Incidence and Etiology of Drug-Induced Liver Injury in Mainland China

Background & Aims: We performed a nationwide, retrospective study to determine the incidence and causes of drug-induced liver injury (DILI) in mainland China.Methods: We collected data on a total of 25,927 confirmed DILI cases, hospitalized from 2012 through 2014 at 308 medical centers in mainland China. We collected demographic, medical history, treatment, laboratory, disease severity, and mortality data from all patients. Investigators at each site were asked to complete causality assessments for each case whose diagnosis at discharge was DILI (n=29,478) according to the Roussel Uclaf Causality Assessment Method.Results: Most cases of DILI presented with hepatocellular injury (51.39%; 95% CI, 50.76–52.03), followed by mixed injury (28.30%; 95% CI, 27.73–28.87) and cholestatic injury (20.31%; 95% CI, 19.80–20.82). The leading single classes of implicated drugs were traditional Chinese medicines or herbal and dietary supplements (26.81%) and anti-tuberculosis medications (21.99%). Chronic DILI occurred in 13.00% of the cases and, although 44.40% of the hepatocellular DILI cases fulfilled Hy’s Law criteria, only 280 cases (1.08%) progressed to hepatic failure, 2 cases underwent liver transplantation (0.01%), and 102 patients died (0.39%). Among deaths, DILI was judged to have a primary role in 72 (70.59%), a contributory role in 21 (20.59%), and no role in 9 (8.82%). Assuming the proportion of DILI in the entire hospitalized population of China was represented by that observed in the 66 centers where DILI capture was complete, we estimated the annual incidence in the general population to be 23.80 per 100,000 persons (95% CI, 20.86–26.74). Only hospitalized patients were included in this analysis, so the true incidence is likely to be higher.Conclusions: In a retrospective study to determine the incidence and causes of drug-induced liver injury (DILI) in mainland China, the annual incidence in the general population was estimated to be 23.80 per 100,000 persons—higher than that reported from western countries. Traditional Chinese medicines, herbal and dietary supplements, and anti-tuberculosis drugs were the leading causes of DILI in mainland Chin

Study on the Seismic Behavior of a Steel Plate–Concrete Composite Shear Wall with a Fishplate Connection

The steel plate–concrete composite shear wall (SPCSW), having been widely applied to several super high-rise buildings, is currently regarded as a new type of lateral load-resisting structure. The SPCSW design does not consider the connection to the surrounding structure, normally envisaged as a buttweld connection, while the fishplate lap connection tends to be applied in construction. To explore the fishplate lap connection to achieve the performance standard of SPCSW, in this paper, an SPCSW with a fishplate connection is modeled using ABAQUS to investigate the hysteretic behavior under constant axial force and horizontal cyclic loads. Through the hysteresis curve and a load–displacement skeleton curve, the effects of fishplate thickness and lap length on its hysteretic behavior are studied. The results show that increasing the fishplate thickness contributes to a slight increase in the bearing capacity and energy dissipation and has little influence on stiffness degradation. When the fishplate thickness is more than half the steel plate thickness, the strength and energy dissipation of an SPCSW with a fishplate connection can reach the level of an SPCSW without a fishplate connection. The bearing capacity and stiffness of the SPCSW increase with the increase in lap length. When the lap length is greater than 50 mm, the strength, stiffness and energy dissipation capacity of an SPCSW with a fishplate connection are superior to those without fishplate connections. Finally, engineering suggestions on fishplate connections are put forward

Study on the Seismic Behavior of a Steel Plate–Concrete Composite Shear Wall with a Fishplate Connection

The steel plate–concrete composite shear wall (SPCSW), having been widely applied to several super high-rise buildings, is currently regarded as a new type of lateral load-resisting structure. The SPCSW design does not consider the connection to the surrounding structure, normally envisaged as a buttweld connection, while the fishplate lap connection tends to be applied in construction. To explore the fishplate lap connection to achieve the performance standard of SPCSW, in this paper, an SPCSW with a fishplate connection is modeled using ABAQUS to investigate the hysteretic behavior under constant axial force and horizontal cyclic loads. Through the hysteresis curve and a load–displacement skeleton curve, the effects of fishplate thickness and lap length on its hysteretic behavior are studied. The results show that increasing the fishplate thickness contributes to a slight increase in the bearing capacity and energy dissipation and has little influence on stiffness degradation. When the fishplate thickness is more than half the steel plate thickness, the strength and energy dissipation of an SPCSW with a fishplate connection can reach the level of an SPCSW without a fishplate connection. The bearing capacity and stiffness of the SPCSW increase with the increase in lap length. When the lap length is greater than 50 mm, the strength, stiffness and energy dissipation capacity of an SPCSW with a fishplate connection are superior to those without fishplate connections. Finally, engineering suggestions on fishplate connections are put forward

Co-expression of nitrogenase proteins in cotton (Gossypium hirsutum L.)

