4 research outputs found
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When and why do third parties punish outside of the lab? A cross-cultural recall study
Punishment can reform uncooperative behavior and hence could have contributed to humans’ ability to live in large-scale societies. Punishment by unaffected third parties has received extensive scientific scrutiny because third parties punish transgressors in laboratory experiments on behalf of strangers that they will never interact with again. Often overlooked in this research are interactions involving people who are not strangers, which constitute many interactions beyond the laboratory. Across three samples in two countries (United States and Japan; N = 1,294), we found that third parties’ anger at transgressors, and their intervention and punishment on behalf of victims, varied in real-life conflicts as a function of how much third parties valued the welfare of the disputants. Punishment was rare (1–2%) when third parties did not value the welfare of the victim, suggesting that previous economic game results have overestimated third parties’ willingness to punish transgressors on behalf of strangers.</p
Robust estimation of bacterial cell count from optical density
Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data
Recommended from our members
When and Why Do Third Parties Punish Outside of the Lab? A Cross-Cultural Recall Study
Punishment can reform uncooperative behavior and hence could have contributed to humans’ ability to live in large-scale societies. Punishment by unaffected third parties has received extensive scientific scrutiny because third parties punish transgressors in laboratory experiments on behalf of strangers that they will never interact with again. Often overlooked in this research are interactions involving people who are not strangers, which constitute many interactions beyond the laboratory. Across three samples in two countries (United States and Japan; N = 1,294), we found that third parties’ anger at transgressors, and their intervention and punishment on behalf of victims, varied in real-life conflicts as a function of how much third parties valued the welfare of the disputants. Punishment was rare (1–2%) when third parties did not value the welfare of the victim, suggesting that previous economic game results have overestimated third parties’ willingness to punish transgressors on behalf of strangers