Wound healing activity of flaxseed (Linum usitatissimum) extract (water and ethanol) on human oral fibroblast cells (in vitro)

Flaxseed (Linum usitatissimum) plant has many health beneficial effects such as anti-microbial, anti-fungal and anti-cancer effects. In the current study, the wound healing activity of flaxseed (Linum usitatissimum) extract is being tested on oral fibroblast cell line. Flaxseed (Linum usitatissimum) is extracted using Soxhlet extraction method via two mediums water and ethanol to assess and compare the difference between the activities of both extracts; if present. After the extraction procedure, the extracts were tested for their biological activity using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay to determine the safety and dosage of the extract to be administered onto the oral fibroblast cells. Then, the wound healing assay is carried out to test the activity of both water and ethanol flaxseed extract on oral fibroblast cells. The results revealed that both water and ethanol extract have high wound healing activity against oral fibroblast cell line where ethanolic extract shows slightly higher activity in healing on oral fibroblast wounds (in vitro). One-way ANNOVA was carried out with post-Hoc Turkey test where the P value of compared variables was found to be < 0.05 at 95% confidence interval. Based on the results obtained; it could be concluded, that flaxseed extract has a potential wound healing activity as the results shows high wound healing activity on oral fibroblast cell line

The potential effect of different types of flaxseed (Linum usitatissimum) extract on the cell viability of oral fibroblasts human cell line

Herbal medication and natural products have been successfully demonstrated to have general health beneficial effects. The bioactivities of flaxseed (linum usitatissimum) extract have been reported, as previous studies have shown that l. usitatissimum extract has many health and beneficial effects such as antimicrobial, anti-oxidant and anti-inflammatory effect. L. usitatissimum extract makes a great skin wound healing agent in addition to that it has good effect on the oral cavity in treating ulcers and general oral health benefits. L. usitatissimum is extracted using ethanol in three different concentration via soxhlet method, gas chromatography mass spectrum (GC-MS) is used to illustrate the components of l. usitatissimum extract. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay is used to assess the oral fibroblasts cell viability in three different time lines. The results illustrate the major component present in l. usitatissimum extract and the effect of the different ethanol concentrations of flaxseed extract on oral fibroblasts human cell line. L. usitatissimum extract show proliferating effect, the 70% flaxseed ethanolic extract produced the highest proliferating effect on fibroblast cells at 24 hours followed by 100% followed by 90% ethanol flaxseed extract, at 48 hours and 72 hours 100% ethanoic extract produced the highest proliferating effect followed by 70% then 90% ethanol flaxseed extract. The results show proliferating effect by l. usitatissimum extract on human oral fibroblast cell line