based Visual Effect Evaluation of Chevron Alignment Signs’ Colors on Drivers through the Curves in Snow and Ice Environment

The ability to quantitatively evaluate the visual feedback of drivers has been considered as the primary research for reducing crashes in snow and ice environments. Different colored Chevron alignment signs cause diverse visual effect. However, the effect of Chevrons on visual feedback and on the driving reaction while navigating curves in SI environments has not been adequately evaluated. The objective of this study is twofold: (1) an effective and long-term experiment was designed and developed to test the effect of colored Chevrons on drivers’ vision and vehicle speed; (2) a new quantitative effect evaluation model is employed to measure the effect of different colors of the Chevrons. Fixation duration and pupil size were used to describe the driver’s visual response, and Cohen’s d was used to evaluate the colors’ psychological effect on drivers. The results showed the following: (1) after choosing the proper color for Chevrons, drivers reduced the speed of the vehicle while approaching the curves. (2) It was easier for drivers to identify the road alignment after setting the Chevrons. (3) Cohen’s d related to different colors of Chevrons have different effect sizes. The conclusions provide evident references for freeway warning products and the design of intelligent vehicles

Distinct miRNAs associated with various clinical presentations of SARS-CoV-2 infection.

MicroRNAs (miRNAs) have been shown to play important roles in viral infections, but their associations with SARS-CoV-2 infection remain poorly understood. Here, we detected 85 differentially expressed miRNAs (DE-miRNAs) from 2,336 known and 361 novel miRNAs that were identified in 233 plasma samples from 61 healthy controls and 116 patients with COVID-19 using the high-throughput sequencing and computational analysis. These DE-miRNAs were associated with SASR-CoV-2 infection, disease severity, and viral persistence in the patients with COVID-19, respectively. Gene ontology and KEGG pathway analyses of the DE-miRNAs revealed their connections to viral infections, immune responses, and lung diseases. Finally, we established a machine learning model using the DE-miRNAs between various groups for classification of COVID-19 cases with different clinical presentations. Our findings may help understand the contribution of miRNAs to the pathogenesis of COVID-19 and identify potential biomarkers and molecular targets for diagnosis and treatment of SARS-CoV-2 infection

The Source of Tax Law in the PRC: Legislative Organs and Their Legal Documents

In the comparison of the source of tax law between Australia and China, it is difficult for the public to understand each law and regulatory documents being proposed in making reasonable decisions via their interpretations in China. This paper would introduce the tax administrative power of the ATO and the tax advice system in Australia, especially the ruling system. This paper finds out that it is both an urgent and highly beneficial affair to build an advice framework such as the public rulings in Australia in China.

Suppression of Cancer Cell Stemness and Drug Resistance via MYC Destabilization by Deubiquitinase USP45 Inhibition with a Natural Small Molecule

Cancer stem cells (CSCs) play significant roles in cancer development, drug resistance and cancer recurrence. In cancer treatments based on the CSC characteristics and inducing factors, MYC is a promising target for therapeutic molecules. Although it has been regarded as an undrugable target, its stability tightly regulated by the ubiquitin–proteasome system offers a new direction for molecule targeting and cancer treatment. Herein we report our discoveries in this research area, and we have found that deubiquitinase USP45 can directly bind with MYC, resulting in its deubiquitination and stabilization. Further, USP45 overexpressing can upregulate MYC, and this overexpressing can significantly enhance cancer development, cancer cell stemness and drug resistance. Interestingly, without enhancing cancer development, MYC silencing with shRNA can only suppress USP45-induced stemness and drug resistance. Moreover, we have identified that USP45 can be specifically bound and inhibited by a natural small molecule (α-mangostin), in turn significantly suppressing USP45-induced stemness and drug resistance. Since USP45 is significantly expressed in cervical tumors, we have discovered that the combination of α-mangostin and doxorubicin can significantly inhibit USP45-induced cervical tumorigenesis in an animal model. In general, on the basis of our USP45 discoveries on its MYC deubiquitination and α-mangostin inhibition, suppressing USP45 has opened a new window for suppressing cancer development, stemness and drug resistance

A Network-Based Systematic Study for the Mechanism of the Treatment of Zhengs Related to Cough Variant Asthma

Traditional Chinese medicine (TCM) has shown significant efficacy in the treatment of cough variant asthma (CVA), a special type of asthma. However, there is shortage of explanations for relevant mechanism of treatment. As Zhengs differentiation is a critical concept in TCM, it is necessary to explain the mechanism of treatment of Zhengs. Based on TCM clinical cases, this study illustrated the mechanism of the treatment of three remarkably relevant Zhengs for CVA: “FengXieFanFei,” “FeiQiShiXuan”, and “QiDaoLuanJi.” To achieve this goal, five steps were carried out: (1) determining feature Zhengs and corresponding key herbs of CVA by analyses of clinical cases; (2) finding out potential targets of the key herbs and clustering them based on their functional annotations; (3) constructing an ingredient-herb network and an ingredient network; (4) identifying modules of the ingredient network; (5) illustrating the mechanism of the treatment by further mining the latent biological implications within each module. The systematic study reveals that the treatment of “FengXieFanFei,” “FeiQiShiXuan,” and “QiDaoLuanJi” has effects on the regulation of multiple bioprocesses by herbs containing different ingredients with functions of steroid metabolism regulation, airway inflammation, and ion conduction and transportation. This network-based systematic study will be a good way to boost the scientific understanding of mechanism of the treatment of Zhengs