EGFR phosphorylation regulates HCV internalization but is not required for the recruitment of clathrin and AP-2μ1.

(A, C, E, and G) Huh-7.5 spheroids were incubated with DMSO or 5 μM AG-1478 for 2 hr, infected with DiD-HCV (red) with DMSO or AG-1478 for 1 hr at 4°C, shifted to 37°C for the indicated times, fixed, and probed for ZO-1 (A), CLDN1 (C), clathrin light chain (clathrin LC) (E) or AP-2μ1 (G) (green). (B, D, F, and H) Quantitation of (A), (C), (E) and (G), respectively. n = total DiD signal. Mean +/- SEM. **p < 0.01.</p