31 research outputs found
Intentions to Seek Information About E-Cigarettes: Perceived Risk, Efficacy, and Smoking Identity
10.1080/10810730.2021.1943728Journal of Health Communication265339-34
Calculation of Internal Force and Deformation of Overlying Soil Corrugated Arch Bridge Based on Plate and Shell Theory
Fluorescent Azobenzene-Containing Compounds: From Structure to Mechanism
The reversible photoisomerization of azobenzenes has been extensively studied to construct systems with optical responsiveness; however, this process limits the luminescence of these compounds. Recently, there have been many efforts to design and synthesize fluorescent azobenzene compounds, such as inhibition of electron transfer, inducing aggregation, and metal-enhancement, which make the materials ideal for application in fluorescence probes, light-emitting devices, molecular detection, etc. Herein, we review the recently reported progress in the development of various fluorescent azobenzenes and summarize the possible mechanism of their fluorescence emission. The potential applications of these materials are also discussed. Finally, in order to guide research in this field, the existing problems and future development prospects are discussed
Conditioned medium from hypoxic bone marrow-derived mesenchymal stem cells enhances wound healing in mice.
Growing evidence indicates that bone marrow-derived mesenchymal stem cells (BM-MSCs) enhance wound repair via paracrine. Because the extent of environmental oxygenation affects the innate characteristics of BM-MSCs, including their stemness and migration capacity, the current study set out to elucidate and compare the impact of normoxic and hypoxic cell-culture conditions on the expression and secretion of BM-MSC-derived paracrine molecules (e.g., cytokines, growth factors and chemokines) that hypothetically contribute to cutaneous wound healing in vivo. Semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) analyses of normoxic and hypoxic BM-MSCs and their conditioned medium fractions showed that the stem cells expressed and secreted significantly higher amounts of basic fibroblast growth factor (bFGF),vascular endothelial growth factor A (VEGF-A) interleukin 6 (IL-6) and interleukin 8 (IL-8) under hypoxic conditions. Moreover, hypoxic BM-MSC-derived conditioned medium (hypoCM) vs. normoxic BM-MSC-derived conditioned medium (norCM) or vehicle control medium significantly enhanced the proliferation of keratinocytes, fibroblasts and endothelial cells, the migration of keratinocytes, fibroblasts, endothelial cells and monocytes, and the formation of tubular structures by endothelial cells cultured on Matrigel matrix. Consistent with these in vitro results, skin wound contraction was significantly accelerated in Balb/c nude mice treated with topical hypoCM relative to norCM or the vehicle control. Notably increased in vivo cell proliferation, neovascularization as well as recruitment of inflammatory macrophages and evidently decreased collagen I, and collagen III were also found in the hypoCM-treated group. These findings suggest that BM-MSCs promote murine skin wound healing via hypoxia-enhanced paracrine
Angiopoietin-1 is associated with a decreased risk of lymph node metastasis in early stage cervical cancer
Objectives. Lymph node metastasis (LNM) is
an important determinant of prognosis in patients with
cervical cancer. Members of the angiopoietin family
have been demonstrated to regulate tumor-associated
angiogenesis and lymphangiogenesis. This study aimed
to investigate the expression levels of angiopoietin-1
(ANG1) and angiopoietin-2 (ANG2) in clinically early
stage of cervical cancer along with their correlations
with LNM.
Methods. In total, 124 human cervical cancer cases
classified into stage IA-IIB in accordance with the
International Federation of Gynecology and Obstetrics
(FIGO) 2009 staging criteria were included. ANG1 and
ANG2 expression levels in the tumor sections were
assessed by immunohistochemistry (IHC). Univariate
and multivariate logistic regression models, including
age at diagnosis, FIGO stage, tumor size, pathological
type, histological grading, depth of stromal invasion,
lymph-vascular space invasion (LVSI) and the
expression status of ANG1 and ANG2, were used to
evaluate the odds ratios (ORs) for LNM.
Results. ANG1 and ANG2 were positively
expressed in 75 (60.5%) and 89 (71.8%) cervical cancers
respectively, with predominant staining in the cytoplasm.
ANG1 expression was significantly decreased in tumors
with LNM, while no correlation was observed between
ANG2 expression and LNM. More importantly, the
multivariate logistic regression analysis demonstrated
that high ANG1 expression was an independent
protective factor of LNM (OR 0.107, 95% confidential
interval [CI] 0.020~0.567), while LVSI was an
independent risk factor of LNM (OR 34.313, 95% CI
5.914~199.092).
Conclusion. ANG1 is associated with a significantly
decreased risk of LNM in early stage cervical cancer.
The predictive value and role of ANG1 in LNM needs to
be further investigated in future studies
Correction: Conditioned Medium from Hypoxic Bone Marrow-Derived Mesenchymal Stem Cells Enhances Wound Healing in Mice
Giant Enhancements of Perpendicular Magnetic Anisotropy and Spin-Orbit Torque by a MoS2 Layer
Giant Enhancements of Perpendicular Magnetic Anisotropy and Spin-Orbit Torque by a MoS2 Laye
Increased capillary-like tube formation stimulated by BM-MSC hypoCM.
<p>(A) HUVECs were seeded onto a Matrigel matrix and incubated with vehicle control medium, hypoCM or norCM for 12 h. (B)Tube formation was quantified. Scale bar, 400 µm for images in (A) (200×). Results are given as the means ± the SEM; *<i>p</i>< 0.05 compared with the vehicle control or the norCM group.</p