Protein-Polyelectrolyte Complexes and Micellar Assemblies.

In this review, we highlight the recent progress in our understanding of the structure, properties and applications of protein-polyelectrolyte complexes in both bulk and micellar assemblies. Protein-polyelectrolyte complexes form the basis of the genetic code, enable facile protein purification, and have emerged as enterprising candidates for simulating protocellular environments and as efficient enzymatic bioreactors. Such complexes undergo self-assembly in bulk due to a combined influence of electrostatic interactions and entropy gains from counterion release. Diversifying the self-assembly by incorporation of block polyelectrolytes has further enabled fabrication of protein-polyelectrolyte complex micelles that are multifunctional carriers for therapeutic targeted delivery of proteins such as enzymes and antibodies. We discuss research efforts focused on the structure, properties and applications of protein-polyelectrolyte complexes in both bulk and micellar assemblies, along with the influences of amphoteric nature of proteins accompanying patchy distribution of charges leading to unique phenomena including multiple complexation windows and complexation on the wrong side of the isoelectric point

Understanding the Values of Live Game Streaming: a Value-Focused Thinking Approach

Today, along with the development of information communication technology, live streaming is getting more and more popular in China. It has established an integrated industrial chain. There are a large number of users watching different live streams (e.g., games. sports, live shows) through personal computers and mobile devices. In this paper, we aim to investigate the values of live game streaming using the Value-Focused Thinking (VFT) approach. We analyze the values of live game streaming from the perspectives of both streamers and spectators. The identified values are represented in the form of a means-ends objective network. The means-ends objective network derived from this research can serve as a conceptual foundation for future studies and provide useful guidelines for practitioners to better operate live game streaming applications

A high-throughput WSN for structural health monitoring

A challenge with existing WSNs used for structural health monitoring (SHM) is how to increase the data transmission rate (DTR) for large amounts of sampling data. To handle this issue, this paper proposes a new design method of a high-throughput WSN with multi-radio sink node (M-RSN) which can increase the data transfer ability of WSN. Additionally, a tight scheduled approach and multi-radio time synchronization method are designed for the stable implementation of the proposed network. A high data throughput of 1020 Kbps of the developed network has been proved. To evaluate the effectiveness and robustness of the proposed network designing method, experiments for aircraft composite wing boxes monitoring are carried out. The evaluation results have shown the advantages of the proposed methods