In vitro fertilisation when normal sperm morphology is less than fifteen per cent: Results of in vitro fertilisation and embryo transfer at H. F. Verwoerd Hospital, Pretoria

The outcome of in vitro fertilisation and embryo transfer in 90 couples where the husband's normal sperm morphology was less than 15% were analysed. Based on the percentage of morphologically normal spermatozoa the patients were divided into three groups: group A - normal morphological features 0 - 5%; group B - 6 - 10%; and group C - 11 - 14%. A control group had normal morphological features ≥ 15%. The fertilisation rate and number of embryos transferred was not significantly different in these groups. However, the pregnancy rate per embryo transfer was significantly different for groups A and B compared with group C and the control group (0% and 5,9% for groups A and B v. 13,9% and 18,3% for group C and the control group, respectively). It is concluded that when the normal sperm morphology is less than 11% the prospect of a pregnancy is poor

In vitro culture of mouse embryos in human amniotic fluid

Human amniotic fluid was compared with Ham's F-10 culture medium as a possible alternative for use in in vitro fertilisation. The cleavage success of mouse embryos in human amniotic fluid (experimental group) was 92% compared with 86% in Ham's F-10 medium. It is concluded that human amniotic fluid is a viable alternative culture medium for mouse embryos