5 research outputs found
Cell-Imprinted Substrates Direct the Fate of Stem Cells
Smart nanoenvironments were obtained by cell-imprinted substrates based on mature and dedifferentiated chondrocytes as templates. Rabbit adipose derived mesenchymal stem cells (ADSCs) seeded on these cell-imprinted substrates were driven to adopt the specific shape (as determined in terms of cell morphology) and molecular characteristics (as determined in terms of gene expression) of the cell types which had been used as template for the cell-imprinting. This method might pave the way for a reliable, efficient, and cheap way of controlling stem cell differentiation. Data also suggest that besides residual cellular fragments, which are presented on the template surface, the imprinted topography of the templates plays a role in the differentiation of the stem cells
Cell-Imprinted Substrates Modulate Differentiation, Redifferentiation, and Transdifferentiation
Differentiation
of stem cells into mature cells through the use
of physical approaches is of great interest. Here, we prepared smart
nanoenvironments by cell-imprinted substrates based on chondrocytes,
tenocytes, and semifibroblasts as templates and demonstrated their
potential for differentiation, redifferentiation, and transdifferentiation.
Analysis of shape and upregulation/downregulation of specific genes
of stem cells, which were seeded on these cell-imprinted substrates,
confirmed that imprinted substrates have the capability to induce
specific shapes and molecular characteristics of the cell types that
were used as templates for cell-imprinting. Interestingly, immunofluorescent
staining of a specific protein in chondrocytes (i.e., collagen type
II) confirmed that adipose-derived stem cells, semifibroblasts, and
tenocytes can acquire the chondrocyte phenotype after a 14 day culture
on chondrocyte-imprinted substrates. In summary, we propose that common
polystyrene tissue culture plates can be replaced by this imprinting
technique as an effective and promising way to regulate any cell phenotype
in vitro with significant potential applications in regenerative medicine
and cell-based therapies
Cell-Imprinted Substrates Act as an Artificial Niche for Skin Regeneration
Bioinspired materials
can mimic the stem cell environment and modulate
stem cell differentiation and proliferation. In this study, biomimetic
micro/nanoenvironments were fabricated by cell-imprinted substrates
based on mature human keratinocyte morphological templates. The data
obtained from atomic force microscopy and field emission scanning electron microscopy revealed that the keratinocyte-cell-imprinted poly(dimethylsiloxane) casting procedure could imitate the surface morphology of the plasma membrane, ranging from the nanoscale to the macroscale, which may provide the required topographical cell fingerprints to induce differentiation. Gene expression levels of the genes analyzed (involucrin, collagen type I, and keratin 10) together with protein expression data showed that human adipose-derived stem cells (ADSCs) seeded on these cell-imprinted substrates were driven to adopt the specific shape and characteristics of keratinocytes. The observed morphology of the ADSCs grown on the keratinocyte casts was noticeably different from that of stem cells cultivated on the stem-cell-imprinted substrates. Since the shape and geometry of the nucleus could potentially alter the gene expression, we used molecular dynamics to probe the effect of the confining geometry on the chain arrangement of simulated chromatin fibers in the nuclei. The results obtained suggested that induction of mature cell shapes onto stem cells can influence nucleus deformation of the stem cells followed by regulation of target genes. This might pave the way for a reliable, efficient, and cheap approach of controlling stem cell differentiation toward skin cells for wound healing applications
Cell-Imprinted Substrates Act as an Artificial Niche for Skin Regeneration
Bioinspired materials
can mimic the stem cell environment and modulate
stem cell differentiation and proliferation. In this study, biomimetic
micro/nanoenvironments were fabricated by cell-imprinted substrates
based on mature human keratinocyte morphological templates. The data
obtained from atomic force microscopy and field emission scanning electron microscopy revealed that the keratinocyte-cell-imprinted poly(dimethylsiloxane) casting procedure could imitate the surface morphology of the plasma membrane, ranging from the nanoscale to the macroscale, which may provide the required topographical cell fingerprints to induce differentiation. Gene expression levels of the genes analyzed (involucrin, collagen type I, and keratin 10) together with protein expression data showed that human adipose-derived stem cells (ADSCs) seeded on these cell-imprinted substrates were driven to adopt the specific shape and characteristics of keratinocytes. The observed morphology of the ADSCs grown on the keratinocyte casts was noticeably different from that of stem cells cultivated on the stem-cell-imprinted substrates. Since the shape and geometry of the nucleus could potentially alter the gene expression, we used molecular dynamics to probe the effect of the confining geometry on the chain arrangement of simulated chromatin fibers in the nuclei. The results obtained suggested that induction of mature cell shapes onto stem cells can influence nucleus deformation of the stem cells followed by regulation of target genes. This might pave the way for a reliable, efficient, and cheap approach of controlling stem cell differentiation toward skin cells for wound healing applications
Cell-Imprinted Substrates Act as an Artificial Niche for Skin Regeneration
Bioinspired materials
can mimic the stem cell environment and modulate
stem cell differentiation and proliferation. In this study, biomimetic
micro/nanoenvironments were fabricated by cell-imprinted substrates
based on mature human keratinocyte morphological templates. The data
obtained from atomic force microscopy and field emission scanning electron microscopy revealed that the keratinocyte-cell-imprinted poly(dimethylsiloxane) casting procedure could imitate the surface morphology of the plasma membrane, ranging from the nanoscale to the macroscale, which may provide the required topographical cell fingerprints to induce differentiation. Gene expression levels of the genes analyzed (involucrin, collagen type I, and keratin 10) together with protein expression data showed that human adipose-derived stem cells (ADSCs) seeded on these cell-imprinted substrates were driven to adopt the specific shape and characteristics of keratinocytes. The observed morphology of the ADSCs grown on the keratinocyte casts was noticeably different from that of stem cells cultivated on the stem-cell-imprinted substrates. Since the shape and geometry of the nucleus could potentially alter the gene expression, we used molecular dynamics to probe the effect of the confining geometry on the chain arrangement of simulated chromatin fibers in the nuclei. The results obtained suggested that induction of mature cell shapes onto stem cells can influence nucleus deformation of the stem cells followed by regulation of target genes. This might pave the way for a reliable, efficient, and cheap approach of controlling stem cell differentiation toward skin cells for wound healing applications