2 research outputs found
Enhanced Osseointegration of Porous Titanium Modified with Zeolitic Imidazolate Framework‑8
Nanoscale zeolitic imidazolate framework-8
(ZIF-8)-modified titanium
(ZIF-8@AHT) can enhance osteogenesis in vitro. In this study, we systematically
and quantitatively examined the effects of ZIF-8@AHT on osteogenesis,
and investigated its ability to form bone in vivo. First, we coated
various quantities of nanoscale ZIF-8 crystals on alkali- and heat-treated
titanium (AHT) by controlling the concentration of the synthesis solution.
We then characterized the ZIF-8@AHT materials using scanning electron
microscopy (SEM), powder X-ray diffraction (XRD), Fourier transform
infrared spectroscopy (FT-IR), and determination of the sessile drop
contact angle. To illustrate the combined effects of micro/nanotopography
and ZIF-8@AHT composition on bone regeneration, we cultured MC3T3-E1
preosteoblast cells on various titanium substrates in vitro by setting
pure titanium (Ti) and AHT as control groups. The ZIF-8@AHTs enhanced
cell bioactivity compared with AHT and Ti, as evidenced by increased
extracellular matrix (ECM) mineralization, collagen secretion and
the upregulated expression of osteogenic genes (<i>Alp</i>, <i>Col1</i>, <i>Opg</i>, and <i>Runx2</i>) and osteogenesis-related proteins (ALP and OPG). ZIF-8@AHT-1/8
exhibited better osteogenic activity compared with the other ZIF-8@AHT
groups investigated. We subsequently inserted Ti, AHT, and ZIF-8@AHT-1/8
implants into the healed first molars (M1s) of mice, and found that
ZIF-8@AHT-1/8 also promoted osseointegration at the bone–implant
interface. These results suggest that ZIF-8@AHT-1/8 has great potential
for practical application in implant modification
Enhanced Osseointegration of Porous Titanium Modified with Zeolitic Imidazolate Framework‑8
Nanoscale zeolitic imidazolate framework-8
(ZIF-8)-modified titanium
(ZIF-8@AHT) can enhance osteogenesis in vitro. In this study, we systematically
and quantitatively examined the effects of ZIF-8@AHT on osteogenesis,
and investigated its ability to form bone in vivo. First, we coated
various quantities of nanoscale ZIF-8 crystals on alkali- and heat-treated
titanium (AHT) by controlling the concentration of the synthesis solution.
We then characterized the ZIF-8@AHT materials using scanning electron
microscopy (SEM), powder X-ray diffraction (XRD), Fourier transform
infrared spectroscopy (FT-IR), and determination of the sessile drop
contact angle. To illustrate the combined effects of micro/nanotopography
and ZIF-8@AHT composition on bone regeneration, we cultured MC3T3-E1
preosteoblast cells on various titanium substrates in vitro by setting
pure titanium (Ti) and AHT as control groups. The ZIF-8@AHTs enhanced
cell bioactivity compared with AHT and Ti, as evidenced by increased
extracellular matrix (ECM) mineralization, collagen secretion and
the upregulated expression of osteogenic genes (<i>Alp</i>, <i>Col1</i>, <i>Opg</i>, and <i>Runx2</i>) and osteogenesis-related proteins (ALP and OPG). ZIF-8@AHT-1/8
exhibited better osteogenic activity compared with the other ZIF-8@AHT
groups investigated. We subsequently inserted Ti, AHT, and ZIF-8@AHT-1/8
implants into the healed first molars (M1s) of mice, and found that
ZIF-8@AHT-1/8 also promoted osseointegration at the bone–implant
interface. These results suggest that ZIF-8@AHT-1/8 has great potential
for practical application in implant modification