Chemical nitrogen fertilizer can maintain crop productivity, but overuse of chemical nitrogen fertilizers leads to economic costs and environmental pollution. One approach to reduce use of nitrogen fertilizers is to transfer nitrogenase biosynthetic pathway to non-legume plants. Fe protein encoded by nifH and MoFe protein encoded by nifD and nifK are two structural components of nitrogenase. NifB encoded by nifB is a critical maturase that catalyzes the first committed step in the biosynthesis of nitrogenase FeMo-cofactor that binds and reduces N2. Expression of the nifB, nifH, nifD and nifK is essential to generate plants that are able to fix atmospheric N2. In this study, the four genes (nifB, nifH, nifD and nifK) from Paenibacillu polymyxaWLY78 were assembled in plant expression vector pCAMBIA1301 via Cre/LoxP recombination system, yielding the recombinant expression vector pCAMBIA1301-nifBHDK. Then, the four nif genes carried in the expression vector were co-introduced into upland cotton R15 using Agrobacterium tumefaciens-mediated transformation. Homozygous transgenic cotton lines B2, B5 and B17 of T3 generation were selected by PCR and RT-PCR. qRT-PCR showed that nifB, nifH, nifD and nifK were co-expressed in the transgenic cottons at similar levels. Western blotting analysis demonstrated that NifB, NifH, NifD and NifK were co-produced in the transgenic cottons. Co-expression of the four critical Nif proteins (NifB, NifH, NifD and NifK) in cottons represents an important step in engineering nitrogenase biosynthetic pathway to non-legume plants

Improvements of dielectric properties of Cu doped LaTiO3+δ

The ceramic composites of Cu-doped La1−xCuxTiO3+δ (x=0.05, 0.15, 0.3, 0.5) were synthesized by conventional solid-state reaction. The complex dielectric properties of the composites were investigated as a function of temperature (77 K≤T≤320 K) and frequency (100 Hz≤f≤1 MHz) separately. In all composites, the dielectric constants increase monotonously and the dielectric loss undulates with temperature. And it is clearly observed that extraordinarily high low-frequency dielectric constant (~104) appear at room temperature in La0.5Cu0.5TiO3+δ, which is ~100 times larger than that of La0.95Cu0.05TiO3+δ. Interestingly, the dielectric constants increase remarkably with the doped Cu contents, meanwhile the dielectric loss for all samples is ideal lower than 1 at room temperature in the measured frequency range. By means of complex impedance analysis, the improvements of dielectric properties are attributed to both bulk contribution and grain boundary effect, in which the bulk polaronic relaxation and the Maxwell–Wagner relaxation due to grain boundary response are heightened remarkably with the high doped Cu contents

Diversity of non- Saccharomyces yeasts of grape berry surfaces from representative Cabernet Sauvignon vineyards in Henan Province, China

International audienceNon-Saccharomyces yeasts are important players during winemaking and may come from grapes grown in vineyards. To study the diversity of non-Saccharomyces yeasts on grape berry surfaces, 433 strains were isolated from different Cabernet Sauvignon vineyards grown in Henan Province. Our results demonstrated that these strains were classified into 16 morphotypes according to their growth morphology on Wallerstein Laboratory agar medium, and were identified as seven species from four genera—Hanseniaspora opuntiae, Hanseniaspora vineae, Hanseniaspora uvarum, Pichia occidentalis, Pichia kluyveri, Issatchenkia terricola and Saturnispora diversa—based on a series of molecular biological experiments. Hanseniaspora opuntiae was obtained from all sampling sites except Changyuan County, while Pichia kluyveri and Saturnispora diversa were only found in sites of Zhengzhou Grape Resource Garden and Minquan County, respectively. The site Minquan was home of the greatest species richness, while only one single species (Hanseniaspora opuntiae) was detected at NAPA winery from Zhengzhou or at Anyang County. Finally, this study suggested that the geographic distribution and diversity of non-Saccharomyces yeast populations on Cabernet Sauvignon grape berries were likely to be determined by a combination of grape varieties and environmental factors

Preparation and characterization of acetylsalicylic acid/chitosan nanoparticles and its antithrombotic effects

