Fabrication and Evaluation of Pluripotent Stem Cells Expressing in Human iPS Cells as In-vitro Model

Our latest report details the production of human induced pluripotent stem cells (iPSC) master cell banks (MCB) that are produced by a therapeutically compliant approach that starts with cord blood. The two iPSC clones created by this method were thoroughly characterised in this publication, utilising whole genome sequencing (WGS), microarray, and comparative genomic hybridization (aCGH) single nucleotide polymorphism (SNP) analysis. We compare these profiles with lines created by a similar procedure from different donors, a reporter subclone, and a suggested calibration material. We think it is likely that several clinical products will be made using iPSCs. Furthermore, given their immortal state, we anticipate that the lines utilised as input material will be used for many years or even decades at various sites. As a result, assay development will be crucial to tracking the condition of the cells and their drift within the culture. We propose that a thorough description of the cells' starting state, a comparison with some calibration data, and the creation of reporter sublcones will aid in identifying the most beneficial set of tests for keeping an eye on the cells and defining standards for eliminating a line

Biological activities of leaves of ethnomedicinal plant, Borassus flabellifer Linn. (Palmyra palm): An antibacterial, antifungal and antioxidant evaluation

Plants contain a variety of phytochemicals that have the ability to exert effect on human body. Among them Borassus flabellifer Linn. is a medicinally important plant. In traditional medicine different parts of plants are being used for their medicinal properties. The methanol extract was obtained from powdered leaves and further fractions were prepared. Antimicrobial potential was investigated using eight pathogenic strains of bacteria and fungi by agar well diffusion method. Broth dilution method was employed to MIC and MMC of active samples and MIC index value was determined. ME was subjected to preliminary phytochemical analysis; and 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydrogen peroxide (H2O2) radical scavenging activity. Phytochemical screening revealed the presence of several phytochemicals. The ME showed dose dependent radical scavenging activity as evidenced by IC50 values for DPPH (40.19 μg/ml) and H2O2 (30.92 μg/ml) radicals. The inhibition zones and MIC values for bacterial strains were in the range of 10–16 mm and 50–70 μg/ml, respectively. All the samples showed an inhibitory effect on fungal strains with inhibition zone (10–17 mm) and MFC (50–70 μg/ml). Samples exhibited diverse patterns of antibacterial and antifungal effects. Among the tested samples, methanol extract and acetone fraction (AF) had potent antibacterial and antifungal activities. These results lead to the conclusion that the plant has a broad spectrum antimicrobial and antioxidant activity and could be a potential option for treating various infectious diseases. The strong antioxidant property of methanol extract might be employed in the development of natural antioxidants for agro-food and pharmaceutical industries

Quorum sensing: an under-explored phenomenon in the phylum Actinobacteria

Quorum sensing is known to play a major role in the regulation of secondary metabolite production, especially antibiotics, and morphogenesis in the phylum Actinobacteria. Although it is one of the largest bacterial phylum, only 25 of the 342 genera have been reported to use quorum sensing. Of these, only nine have accompanying experimental evidence; the rest are only known through bioinformatic analysis of gene/genome sequences. It is evident that this important communication mechanism is not extensively explored in Actinobacteria. In this review, we summarize the different quorum sensing systems while identifying the limitations of the existing screening strategies and addressing the improvements that have taken place in this field in recent years. The γ-butyrolactone system turned out to be almost exclusively limited to this phylum. In addition, methylenomycin furans, AI-2 and other putative AHL-like signaling molecules are also reported in Actinobacteria. The lack of existing screening systems in detecting minute quantities and of a wider range of signaling molecules was a major reason behind the limited information available on quorum sensing in this phylum. However, recent improvements in screening strategies hold a promising future and are likely to increase the discovery of new signaling molecules. Further, the quorum quenching ability in many Actinobacteria has a great potential in controlling the spread of plant and animal pathogens. A systematic and coordinated effort is required to screen and exploit the enormous potential that quorum sensing in the phylum Actinobacteria has to offer for human benefit

Development and modification of the tragacanth solid lipid nanoparticles with natural polymer

Nanobiocomposite recycling is straightforward because nanofillers' physical properties do not modify throughout processing because of their considerable thermal stability. Additionally, the low nanofiller loading has no notable effect on the density of nanobiocomposite during an elevated appearance ratio, designate that nanobiocomposite has a very elevated prospective for use. Under mild situation, we present an easy technique for fabricating silver nanoparticles spontaneously in the presence of gum tragacanth polymer (a natural polymer) without the use of a conventional reducing agent. Silver nanoparticles were formulated by mixing equal quantities of tragacanth and silver nitrate aqueous solutions at 0.5 weight percent. UV–visible, Fourier transform infrared, X-ray diffraction and X-ray photoelectron spectroscopy were utilized to substantiate the production of silver nanoparticles. Gum tragacanth polymeric chains aid in the reduction process and serve as superior stabilisers throughout the course of six months. A transmission electron microscope was utilized to substantiate the nanoparticle production and stability. The use of organic solvents or reducing agents is not required in the formation of silver nanoparticles among this approach