2 research outputs found
Kinetics and Antioxidant Capacity of Proanthocyanidins Encapsulated in Zein Electrospun Fibers by Cyclic Voltammetry
The proanthocyanidins
encapsulated in zein (zein-PA) fibers was
via electrospinning technique. The kinetics and antioxidant capacity
of PA from zein fibers was investigated by cyclic voltammetry. Circular
dichroism was used to investigate the secondary structure change of
zein and its influence on the shape of fibers. The addition of PA
caused a significant increase in viscosity and made fibers wider.
These hydrogen bonds between zein and PA molecules would favor the
α-helix change and decrease the β-folds of zein in electrospinning
solutions, leading to a round-shaped tendency of fibers and enhancing
the thermal properties slightly. Zein-PA fibers showed high encapsulation
efficiency close to 100%, and the encapsulated PA retained its antioxidant
capacity in fibers. Zein-PA fibers showed a good controlled release
toward PA, and the predominant release of PA from fibers was Fickian
diffusion, which could be well described by first-order model and
Hixson–Crowell model
Improvement of the activity and thermostability of microbial transglutaminase by multiple-site mutagenesis
<p>Microbial transglutaminase (MTG) is an enzyme widely used in the food industry. Mutiple-site mutagenesis of <i>Streptomyces mobaraensis</i> transglutaminase was performed in <i>Escherichia coli</i>. According to enzymatic assay and thermostability study, among three penta-site MTG mutants (DM01-03), DM01 exhibited the highest enzymatic activity of 55.7 ± 1.4 U/mg and longest half-life at 50 °C (418.2 min) and 60 °C (24.8 min).</p