HEPATOPROTECTIVE EFFECT OF PHYLLANTHUS NIRURI ALKALOID FRACTION IN D–GALACTOSAMINE INDUCED HEPATITIS IN RATS

Objective: The current study was designed to explore the hepatoprotective activity of Phyllanthus niruri (PN) alkaloid fraction in induced hepatitis in rats.Methods: The rats are divided into 5 groups as per the experimental design. Pre alkaloid treatment of PN was given to the hepatitis rats for 21 d. After completion of the treatment, the serum was collected, and biochemical analysis was carried out. The hepatic markers like alanine aminotransferase (ALT), aspartate aminotransferase (AST) activities, bilirubin (BL) urea (UR), creatinine (CR) levels are estimated in serum.Results: ALT, AST activities, BL, CR levels are increased, and UR levels are decreased in hepatitis rats. However with PN alkaloid fraction pre-treatment all these hepatic markers significantly (P<0.01) came back to near normal levels. Histopathological studies also prove that PN protected the hepatic tissue from hepatitis.Conclusion: The outcome of the present study showed that the alkaloid fraction of PN possesses hepatoprotective effect in hepatitis rats.Keywords: Phyllanthus niruri, Hepatitis, Liver marker

Exploring the Natural Preservation Potential of Aqueous Guava Leaf Extracts on Pangasius Pangasius: An Experimental Study

Fish, being a significant biological indicator in water, serves as a valuable food source once harvested. However, the high protein content in fish makes it an ideal medium for microorganisms, which can lead to spoilage. In areas without access to freezers or ice, preserving fish becomes a challenge. The objective of this study is to investigate the efficacy of Psidium guajava (guava) leaves as a natural preservation method for Pangasius pangasius fish. Key parameters, including gills, eye, texture, odor, and mucilage, were used to evaluate fish quality. Leaf methanolic extract was applied at doses of 0%, 20%, 40%, 60%, and 80%. Data were collected between 1 and 3 days after storage. Results showed that fish quality declined and began to deteriorate after 2 days of storage, particularly in the control treatment (0% extract). However, the fish samples treated with doses of 60% and 80% experienced relatively good quality over the course of 2 days. Although some spoilage occurred in these samples, they remained suitable for consumption. In contrast, fish samples treated with other doses exhibited complete spoilage and were no longer consumable. In conclusion, guava leaf extracts offer a promising alternative for fish preservation

Assessment of Potential Antioxidant Activity of Polyphenolic Fraction Separated from Acalypha Indica Leaves: An In vitro Approach

ABSTRACT The free radicals are generated in normal cellular metabolic events .However they may be produced more in disease conditions when they directly interact with the bio molecules are dangerous to the normal life because they alter genetic material. Free radicals induce permanent modifications in genetic material that may lead to mutagenesis, carcinogenesis and aging. The prevention or treatment of diseases has direct link with antioxidants which can reduce the effect of free radicals. The phenolic compounds are functionally potent antioxidants and they have the ability to scavenge reactive oxygen species (ROS)/ reactive nitrogen species(RNS), suppress the ROS/RNS formation by inhibiting relevant enzymes, chelates trace metal elements responsible for free radical generation and up regulates or protects the antioxidant defense system. In this study, we attempted for the separation of Polyphenolic fraction from Acalypha indica leaves and tested for its antioxidant activity employing DPPH, H2O2 assays and also to assess its reducing power, total antioxidant power. The outcome of the results from this study showed that Polyphenolic fraction from the Acalypha indica leaves scavenges the in vitro free radicals to their stable forms such as DPPH radical to DPPH, Mo (VI) to Mo (V), ferric cyanide complex to ferrous form and hydrogen peroxide to water. The polyphenolic fraction has the reduction power against free radicals which suggests further studies to take up in in vivo for the treatment of various diseases due to free radicals generation