Chitosan (CS)-acetylsalicylic acid (ASA) nanoparticles, which are well dispersed and stable in aqueous solution, have been prepared by interpolymer complexation of ASA in CS solution. The physicochemical properties of nanoparticles were investigated by using FT-IR, 1H NMR, scanning electron microscope（SEM）, dynamic light scattering, and UV spectrophotometer. It was found that the carboxyl group of the ASA had firmly integrated on the amino group of CS and the ASA-CS nanoparticles were almost spherical in shape with an average diameter of less than (79.3 ± 24.6) nm in high reproducibility and showed high chemical stability against environmental changes. It was also found that the prepared nanoparticles carried a positive charge and showed the size in the range from 700 to 150 nm. The surface structure and zeta potential of nanoparticles can be controlled by different preparation processes. The factor experiment results indicated that the ASA-CS nanoparticles had satisfactory loading capacity (LC) and encapsulation efficiency (EE), 27.27% and 46.88% (data not shown), respectively. The experiments of in vitro ASA release showed that these nanoparticles provided a sustained and pH-dependent drug release manner, and the release behavior was influenced by the pH value of the medium. Preliminary pharmacology experiment exhibited prolonged circulation and higher bioavailability than that of ASA. All the results indicated that ASA/CS nanoparticles may have promising pharmaceutical application, and further pharmacological research is needed to confirm these beneficial effects

AlScN-Based Dual-Mode Devices with Temperature Compensated Strategy and Process Optimization

MEMS resonators with interdigital transducer (IDT) based on aluminum nitride (AlN) thin-film (a few micrometers thickness) materials gradually arouse researchers\u27 interest due to their ability to excite multiple modes of acoustic waves. However, the low electromechanical coupling coefficient is still one of the main reasons that limit the AlN-based resonators. In this article, we demonstrate a design journey for 10% scandium-doped AlN-based dual-mode acoustic wave resonators. Also, we investigated the influence of stack architectures with and without introduced AlN interlays, as well as the influence of stacked film thicknesses, on the performance of resonators. Simulation results show that changes in the SiO2 film thickness have different effects on the TCF of the two acoustic wave modes, providing a way for sensors to decouple two parameters that jointly affect the frequency drift. Moreover, measured results show that the introduced AlN interlayer (AlN-IL) induces a better crystalline orientation of the scandium-doped AlN (AlScN) thin film and a higher coupling coefficient of the leaky longitudinal (LL) mode (K2 : from 4.19% to 4.96%), which make the resonators have great potential in surface acoustic wave (SAW)-based applications such as sensors and high-frequency applications

UniInst: Unique Representation for End-to-End Instance Segmentation

Existing instance segmentation methods have achieved impressive performance but still suffer from a common dilemma: redundant representations (e.g., multiple boxes, grids, and anchor points) are inferred for one instance, which leads to multiple duplicated predictions. Thus, mainstream methods usually rely on a hand-designed non-maximum suppression (NMS) post-processing step to select the optimal prediction result, which hinders end-to-end training. To address this issue, we propose a box-free and NMS-free end-to-end instance segmentation framework, termed UniInst, that yields only one unique representation for each instance. Specifically, we design an instance-aware one-to-one assignment scheme, namely Only Yield One Representation (OYOR), which dynamically assigns one unique representation to each instance according to the matching quality between predictions and ground truths. Then, a novel prediction re-ranking strategy is elegantly integrated into the framework to address the misalignment between the classification score and the mask quality, enabling the learned representation to be more discriminative. With these techniques, our UniInst, the first FCN-based end-to-end instance segmentation framework, achieves competitive performance, e.g., 39.0 mask AP using ResNet-50-FPN and 40.2 mask AP using ResNet-101-FPN, against mainstream methods on COCO test-dev. Moreover, the proposed instance-aware method is robust to occlusion scenes, outperforming common baselines by remarkable mask AP on the heavily-occluded OCHuman benchmark. Our codes will be available upon publication.Comment: This work is in the revision phase of the journal Neurocomputing. Codes will be available upon publicatio

View from the Biological Property: Insight into the Functional Diversity and Complexity of the Gut Mucus

Due to mucin’s important protective effect on epithelial tissue, it has garnered extensive attention. The role played by mucus in the digestive tract is undeniable. On the one hand, mucus forms “biofilm” structures that insulate harmful substances from direct contact with epithelial cells. On the other hand, a variety of immune molecules in mucus play a crucial role in the immune regulation of the digestive tract. Due to the enormous number of microorganisms in the gut, the biological properties of mucus and its protective actions are more complicated. Numerous pieces of research have hinted that the aberrant expression of intestinal mucus is closely related to impaired intestinal function. Therefore, this purposeful review aims to provide the highlights of the biological characteristics and functional categorization of mucus synthesis and secretion. In addition, we highlight a variety of the regulatory factors for mucus. Most importantly, we also summarize some of the changes and possible molecular mechanisms of mucus during certain disease processes. All these are beneficial to clinical practice, diagnosis, and treatment and can provide some potential theoretical bases. Admittedly, there are still some deficiencies or contradictory results in the current research on mucus, but none of this diminishes the importance of mucus in protective impacts