11 research outputs found
Oral administration of interferon-Ī±2b-transformed Bifidobacterium longum protects BALB/c mice against coxsackievirus B3-induced myocarditis
Multiple reports have claimed that low-dose orally administered interferon (IFN)-Ī± is beneficial in the treatment of many infectious diseases and provides a viable alternative to high-dose intramuscular treatment. However, research is needed on how to express IFN stably in the gut. Bifidobacterium may be a suitable carrier for human gene expression and secretion in the intestinal tract for the treatment of gastrointestinal diseases. We reported previously that Bifidobacterium longum can be used as a novel oral delivery of IFN-Ī±. IFN-transformed B. longum can exert an immunostimulatory role in mice; however the answer to whether this recombinant B. longum can be used to treat virus infection still remains elusive. Here, we investigated the efficacy of IFN-transformed B. longum administered orally on coxsackie virus B3 (CVB3)-induced myocarditis in BALB/c mice. Our data indicated that oral administration of IFN-transformed B. longum for 2 weeks after virus infection reduced significantly the severity of virus-induced myocarditis, markedly down regulated virus titers in the heart, and induced a T helper 1 cell pattern in the spleen and heart compared with controls. Oral administration of the IFN-transformed B. longum, therefore, may play a potential role in the treatment of CVB3-induced myocarditis
Synthesis and biological evaluation of 12-N-p-chlorobenzyl sophoridinol derivatives as a novel family of anticancer agents
Taking 12-N-p-chlorobenzyl sophoridinol 2 as a lead, a series of novel sophoridinic derivatives with various 3ā²-substituents at the 11āside chain were synthesized and evaluated for their anticancer activity from sophoridine (1), a natural antitumor medicine. Among them, the sophoridinic ketones 5aāb, alkenes 7aāb and sophoridinic amines 14aāb displayed reasonable antiproliferative activity with IC50 values ranging from 3.8 to 5.4Ā Ī¼mol/L. Especially, compounds 5a and 7b exhibited an equipotency in both adriamycin (AMD)-susceptible and resistant MCF-7 breast carcinoma cells, indicating a different mechanism from AMD. The primary mechanism of action of 5a was to arrest the cell cycle at the G0/G1 phase, consistent with that of parent compound 1. Thus, we consider 12-chlorobenzyl sophoridinic derivatives with a tricyclic scaffold to be a new class of promising antitumor agents with an advantage of inhibiting drug-resistant cancer cells
The Genetic Polymorphisms in the MIR17HG Gene Are Associated with the Risk of Head and Neck Squamous Cell Carcinoma in the Chinese Han Population
Purpose. Head and neck squamous cell carcinoma (HNSCC) is the most common malignant tumors in the world. Genetic variants have an important role in HNSCC progression. Our study is aimed at exploring the relationship between MIR17HG polymorphisms and HNSCC risk in the Chinese Han population. Methods. We recruited 537 HNSCC cases and 533 healthy subjects to detect the correlation of six polymorphisms in MIR17HG with HNSCC susceptibility. The associations were evaluated by computing odds ratios (ORs) and 95% confidence intervals (CIs) using logistic regression analysis. Results. Our study revealed that rs7336610 (OR 1.77, 95%CI=1.09ā2.86, and p=0.021) and rs1428 (OR 1.73, 95%CI=1.07ā2.81, and p=0.025) are strongly associated with increased susceptibility to HNSCC in men. Besides, rs17735387 played a crucial protective role in stage III/IV HNSCC patients (OR 0.34, 95%CI=0.12ā0.95, and p=0.040) compared with stage I/II. Conclusion. Our study firstly indicated that MIR17HG polymorphisms are significantly associated with HNSCC susceptibility, which suggests that MIR17HG has a potential role in the occurrence of HNSCC
Magnetic Nanotag-Based Colorimetric/SERS Dual-Readout Immunochromatography for Ultrasensitive Detection of Clenbuterol Hydrochloride and Ractopamine in Food Samples
