Sub-10-nm Pd Nanosheets with Renal Clearance for Efficient Near-Infrared Photothermal Cancer Therapy

通讯作者地址: Zheng, NF (通讯作者)Efficient renal clearance is of fundamentally important property of nanoparticles for their in vivo biomedical applications. In this work, we report the successful synthesis of ultra-small Pd nanosheets (SPNS) with an average diameter of 4.4 nm and their application in photothermal cancer therapy using a near infrared laser. The ultra-small Pd nanosheets have strong optical absorption in the NIR region and high photothermal conversion efficiency (52.0%) at 808 nm. After being surface-functionalized with reduced glutathione (GSH), the SPNS-GSH was administered to mice to investigate the biodistribution, photothermal efficacy and tumor ablation in vivo. The in vivo photothermal therapy studies clearly demonstrate that surface modification with GSH allows the nanosheets to exhibit prolonged blood circulation and thus high accumulation in tumors. Upon 808 nm NIR irradiation, the tumors can be completely ablated. More importantly, with the size below the renal filtration limit (<10 nm), the GSHylated Pd nanosheets can be nicely cleared from body through the renal excretion route and into urine. Together with the high efficacy of NIR photothermal therapy, the unique renal clearance properties make the ultra-small Pd nanosheets promising for practical use in photothermal cancer therapy.MOST of China 2011CB932403 2014CB932004 NSFC of China 21131005 2092510

Multifunctional ultrasmall Pd nanosheets for enhanced near-infrared photothermal therapy and chemotherapy of cancer

通讯作者地址: Zheng, NF 电子邮件地址:[email protected] (NIR) photothermal therapy has developed very quickly in recent years. However, its clinical applications are hindered by many practical problems, such as low accumulation in tumors, high laser power density and high biotoxicity in vivo. Herein, a versatile system combining chemotherapy with photothermal therapy for cancer therapy using ultrasmall Pd nanosheets (SPNS) has been developed. The SPNS can serve as pH-responsive drug carriers to efficiently deliver DOX into cancer cells and tumors. On the other hand, the coordinative loading of DOX on SPNS enhances its accumulation in tumor tissue. So we can efficiently ablate tumor using low-intensity laser radiation. Importantly, with ultrasmall size (similar to 4.4 nm), SPNS surface-functionalized with reduced glutathione (GSH) can be cleared from the body through the renal system into the urine. This cancer therapeutic nanosystem, which exhibits a significant synergistic effect and low systemic toxicity, has great potential for clinical applications.Ministry of Science and Technology of China 2011CB932403 2014CB932004 National Natural Science Foundation of China 2113100

Silica coating improves the efficacy of Pd nanosheets for photothermal therapy of cancer cells using near infrared laser

通讯作者地址: Zheng, NF (通讯作者), Xiamen Univ, State Key Lab Phys Chem Solid Surfaces, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 地址: 1. Xiamen Univ, State Key Lab Phys Chem Solid Surfaces, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 2. Xiamen Univ, Dept Chem, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 电子邮件地址: [email protected] does matter! The ultrathin nature of 1.8 nm-thick Pd nanosheets prevents them entering cells effectively. A 13-times enhancement in the cells' uptake of the Pd nanosheets has now been achieved by silica coating together with surface functionalization, therefore significantly improving their NIR photothermal cell-killing efficacy.NSFC 21021061 20925103 20923004 20871100 Fok Ying Tung Education Foundation 121011 MSTC 2009CB930703 2011CB932403 NSF of Fujian Province 2009J06005 Key Scientific Project of Fujian Province 2009HZ0002-

Synthesis of thiolated chitosan and preparation nanoparticles with sodium alginate for ocular drug delivery

