Antiproliferative and cytotoxic activities of Mentha x piperita L. essential oil in non-small cell lung cancer cells

Among 33 types of listed cancers worldwide, lung cancer with 2.2 million cases (12.2% of total cancer cases) ranks second next only to breast cancer. Globally, Turkey, with overall rate of 40.0 (41,264 cases), ranks 5th among top 10 countries in lung cancer. Currently used therapeutic agents and approaches have considerable side effects, and hence, there is a need for alternative agents for effective management of lung cancer. In this study, we explored the in vitro cytotoxic, antiproliferative and proapoptotic activities of Mentha x piperita L. (peppermint) essential oil in human non-small cell lung cancer (A549) cells. Cell viability was determined by MTT assay, morphological changes were determined by confocal microscopy and apoptosis promoting action was determined by flow cytometry technique. Peppermint essential oil found to effectively decrease the viability of non-small cell lung cancer cells and IC50 value was detected at low concentrations (2.12%) for 24 h. In addition, peppermint essential oil was found to alter the morphology of A549 cells, leading to changes that could describe programmed cell death. Apoptosis was the triggered cell death by Mentha x piperita essential oil. Results reveal that Mentha x piperita essential oil has antiproliferative and anticarcinogenic properties which could be attributed to the bioactive phytochemical contents and has the potential to be used as an anticancer agent and chemotherapeutic drug.

Antiproliferative and cytotoxic activities of Mentha x piperita L. essential oil in non-small cell lung cancer cells

753-758Among 33 types of listed cancers worldwide, lung cancer with 2.2 million cases (12.2% of total cancer cases) ranks second next only to breast cancer. Globally, Turkey, with overall rate of 40.0 (41,264 cases), ranks 5th among the top 10 countries in lung cancer. Currently used therapeutic agents and approaches have considerable side effects, and hence, there is a need for alternative agents for effective management of lung cancer. In this study, we explored the in vitro cytotoxic, antiproliferative and proapoptotic activities of Mentha x piperita L. (peppermint) essential oil in human non-small cell lung cancer (A549) cells. Cell viability was determined by MTT assay, morphological changes were determined by confocal microscopy and apoptosis promoting action was determined by flow cytometry technique. Peppermint essential oil found to effectively decrease the viability of non-small cell lung cancer cells and IC50 value was detected at low concentrations (2.12%) for 24 h. In addition, peppermint essential oil was found to alter the morphology of A549 cells, leading to changes that could describe programmed cell death. Apoptosis was the triggered cell death by Mentha x piperita essential oil. Results reveal that Mentha x piperita essential oil has antiproliferative and anticarcinogenic properties which could be attributed to the bioactive phytochemical contents and has the potential to be used as an anticancer agent and chemotherapeutic drug

Investigation of in vitro biological activities of hollow mesoporous carbon nanopArticles bearing D-NMAPPD on human lung adenocarcinoma cells

The uniformly dispersed hollow mesoporous carbon nanopArticles (HMCNPs) were successfully synthesized by hard-template methods, and D-NMAPPD (B13) was successfully loaded onto the nanopArticle surface for the first time. Structural properties of bare and B13 loaded HMCNPs (HMCNs-B-13) were investigated by Fourier Transform Infrared Spectroscopy (FT-IR), Field Emission-Scanning Electron Microscopy (FE-SEM), Thermal Gravimetric Analysis (TG). The amount of drug released was determined via in vitro drug release studies at 37 degrees C in SBF through UV-Vis spectrometric and thermal analyses. TG data revealed that the proportion of loaded B-13 was 33.60%. Their ability to induce apoptosis in cultures of A549 human lung adenocarcinoma cells was investigated, and the inhibitory effect of HMCNPs-B-13 on lung cancer cell proliferation was determined in vitro. The IC50 values determined after application periods of 24 and 48 h were found to be 16.13 mu g/mL and 12.96 mu g/mL, respectively. The role of HMCNPs-B-13 on the morphology and ultrastructure of A549 cells was also investigated by confocal microscopy and Transmission electron microscopy (TEM) studies