Phytochemical Screening, Gas Chromatography-mass Spectrometry Analysis, and Antidiabetic Effects of Corchorus olitorius Leaves in Rats

BACKGROUND: Therapies for diabetes mellitus are still meeting failure in most cases, especially in the developed stages of the disease due to incredible associating complications. Hence, there is a need for continuous development of curative therapies for that stubborn disease. AIM: We aimed to investigate the antidiabetic effects of one of the most popular plants cultivated in Egypt, C. olitorius. METHODS: Phytochemical screening of total alcoholic extract of Corchorus olitorius leaves and its aqueous and chloroform fractions revealed the presence of flavonoids, saponins, carbohydrates, tannins, coumarins, and alkaloids. RESULTS: The gas chromatography-mass spectrometry analysis showed the presence of 12 and nine chemical compounds in aqueous and chloroform extracts, respectively. C. olitorius decreased serum glucose level and α-amylase activity. This effect was more pronounced in the total alcoholic extract and its chloroform fraction than the aqueous one. The extracts also adjusted the lipid profile, reduced liver injury parameters, and caused remarkable improvement and increase number, size, and density of functioning β-cells. CONCLUSION: The findings suggest the antihyperglycemic and antioxidant effects of C. olitorius besides its beneficial effect on diabetic complications such as hyperlipidemia and liver injury. The presence of some phytochemicals such as theophylline, trans-2, 3-dimethoxycinnamic acid, 7-hydroxy-4-methyl coumarin, apigenin 7-glucoside, and glycitein may contribute to such pharmacological effects

Effect of Dopant on the Nanostructured Morphology of Poly (1-naphthylamine) Synthesized by Template Free Method

The study reports some preliminary investigations on the template free synthesis of ascantlyinvestigated polyaniline (PANI) derivative—poly (1-naphthylamine) (PNA) by template free method in presence as well as absence of hydrochloric acid (HCl) (dopant), using ferric chloride as oxidant. The polymerization was carried out in alcoholic medium. Polymerization of 1-naphthylamine (NPA) was confirmed by the FT-IR as well as UV–visible studies. The morphology and size of PNA particles was strongly influenced by the presence and absence of acid which was confirmed by transmission electron microscopy (TEM) studies

Perception of motorcyclists on safe riding in Kota Kinabalu

Despite the high risks reflected from the annual road traffic casualties, motorcycles remain a popular mode of transport in Malaysia due to economic and ease-of-use factors. Therefore, various parties need to play their roles, make proper plans and take actions to accommodate the needs of the motorcyclists in Malaysia. Consequently, some fundamental basis on how motorcyclists interact with the road environment and other drivers was needed. For this reason, this study was to examine and assess motorcyclist perception towards safe riding regarding the road environments and other drivers' attitude and behaviour. A survey by distributing questionnaires among motorcyclists in Kota Kinabalu, Sabah was conducted and was analysed using Statistical Package for Social Science (SPSS) tools. It found that most motorcyclists, regardless of their demographic and riding background, perceive that poor road conditions such as road surface defects and slippery roads highly affect their safe motorcycling. Besides that, there was no statistically significant difference between motorcyclists' perceptions of positive and negative attitudes and behaviour. These findings also guide the development of any road safety initiatives and strategies, as well as for future studies specifically related to motorcycle concerns

Referrals to a facial pain service

AIM: To assess the quality of referral letters to a facial pain service and highlight the key requirements of such letters. METHOD: The source of all referral letters to the service for five years was established. For one year the information provided in 94 referrals was assessed. Using a predetermined checklist of essential information the referral letters were compared to these set criteria. RESULTS: The service received 7,001 referrals and, on average, general dental practitioners (GDPs) referred 303 more patients per year than general medical practitioners (GMPs). Seventy-one percent of all referrals were from primary care practitioners, the rest were from specialists. Over 70% of GMP and 52% of GDP letters included a past medical history, with GMPs more likely to suggest a possible diagnosis and include previous secondary care referrals. The mean score for GMP referrals compared to the standard proforma (maximum of 12) was 5.6 and for GDP referrals 5.0. A relevant drug history was included by 75.6% GMP compared to 38.7% of GDPs. GMPs were more likely to include any relevant mental health history. CONCLUSIONS: The overall quality of referral letters is low which makes it difficult for the specialists to provide robust treatment plans

Volumetric properties and resilient modulus of stone mastic asphalt incorporating cellulose fiber

Stone mastic asphalt (SMA) is well known as a high coarse aggregate content that interlocks to form a stone skeleton that resist permanent deformation. However, it facing a lot of problems such as rutting and stripping because of the high temperature and repeated axial load. It also suffers with creep issue. The cellulose fiber is a type of synthetic fiber that can enhance the properties of asphalt mixture. Thus, the aim of this study is to utilize the cellulose fiber that has high tensile strength to overcome the problem of SMA. Among the tests involve are Resilient Modulus, Marshall Stability and Cantabro Loss. From the results, it shows that the addition of cellulose fiber improved the recovery ability of asphalt binder. From each test, the addition of 0.2% cellulose fiber contributes to lowest value of abrasion, while 0.3% producing highest value of resilient modulus. Thus, the addition of cellulose fiber is capable in enhancing the properties of SMA