Purpose: The goal of the present study was to synthesize mucoadhesive polymer -thiolated chitosan (TCS) from chitosan (CS), then prepared CS/TCS-sodium alginate nanoparticles (CS/TCS-SA NPs), determined which was more potential for ocular drug delivery. Methods: A new method for preparing TCS was developed, and the characteristics were determined using Fourier transform infrared spectroscopy and the degree of thiol immobilized was measured by Ellman&apos;s reagent. Human corneal epithelium (HCE) cells were incubated with different concentrations of TCS for 48 h to determine the cell viabilities. CS/ TCS-SA NPs were prepared and optimized by a modified ionic gelation method. The particle sizes, zeta potentials, Scanning electron microscopy images, mucoadhesion, in vitro cell uptake and in vivo studies of the two types of NP were compared. Results: The new method enabled a high degree of thiol substitution of TCS, up to 1,411.01±4.02 μmol/g. In vitro cytocompatibility results suggest that TCS is nontoxic. Compared to CS-SA NPs, TCS-SA NPs were more stable, with higher mucoadhesive properties and could deliver greater amounts of drugs into HCE cells in vitro and cornea in vivo. Conclusions: TCS-SA NPs have better delivery capability, suggesting they have good potential for ocular drug delivery applications

Preparation and In Vitro

For preventing premature drug release in neutral environment and avoiding them being trapped into the endosomal/lysosomal system, we developed a novel iron silicate@liposome hybrid (ILH) formulation, which can be used as a carrier to transport doxorubicin (DOX) in a pH-sensitive manner and to escape from endosomal/lysosomal trapping through “proton-sponge” effect. The high intensity of photoacoustic signal from in vitro photoacoustic imaging (PAI) experiments suggests that it is a promising candidate for PAI agent, providing the potential for simultaneously bioimaging and cancer-targeting drug delivery. Cytotoxicity of our formulation toward tumor cells was remarkably higher than free DOX (48.4±7.7% and 26.2±8.4%, P<0.001). Confocal laser scanning microscopy experiments showed the enhanced transportation and enrichment process of DOX in QSG-7703 cells. Taking together, we developed an easy approach to construct a multifunctional anticancer drug delivery/imaging system with a potency as a PAI agent. The strategy of combining drug carrier and imaging agent is an emerging platform for further construction of nanoparticle and may play a significant role in cancer therapy and diagnosis

Synthesis of magnetic, fluorescent and mesoporous core-shell-structured nanoparticles for imaging, targeting and photodynamic therapy

A synthetic method to prepare novel multifunctional core-shell-structured mesoporous silica nanoparticles for simultaneous magnetic resonance (MR) and fluorescence imaging, cell targeting and photosensitization treatment has been developed. Superparamagnetic magnetite nanoparticles and fluorescent dyes are co-encapsulated inside nonporous silica nanoparticles as the core to provide dual-imaging capabilities (MR and optical). The photosensitizer molecules, tetra-substituted carboxyl aluminum phthalocyanine (AlC(4)Pc), are covalently linked to the mesoporous silica shell and exhibit excellent photo-oxidation efficiency. The surface modification of the core-shell silica nanoparticles with folic acid enhances the delivery of photosensitizers to the targeting cancer cells that overexpress the folate receptor, and thereby decreases their toxicity to the surrounding normal tissues. These unique advantages make the prepared multifunctional core-shell silica nanoparticles promising for cancer diagnosis and therapy.NSFC[21021061, 20925103, 20871100]; Fok Ying Tung Education Foundation[121011]; NSF of Fujian Province[2009J06005]; Fundamental Research Funds for the Central Universities[2010121015]; Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministr

Amphiphilic modification and asymmetric silica encapsulation of hydrophobic Au-Fe3O4 dumbbell nanoparticles

通讯作者地址: Zheng, NF (通讯作者),电子邮件地址: [email protected] facile method is developed for amphiphilic surface modification and asymmetric silica encapsulation of hydrophobic Au–Fe3O4 dumbbell nanoparticles. The obtained asymmetric Janus nanocomposites display tunable wettability, enhanced catalysis and better cell internalization as compared to those with complete silica encapsulation.MOST of China 2011CB932403 2014CB932004 NSFC of China 21131005 20925103 21333008 2102106

