An integrated expression atlas of miRNAs and their promoters in human and mouse

MicroRNAs (miRNAs) are short non-coding RNAs with key roles in cellular regulation. As part of the fifth edition of the Functional Annotation of Mammalian Genome (FANTOM5) project, we created an integrated expression atlas of miRNAs and their promoters by deep-sequencing 492 short RNA (sRNA) libraries, with matching Cap Analysis Gene Expression (CAGE) data, from 396 human and 47 mouse RNA samples. Promoters were identified for 1,357 human and 804 mouse miRNAs and showed strong sequence conservation between species. We also found that primary and mature miRNA expression levels were correlated, allowing us to use the primary miRNA measurements as a proxy for mature miRNA levels in a total of 1,829 human and 1,029 mouse CAGE libraries. We thus provide a broad atlas of miRNA expression and promoters in primary mammalian cells, establishing a foundation for detailed analysis of miRNA expression patterns and transcriptional control regions

Fat in a time of slim: the reinscription of race in the framing of fat desirability in post-apartheid South Africa

This article critically examines the way in which the reported sexual desire of black African men for fat women is contained and managed in South African media representations of fat. While sexual desire for fat women represents a potential challenge to the dominant framing of fat as diseased/dysfunctional/disgusting, the article shows how the reduction of this desire to one of two (racialized) ‘explanations’ – either evidence of racial primitivism or a (black male) strategy to avoid infection with HIV – emasculates the potentially powerful oppositional framing of fat as sexy. It is a mark of the dominant frame’s influence that it is capable of co-opting oppositional frames and recasting them in its own image. From the point of view of critiques of the fat-as-disease orthodoxy, the claim to the existence of an alternative norm of fat as sexually desirable in ‘black culture’ emerges as a problematic oppositional frame – saturated with raced assumptions in the way in which it is reported. The counter framing of the (black) fat body as sexually desirable is given column space to be derided and dismissed as an instance of deviant black sexuality, as a mistaken belief in need of ‘correction’, or it is subsumed under a medicalized frame as a strategy for the avoidance of disease rather than an expression of genuine sexual desire