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    Cytomorphological investigations in Oxyria digyna Hill. from the Kashmir Himalaya, India

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    In the present paper, detailed cytomorphological investigations in Oxyria digyna Hill. from Kashmir Himalayaβ€”India have been reported for the first time. All the 14 investigated populations of O. digyna are diploid based on x = 7. Out of these in two populations 0–2B chromosomes have been recorded for the first time while 6 populations differed significantly in their meiotic characteristics. Meiotic abnormalities during male meiosis observed include inter PMC chromatin transfer (cytomixis). Non-synchronous disjunction of some bivalents, laggards and bridges at anaphases and telophases. Consequent to these meiotic anomalies, microsporogenesis in meiocytes is abnormal resulting in to dyads, triads and polyads with or without micronuclei. The overall effect is seen in reduced pollen fertility. Unreduced pollen grains were observed in some populations, which differed significantly in their size compared to the normal (reduced) pollen grains. It is observed that a smaller frequency of pollen grains differed morphologically in Aharbal and Yosmarg populations. The remaining eight populations showed regular meiotic course, normal microsporogenesis and high percentage of pollen fertility (95.09–99.09 %).ΠŸΡ€ΠΈΠ²ΠΎΠ΄ΡΡ‚ΡΡ Π΄Π΅Ρ‚Π°Π»ΡŒΠ½Ρ‹Π΅ цитоморфологичСскиС ис-слСдования Oxyria digyna Hill ΠΈΠ· ΠšΠ°ΡˆΠΌΠΈΡ€Π° (Π“ΠΈΠΌΠ°Π»Π°ΠΈ, Индия). ВсС 14 ΠΈΠ·ΡƒΡ‡Π΅Π½Π½Ρ‹Ρ… популяций ΡΠ²Π»ΡΡŽΡ‚ΡΡ Π΄ΠΈΠΏΠ»ΠΎΠΈΠ΄Π½Ρ‹ΠΌΠΈ, Π³Π΄Π΅ x = 7. Из Π½ΠΈΡ… Π² Π΄Π²ΡƒΡ… популяциях Π²ΠΏΠ΅Ρ€Π²Ρ‹Π΅ описаны 0–2B хромосомы, Ρ‚ΠΎΠ³Π΄Π° ΠΊΠ°ΠΊ ΡˆΠ΅ΡΡ‚ΡŒ популяций сильно Ρ€Π°Π·Π»ΠΈΡ‡Π°Π»ΠΈΡΡŒ ΠΏΠΎ своим мСйотичСским характСристикам. Аномалии ΠΌΠ΅ΠΉΠΎΠ·Π° ΠΏΡ€ΠΈ микроспорогСнСзС Π²ΠΊΠ»ΡŽΡ‡Π°Π»ΠΈ цитомиксис, нСсинхронноС расхоТдСниС Π½Π΅ΠΊΠΎΡ‚ΠΎΡ€Ρ‹Ρ… Π±ΠΈΠ²Π°Π»Π΅Π½Ρ‚ΠΎΠ², Π·Π°Π΄Π΅Ρ€ΠΆΠΊΠΈ ΠΈ мосты Π² Π°Π½Π°Ρ„Π°Π·Π°Ρ… ΠΈ Ρ‚Π΅Π»ΠΎΡ„Π°Π·Π°Ρ…. Π’ΠΎΠ·Π½ΠΈΠΊΠ°ΡŽΡ‰ΠΈΠΉ Π² связи с этим Π°Π½ΠΎΠΌΠ°Π»ΡŒΠ½Ρ‹ΠΉ микроспорогСнСз ΠΏΡ€ΠΈΠ²ΠΎΠ΄ΠΈΡ‚ ΠΊ Ρ„ΠΎΡ€ΠΌΠΈΡ€ΠΎΠ²Π°Π½ΠΈΡŽ Π΄ΠΈΠ°Π΄, Ρ‚Ρ€ΠΈΠ°Π΄ ΠΈ ΠΏΠΎΠ»ΠΈΠ°Π΄ ΠΊΠ°ΠΊ с микроядрами, Ρ‚Π°ΠΊ ΠΈ Π±Π΅Π· Π½ΠΈΡ…. ΠžΠ±Ρ‰ΠΈΠΌ эффСктом являСтся сниТСниС Ρ„Π΅Ρ€Ρ‚ΠΈΠ»ΡŒΠ½ΠΎΡΡ‚ΠΈ ΠΏΡ‹Π»ΡŒΡ†Ρ‹. Π’ Π½Π΅ΠΊΠΎΡ‚ΠΎΡ€Ρ‹Ρ… популяциях наблюдали Π½Π΅Ρ€Π΅Π΄ΡƒΡ†ΠΈΡ€ΠΎΠ²Π°Π½Π½Ρ‹Π΅ ΠΏΡ‹Π»ΡŒΡ†Π΅Π²Ρ‹Π΅ Π·Π΅Ρ€Π½Π°, ΠΊΠΎΡ‚ΠΎΡ€Ρ‹Π΅ ΠΏΠΎ Π²Π΅Π»ΠΈΡ‡ΠΈΠ½Π΅ Π·Π½Π°Ρ‡ΠΈΡ‚Π΅Π»ΡŒΠ½ΠΎ ΠΎΡ‚Π»ΠΈΡ‡Π°Π»ΠΈΡΡŒ ΠΎΡ‚ Π½ΠΎΡ€ΠΌΠ°Π»ΡŒΠ½Ρ‹Ρ…. Π’ популяциях Aharbal ΠΈ Yosmarg Π½Π΅ΠΊΠΎΡ‚ΠΎΡ€Ρ‹Π΅ ΠΏΡ‹Π»ΡŒΡ†Π΅Π²Ρ‹Π΅ Π·Π΅Ρ€Π½Π° ΠΎΡ‚Π»ΠΈΡ‡Π°Π»ΠΈΡΡŒ ΠΌΠΎΡ€Ρ„ΠΎΠ»ΠΎΠ³ΠΈ-чСски. ΠžΡΡ‚Π°Π²ΡˆΠΈΠ΅ΡΡ восСмь популяций проявляли Π½ΠΎΡ€ΠΌΠ°Π»ΡŒΠ½Ρ‹ΠΉ Ρ…ΠΎΠ΄ ΠΌΠ΅ΠΉΠΎΠ·Π°, Π½ΠΎΡ€ΠΌΠ°Π»ΡŒΠ½Ρ‹ΠΉ микроспорогСнСз ΠΈ высокий ΠΏΡ€ΠΎΡ†Π΅Π½Ρ‚ Ρ„Π΅Ρ€Ρ‚ΠΈΠ»ΡŒΠ½ΠΎΡΡ‚ΠΈ ΠΏΡ‹Π»ΡŒΡ†Ρ‹ (95,09–99,09 %).The authors are grateful to the University Grants Commission, New Delhi for providing financial assistance under the DRS SAP III and DST programmes. Thanks are also due to the Head, Department of Botany, Punjabi University, Patiala for necessary laboratory facilities
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