Hollow Mesoporous Zirconia Nanocapsules for Drug Delivery

通讯作者地址: Tang, SH (通讯作者), Xiamen Univ, Coll Chem & Chem Engn, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China 地址: 1. Xiamen Univ, Coll Chem & Chem Engn, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China 2. Xiamen Univ, Coll Chem & Chem Engn, Dept Chem, Xiamen 361005, Peoples R China 电子邮件地址: [email protected] mesoporous zirconia nanocapsules (hm-ZrO2) with a hollow core/porous shell structure are demonstrated as effective vehicles for anti-cancer drug delivery. While the highly porous feature of the shell allows the drug, doxorubicin(DOX), to easily pass through between the inner void space and surrounding environment of the particles, the void space in the core endows the nanocapsules with high drug loading capacity. The larger the inner hollow diameter, the higher their DOX loading capacity. A loading of 102% related to the weight of hm-ZrO2 is achieved by the nanocapsules with an inner diameter of 385 nm. Due to their pH-dependent charge nature, hm-ZrO2 loaded DOX exhibit pH-dependent drug releasing kinetics. A lower pH offers a faster DOX release rate from hm-ZrO2. Such a property makes the loaded DOX easily release from the nanocapsules when up-taken by living cells. Although the flow cytometry reveals more uptake of hm-ZrO2 particles by normal cells, hm-ZrO2 loaded DOX release more drugs in cancer cells than in normal cells, leading to more cytotoxicity toward tumor cells and less cytotoxicity to healthy cells than free DOX.NSFC 20925103,20871100,20721001 Fok Ying Tung Education Foundation 121011 RFDP 200803841010 NSF of Fujian Province 2009J06005 Key Scientific Project of Fujian Province 2009HZ0002-

Controlled Formation of Concave Tetrahedral/Trigonal Bipyramidal Palladium Nanocrystals

通讯作者地址: Zheng, NF (通讯作者), Xiamen Univ, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China 地址: 1. Xiamen Univ, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China 2. Xiamen Univ, Dept Chem, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 电子邮件地址: [email protected] concave Pd nanocrystals with uniform diameter were successfully prepared in the presence of formaldehyde. While the outer surfaces of the as-prepared concave Pd nanocrystals are {111}, the faces concave toward the polyhedral. center are high-surface-energy {110} faces. The degree of concavity and therefore the percentage of {110} of the nanocrystals are tunable by varying the amount of formaldehyde and the reaction temperature. Owing to the existence of active {110} facets, the electrocatalytic activity of the concave Pd nanocrystals displays dependency on their degree of concavity.NSFC20871100,20721001 MSTC 92009CB930703 RFDP 200803841010 NSF 2009J06005 Fujian Province 2009HZ0002-

Enhancing the Photothermal Stability of Plasmonic Metal Nanoplates by a Core-Shell Architecture

通讯作者地址: Zheng, NF (通讯作者),Xiamen Univ, Coll Chem & Chem Engn, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China 地址: 1. Xiamen Univ, Coll Chem & Chem Engn, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R China 2. Xiamen Univ, Dept Chem, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 电子邮件地址: [email protected] core shell bimetal nanoplates: A facile seeded-growth strategy is developed to prepare uniform plasmonic Pd@Ag core-shell bimetallic nanoplates. The as-prepared Pd@Ag nanoplates are not only uniform in both size and shape, but also display tunable SPR properties and significantly enhanced photothermal stability as compared with 2D pure-Ag nanostructures. They can thus be readily used as stable substrates for MR surface-enhanced Raman scattering and as NIR absorbers for photothermal cancer therapy.NSFC 21021061,20925103,20923004,20871100 Fok Ying Tung Education Foundation 121011 MOST of China 2011CB932403,2009CB930703 NSF of Fujian Province 2009J06005 Key Scientific Project of Fujian Province 2009HZ0002-