Axillary lymph node of the same patient showing metastatic ductal carcinoma breast with central necrosis closely mimicking DCIS with comedo necrosis, H & E, Mag: 2×

<p><b>Copyright information:</b></p><p>Taken from "Infiltrating ductal carcinoma breast with central necrosis closely mimicking ductal carcinoma in situ (comedo type): a case series"</p><p>http://www.jmedicalcasereports.com/content/1/1/83</p><p>Journal of Medical Case Reports 2007;1():83-83.</p><p>Published online 8 Sep 2007</p><p>PMCID:PMC2014768.</p><p></p

Same tumor as shown in Fig 1 stained with a cocktail of myoepithelial markers

<p><b>Copyright information:</b></p><p>Taken from "Infiltrating ductal carcinoma breast with central necrosis closely mimicking ductal carcinoma in situ (comedo type): a case series"</p><p>http://www.jmedicalcasereports.com/content/1/1/83</p><p>Journal of Medical Case Reports 2007;1():83-83.</p><p>Published online 8 Sep 2007</p><p>PMCID:PMC2014768.</p><p></p> Note absent myoepithelial layer consistent with the diagnosis of infiltrating ductal carcinoma with central necrosis. Mag: 10×

Altered expression of cell cycle and apoptotic proteins in chronic hepatitis C virus infection-1

and 4 (F4) (Mayer hematoxylin, magnification 400×).<p><b>Copyright information:</b></p><p>Taken from "Altered expression of cell cycle and apoptotic proteins in chronic hepatitis C virus infection"</p><p>http://www.biomedcentral.com/1471-2180/8/133</p><p>BMC Microbiology 2008;8():133-133.</p><p>Published online 5 Aug 2008</p><p>PMCID:PMC2518161.</p><p></p

Altered expression of cell cycle and apoptotic proteins in chronic hepatitis C virus infection-4

<p><b>Copyright information:</b></p><p>Taken from "Altered expression of cell cycle and apoptotic proteins in chronic hepatitis C virus infection"</p><p>http://www.biomedcentral.com/1471-2180/8/133</p><p>BMC Microbiology 2008;8():133-133.</p><p>Published online 5 Aug 2008</p><p>PMCID:PMC2518161.</p><p></p

Altered expression of cell cycle and apoptotic proteins in chronic hepatitis C virus infection-3

Was observed principally in sinusoidal lining cells (arrow) and rarely in hepatocytes (arrow head), b) Bcl-2 staining observed in inflammatory cells, (magnification 400×).<p><b>Copyright information:</b></p><p>Taken from "Altered expression of cell cycle and apoptotic proteins in chronic hepatitis C virus infection"</p><p>http://www.biomedcentral.com/1471-2180/8/133</p><p>BMC Microbiology 2008;8():133-133.</p><p>Published online 5 Aug 2008</p><p>PMCID:PMC2518161.</p><p></p

DataSheet_1_Immune mapping of human tuberculosis and sarcoidosis lung granulomas.docx

Tuberculosis (TB) and sarcoidosis are both granulomatous diseases. Here, we compared the immunological microenvironments of granulomas from TB and sarcoidosis patients using in situ sequencing (ISS) transcriptomic analysis and multiplexed immunolabeling of tissue sections. TB lesions consisted of large necrotic and cellular granulomas, whereas “multifocal” granulomas with macrophages or epitheloid cell core and a T-cell rim were observed in sarcoidosis samples. The necrotic core in TB lesions was surrounded by macrophages and encircled by a dense T-cell layer. Within the T-cell layer, compact B-cell aggregates were observed in most TB samples. These B-cell clusters were vascularized and could contain defined B-/T-cell and macrophage-rich areas. The ISS of 40–60 immune transcripts revealed the enriched expression of transcripts involved in homing or migration to lymph nodes, which formed networks at single-cell distances in lymphoid areas of the TB lesions. Instead, myeloid-annotated regions were enriched in CD68, CD14, ITGAM, ITGAX, and CD4 mRNA. CXCL8 and IL1B mRNA were observed in granulocytic areas in which M. tuberculosis was also detected. In line with ISS data indicating tertiary lymphoid structures, immune labeling of TB sections expressed markers of high endothelial venules, follicular dendritic cells, follicular helper T cells, and lymph-node homing receptors on T cells. Neither ISS nor immunolabeling showed evidence of tertiary lymphoid aggregates in sarcoidosis samples. Together, our finding suggests that despite their heterogeneity, the formation of tertiary immune structures is a common feature in granulomas from TB patients.</p