Ovarian granulosa cell tumor in a Persian cat: Case report from Tripoli, Libya

Background: Ovarian granulosa cell tumors (GCTs) are common neoplasms in domestic animals that originate from the sex-cord stromal cells of the ovary. The aim of the present paper was to report a case of ovarian GCT in a 5-year-old Persian female cat and to describe the histopathological pattern of this tumor.Case Description: The cat was brought for a routine diagnostic examination for pregnancy at Albaittar clinic in Tripoli, Libya with a history of 1 month mating before it was brought to the clinic for investigation of progressive behavioral changes. The cat external examination showed noticeable enlargement in the abdomen that was potentially suspected of pregnancy; however, the abdominal ultrasonography showed a great mass located on the right ovary. A therapeutic ovariohysterectomy was performed. The cat’s clinical signs resolved, 6 months later it was diagnosed with a mass on the right kidney suspected as metastasis and one week later the cat died.Conclusion: In this case, macroscopic and microscopic studies are discussed and histopathological examination confirmed ovarian GCT with cystic endometrial hyperplasia and suspected metastasis in one kidney

Effects of octylphenol on sexual development and reproduction in zebrafish

Anthropogenic chemicals released into the aquatic environment can disrupt the normal function(s) of an organism's endocrine system and thereby adversely affect reproduction and development. This has led to the development of robust fish tests to detect and assess endocrine active chemicals. This thesis investigated developmental and reproductive effects of exposure to octylphenol (OP) in zebrafish. Zebrafish were exposed to OP in a Fish Sexual Development Test (FSDT) to investigate effects on sexual development. The main endpoints were vitellogenin induction and gonad development, including sex ratios. Zebrafish were also exposed to OP in a Fish Full Life Cycle test (FFLC) and a Fish Short Term Reproduction Assay (FSTRA) to investigate effects on different reproductive processes; i.e. gonad development, sexual phenotype and reproductive performance. Exposures to OP resulted in shifts in sex ratios, suppression in ovarian development, impairment in reproduction and reduction in growth, whereas no effects on VTG levels were observed. Conclusively, both the FSDT and FFLC test, but not the FSTRA, were sensitive tests for detection of endocrine-related effects of the weak estrogen OP. This thesis shows that OP, acting as a weak estrogen, has a negative impact on sexual development and maturation as well as reproduction in zebrafish

Pathological lesions associated with Vibrio infection in Atlantic horse mackerel (Trachurus trachurus L., 1758) from the Western Coast of Tripoli, Libya

Background: Vibrios are aquatic bacteria and ubiquitous in the marine environment causing Vibriosis in humans and aquatic animals. Vibriosis is a significant disease of cultured and wild fish living in marine water, brackish, and freshwater, which causes highly economic losses. Aim: The present study was aimed to address the impact of Vibrios on the health status of Trachurus trachurus inhabiting coastal area of Tripoli. Methods: A total of 100 samples of (Trachurus trachurus) were collected randomly from the Western Coast of Tripoli and Bab Al- Baher market, throughout the period from spring 2019 to summer 2019.All the sampled fish were examined externally and internally to identify any potential lesions. Bacterial isolation from liver and kidney was carried out using the appropriate culture media. Tissue samples were taken from liver, kidney and spleen in 10% neutral buffered formalin for histopathology examination. The tissue sections were stained with Hematoxylin and Eosin (H&E) to assess morphologically and Perl's Prussian blue for the demonstration of ferric iron. Results: On average 69% of the infected fish exhibited at least one pathological lesion. Vibrios were recovered from 90% of the examined fishes. The histopathological changes in the liver were observed as severe congestion of blood vessels, mononuclear cell infiltration surrounding a bile duct, granular degeneration and coagulative necrosis of hepatocytes in the centrilobular area, marked vacuolar degeneration of hepatocytes, activation of melano-macrophages centers (MMCs), multiple cysts of nematode in hepatic tissue (incidental finding). With respect to kidney and spleen, examination of H & E stained tissue sections have severe congestion of blood vessels, vacuolar degeneration of the renal tubular epithelium, severe interstitial mononuclear cell infiltration, and marked activation of melano-macrophages centers (MMCs) in the kidney. Moreover, congestion of blood vessels and marked activation of melano-macrophages (MMCs) were found in the spleen. The melano-macrophage centers (MMCs) of most of the sampled tissues showed a strong positive reaction for ferric iron. Conclusion: The polluted aquatic environment by sewage at Tripoli Coast is an essential factor for triggering the pathogenicity and invasion of Vibrios to vulnerable Atlantic horse mackerel fishes. Also, this study are a preliminary step to give a baseline for further future studies on epidemiology and control of Vibrio infection in Libyan fish. [Open Vet J 2023; 13(3.000): 327-336