64 research outputs found

    Ascorbic acid increases demethylation in somatic cell nuclear transfer embryos of the pig ()

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    Objective Investigated the effect and mechanism of ascorbic acid on the development of porcine embryos produced by somatic cell nuclear transfer (SCNT). Methods Porcine embryos were produced by SCNT and cultured in the presence or absence of ascorbic acid. Ten-eleven translocation 3 (TET3) in oocytes was knocked down by siRNA injection. After ascorbic acid treatment, reprogramming genes were analyzed by realtime reverse transcription-polymerase chain reaction (RT-PCR). Furthermore, relative 5-methylcytosine and 5-hydroxymethylcytosine content in pronucleus were detected by realtime PCR. Results Ascorbic acid significantly increased the development of porcine embryos produced by SCNT. After SCNT, transcript levels of reprogramming genes, Pou5f1, Sox2, and Klf were significantly increased in blastocysts. Furthermore, ascorbic acid reduced 5-methylcytosine content in pronuclear embryos compared with the control group. Knock down of TET3 in porcine oocytes significantly prevents the demethylation of somatic cell nucleus after SCNT, even if in the presence of ascorbic acid. Conclusion Ascorbic acid enhanced the development of porcine SCNT embryos via the increased TET3 mediated demethylation of somatic nucleus

    Current Status of Old Housing for Low-Income Elderly Households in Seoul and Green Remodeling Support Plan: Economic Analysis Considering the Social Cost of Green Remodeling

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    In this study, the economic feasibility of green remodeling (GR), which could improve the health, safety, and energy of elderly households considering social cost, was analyzed. As a result, the net present value of GR was ‘−10,267 USD (49.7%)’, which was found to be uneconomical compared to the total construction cost (20,981 USD, 100%) despite benefits of energy saving, carbon reduction, and air pollutant reduction. Based on this result, in order to expand GR for low-income elderly households, who cannot afford to perform GR, a GR support measure linked to the currently implemented energy conversion and old-age housing support policies was proposed. It allows the government to perform GR for low-income elderly households with 1/4 of the total construction cost. This result could revitalize GR to reduce greenhouse gas and contribute to housing stability for low-income elderly households who are vulnerable to COVID-19 and climate change

    Current Status of Aged Public Buildings and Effect Analysis Prediction of Green Remodeling in South Korea

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    The purpose of this study is to analyze the scope of the expected improvement effect of office buildings and educational research facilities according to green remodeling. Thus, in order to quantitatively grasp the architectural performance of the existing buildings, the building thermal performance, the airtightness, the indoor environment, and the air quality were measured using equipment. The analysis indicated that the envelope performance and the indoor environment were unsatisfactory compared to the current legal standards, and for indoor air quality, CO2 and formaldehyde were measured to be dissatisfactory in some buildings. The energy analysis results indicated that the improvement range differed according to the renovation history for each building, resulting in differences in the energy-saving rate for each alternative. The reduction rates of primary energy consumption using energy simulation were 38.5–67.4% for office buildings and 23.7–66.3% for educational research facilities, and the payback periods were 14 to 27 years for office buildings and 12 to 30 years for educational research facilities. These results are expected to contribute to the activation of green remodeling because they can be used as indicators to predict the expected construction cost, the payback period, and the expected effect required for green remodeling

    Blind Index Modulation Detection for Pilot-Free Short-Packet Communications

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    SCP-Matrix based shipyard APS design: Application to long-term production plan

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    A shipyard is an Engineer To Order (ETO) company that designs and manufactures new products when orders are placed. Various tasks are concurrently performed, thereby making process management considerably important. It is particularly important to plan and control production activities because production constitutes the largest part of the overall process. Therefore, this study focuses on the development of a production planning system based on an Advanced Planning System (APS). An APS is an integrated planning system that targets supply chain processes in accordance with the principles of hierarchical planning. In this study, a Supply Chain Planning Matrix (SCP-Matrix), which is used as a guideline for APS development, is designed through analysis of shipyard cases. Then, we define the process in detail, starting from long-term production plan as the initial application, and design and implement a long-term production planning system using a component-based development. Keywords: Advanced Planning System (APS), Supply Chain Planning Matrix (SCP-Matrix), Engineer To Order (ETO), Shipbuilding production plan, System developmen
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