PROBLEMS OF DESIGNING OF ADAPTABLE CLOTHES FOR PEOPLE WITH THE LIMITED IMPELLENT POSSIBILITIES

The requirement for special clothes and the footwear possessing special ergonomic functions, adapted for changing environmental conditions, activity of the person or its physical condition, gets the essential importance for rehabilitation of invalids as will allow to raise level of social security and quality of their life. The present work is spent in cooperation with the centre of rehabilitation of invalids of Tashkent where there takes place the treatment treatment people from various regions of Uzbekistan

Hepatitis E Virus SeroprevalencE among Different Age Groups of the Healthy Population of Kyrgyzstan

Knowledge regarding distribution of serological markers of Hepatitis E Virus (HEV) infection in the population of endemic regions is an important indicator of the public health and hygiene. The aim of this study was to determine seroprevalence of HEV among different age groups of the population in Kyrgyzstan regions. 648 serum samples of persons without liver pathology received in December 2014 to February 2015 was tested by immunoassay kit «DS-EIA-ANTI-HEV-G» (Diagnostic systems, Russia). IgG antibodies to HEV were detected in 4.8% (n = 31) of the examined samples. An analysis of the level of seropositivity in different regions and in different age groups of the population was made. The greatest number of seropositive persons were identified in Bishkek and Chuskaya oblast. The antibodies were detected most frequently in individuals aged 1 to 5, 15 to 19 years old and older than 50 years old. The obtained data indicate the constant virus circulation in Kyrgyzstan in interepidemic period

DESIGN OF HEPATITIS E VIRUS GENOTYPE 1 RECOMBINANT CAPSID PROTEIN: CLONING, EXPRESSION, PURIFICATION, EVALUATION OF THE ANTIGENIC PROPERTIES

Aim. The development of the hepatitis E virus (HEV) genotype 1 recombinant capsid protein. Materials and methods. Escherichia coli strains, plasmid vectors, serological and clinical samples, ELISA reagent kits, molecular biological, bioinformatic, biotechnological, biochemical and serological methods. Results. Using HEV genotype 1 DNA copy of subgenomic virus RNA we made E.coli strains producing recombinabt capsid protein, containing C-terminal fragment of ORF2 protein fused to E.coli beta-galactosidase. Recombinant protein ORF2 had been isolated from the inclusion bodies of the E.coli biomass and purified by size exclusion chromatography. By Western blotting it had been shown specific interaction of the recombinant polypeptide with anti-HEV IgG from pool of positive sera. Antigenic specificity of the recombinant polypeptide had been confirmed by enzyme-linked immunosorbent assay with sera of hepatitis E patients and reference groups: healthy donors, patients with hepatitis А, В, C, infectious mononucleosis and cytomegalovirus infection, HIV-infected patients. Conclusion. HEV genotype 1 ORF2 recombinant antigen had been developed, and its possible use in diagnostic tests had been experimentally shown

DESIGN OF HEPATITIS E VIRUS GENOTYPE 1 RECOMBINANT ORF3 PROTEIN BY CODON OPTIMIZATION METHOD

Aim. The development of the hepatitis E virus (HEV) genotype 1 full-size ORF3 recombinant polypeptide. Materials and methods. Escherichia coli strains, plasmid vectors, serological and clinical samples, ELISA reagent kits, molecular biological, bioinformatic, biotechnological, biochemical and serological methods. Results. HEV genotype 1 RNA had been isolated from clinical samples collected in Kyrgyzstan. DNA copy of subgenomic virus RNA had been cloned and used for further development of E.coli strains producing full-size recombinant protein ORF3 fused to E.coli beta-galactosidase. Codons optimization method was used in aim to increase expression level of recombinant protein. Recombinant protein ORF3 had been isolated from the inclusion bodies of the E.coli biomass and purified by size exclusion chromatography. Antigenic specificity of recombinant polypeptide had been confirmed by enzyme-linked immunosorbent assay and Western blotting with the specific sera. Conclusion. HEVgenotype 1 ORF3 recombinant antigen had been designed, and it’s applicability in diagnostic tests had been experimentally confirmed