3D model simulation on magnetic induction spectroscopy for fetal acidosis detection using COMSOL multiphysics

Conventional fetal scalp blood sampling required an invasive measurement to detect fetal hypoxia in fetus. This paper describes non-invasive technique employing single channel magnetic induction technique as a solution to replace conventional technique nowadays. The objective of the simulation is to evaluate the magnetic induction spectroscopy setup for acidosis detection and to determine the most suitable types of coil to be used for better result in detecting acidosis in fetal blood. Result shows that primary field can be penetrates to the desired material and there are difference in pattern for both linear coil and circular coil. The result of the simulation is important and need to be considering in order developing the magnetic induction spectroscopy hardware to increase the accuracy of the system

The Use of Vacuum Residue as a Potential Rejuvenator in Reclaimed Asphalt Pavement: Physical, Rheological, and Mechanical Traits Analysis

Asphalt recycling technology with a high content of reclaimed asphalt pavement (RAP) is becoming more important as the price of paving materials rises and sustainable development and environmental conservation rules become more rigorous. Nevertheless, road authorities in numerous countries still prohibit the utilisation of RAP in asphalt mixes due to the negative impacts of RAP on the performance of asphalt mixtures. Consequently, different rejuvenators have been introduced to reinstate the original attributes of aged asphalt to resolve this issue. This study incorporated vacuum residue (VR) into mixtures with 40% RAP. The physical, rheological, and mechanical traits of the resultant samples were assessed. The results show that the 7.5% VR rejuvenator minimised the RAP asphalt ageing impact. Furthermore, the rejuvenating agent demonstrated physical and rheological rehabilitative benefits for the aged asphalt. The mechanical attributes of the rejuvenated samples were also enhanced compared to the virgin asphalt (VA) specimens

Extraction and Characterisation of Maltene from Virgin Asphalt as a Potential Rejuvenating Agent

The wide application of reclaimed asphalt pavement (RAP) is hindered due to the highly brittle nature of the material, which contributes a major factor towards cracking-related distresses. While the utilisation of rejuvenating agents has been shown to enhance the flexibility of RAP, they also trigger certain negative effects on the performance of asphalt mixtures. In view of this, potential rejuvenators should be able to alter the rheological properties of asphalts to limit fatigue issues and enhance the potential of low-temperature cracking. Therefore, this study aimed to investigate the possibility of extraction and characterisation of maltene from virgin asphalt (VA) as a potential rejuvenating agent in RAP. Several physicochemical characteristics were examined, including density, viscosity, gas chromatography–mass spectrometry (GC–MS), Fourier-transform infrared (FTIR) spectroscopy, CHNS elemental analysis, and energy dispersive X-ray (EDX) analysis. Finally, the stiffness modulus characteristics of the different types of asphalt binders were evaluated at low and high temperatures. The results demonstrated that maltene was successfully extracted from VA using petroleum ether. In addition, the GC–MS showed that the extracted maltene contained polar and non-polar compounds with low molecular weights compared to VA. Furthermore, the spectra curve of maltene was very similar to that of asphalt, indicating its compatibility with asphalt binder and prospective use. Finally, adding maltene to aged asphalt decreased stiffness values to 0.0063, 0.0499, and 0.0108 MPa, which are equivalent to VA values (0.0061, 0.0481, and 0.0104 MPa) at loading times of 1.0, 0.1, and 0.55 s, respectively. Meanwhile, the stiffness modulus characteristics at low temperature were restored with the addition of maltene

Gas1 Regulates Patterning of the Murine and Human Dentitions through Sonic Hedgehog

The mammalian dentition is a serially homogeneous structure that exhibits wide numerical and morphological variation among multiple different species. Patterning of the dentition is achieved through complex reiterative molecular signaling interactions that occur throughout the process of odontogenesis. The secreted signaling molecule Sonic hedgehog (Shh) plays a key role in this process, and the Shh coreceptor growth arrest-specific 1 (Gas1) is expressed in odontogenic mesenchyme and epithelium during multiple stages of tooth development. We show that mice engineered with Gas1 loss-of-function mutation have variation in number, morphology, and size of teeth within their molar dentition. Specifically, supernumerary teeth with variable morphology are present mesial to the first molar with high penetrance, while molar teeth are characterized by the presence of both additional and absent cusps, combined with reduced dimensions and exacerbated by the presence of a supernumerary tooth. We demonstrate that the supernumerary tooth in Gas1 mutant mice arises through proliferation and survival of vestigial tooth germs and that Gas1 function in cranial neural crest cells is essential for the regulation of tooth number, acting to restrict Wnt and downstream FGF signaling in odontogenic epithelium through facilitation of Shh signal transduction. Moreover, regulation of tooth number is independent of the additional Hedgehog coreceptors Cdon and Boc, which are also expressed in multiple regions of the developing tooth germ. Interestingly, further reduction of Hedgehog pathway activity in Shh$^{tm6Amc}$ hypomorphic mice leads to fusion of the molar field and reduced prevalence of supernumerary teeth in a Gas1 mutant background. Finally, we demonstrate defective coronal morphology and reduced coronal dimensions in the molar dentition of human subjects identified with pathogenic mutations in GAS1 and SHH/GAS1, suggesting that regulation of Hedgehog signaling through GAS1 is also essential for normal patterning of the human dentition