Direct and sensitive detection of multiple illegal additives in complex food samples is still a challenge in on-site detection. In this study, an ultrasensitive immunochromatographic assay (ICA) using magnetic Fe3O4@Au nanotags as a capture/detection difunctional tool was developed for the direct detection of Ī²2-adrenoceptor agonists in real samples. The Fe3O4@Au tag is composed of a large magnetic core (~160 nm), a rough Au nanoshell, dense surface-modified Raman molecules, and antibodies, which cannot only effectively enrich targets from complex solutions to reduce the matrix effects of food samples and improve detection sensitivity, but also provide strong colorimetric/surface-enhanced Raman scattering (SERS) dual signals for ICA testing. The dual readout signals of the proposed ICA can meet the detection requirements in different environments. Specifically, the colorimetric signal allows for rapid visual detection of the analyte, and the SERS signal is used for the sensitive and quantitative detection modes. The proposed dual-signal ICA can achieve the simultaneous determination of two illegal additives, namely, clenbuterol hydrochloride and ractopamine. The detection limits for the two targets via colorimetric and SERS signals were down to ng mLā1 and pg mLā1 levels, respectively. Moreover, the proposed assay has demonstrated high accuracy and stability in real food samples
Magnetically Assisted Surface-Enhanced Raman Spectroscopy for the Detection of Staphylococcus aureus
Magnetically Assisted Surface-Enhanced Raman Spectroscopy for the Detection of Staphylococcus aureus Based on Aptamer Recognition
A magnetically
assisted surface-enhanced Raman scattering (SERS)
biosensor for single-cell detection of S. aureus on the basis of aptamer recognition is reported for the first time.
The biosensor consists of two basic elements including a SERS substrate
(Ag-coated magnetic nanoparticles, AgMNPs) and a novel SERS tag (AuNRāDTNB@AgāDTNB
coreāshell plasmonic NPs or DTNB-labeled inside-and-outside
plasmonic NPs, DioPNPs). Uniform, monodisperse, and superparamagnetic
AgMNPs with favorable SERS activity and magnetic responsiveness are
synthesized by using polymer polyethylenimine. AgMNPs use magnetic
enrichment instead of repeated centrifugation to prevent sample sedimentation.
DioPNPs are designed and synthesized as a novel SERS tag. The Raman
signal of DioPNPs is 10 times stronger than that of the commonly used
SERS tag AuNRāDTNB because of the double-layer DTNB and the
LSPR position adjustment to match the given laser excitation wavelength.
Consequently, a strong SERS enhancement is achieved. Under the optimized
aptamer density and linker length, capture by aptamer-modified AgMNPs
can achieve favorable bacteria arrest (up to 75%). With the conventional
Raman spectroscopy, the limit of detection (LOD) is 10 cells/mL for S. aureus detection, and a good linear relationship
is also observed between the SERS intensity at Raman peak 1331 cm<sup>ā1</sup> and the logarithm of bacteria concentrations ranging
from 10<sup>1</sup> to 10<sup>5</sup> cells/mL. With the help of the
newly developed SERS mapping technique, single-cell detection of S. aureus is easily achieved
Synthesis, Biological Evaluation, and Autophagy Mechanism of 12<i>N</i>āSubstituted Sophoridinamines as Novel Anticancer Agents
A series
of 12<i>N</i>-substituted sophoridinamine derivatives
were synthesized and evaluated for their cytotoxic activities in human
HepG2 hepatoma cells. Structureāactivity relationship revealed
that introduction of a suitable arylidene or arylethyl at the <i>N</i>ā²-end could greatly enhance antiproliferation potency.
Among them, compound <b>6b</b> possessing a <i>N</i>ā²-trimethoxyphenyl methylene exhibited potent antiproliferation
effect against three human tumor cell lines including HepG2, leukemia
(K562), and breast cancer (HMLE), with IC<sub>50</sub> between 0.55
and 1.7 Ī¼M. The underlying mechanism of <b>6b</b> against
tumor cells is to block autophagic flux, mainly through neutralizing
lysosomal acidity. Our results indicated that compound <b>6b</b> is a potent lysosomal deacidification agent and is accordingly able
to block autophagic flux and inhibit tumor cell growth