Isolasi dan Karakterisasi Enzim Selulase Cairan Rumen Sapi untuk Hidrolisis Biomassa

The purposes of this study are: 1) knowing the isolation procedure cellulase enzymes contained in cow rumen fluid. 2) observing characterization of cellulase enzymes isolated from cow\u27s liquid rumen with variables pH, temperature, and incubation time. 3) exploring glucose from the enzymatic hydrolysis of cellulose biomass on rice straw, sugarcane bagasse, rice husks, paper, and corncobs. Isolation of the enzyme was observed by employing several stages, they are dialysis stage 1, centrifugation stage 1, the purification of enzymes 40%, centrifugation stage 2, and dialysis stage 2. Glucose test results obtained by the method nelson samogyi. The specific activity of the enzyme obtained divided by the enzyme activity of cellulase enzyme protein content. Protein assay reagent used with Lowry. From this study it can be concluded that: 1). Cellulase enzymes can be obtained from the isolation of the enzyme with cellulase enzyme-making stage of the cow\u27s rumen, dialysis stage 1, centrifugation stage 1, the purification of enzymes, centrifugation and dialysis stage 2. 2) Characterization of cellulase enzymes isolated from cow\u27s liquid rumen obtained pH optimum at pH 5.4 with the activity of the enzyme 311.64 µmol/ml and the specific activity of the enzyme 11.06 µmol/mg. The optimum temperature obtained maximum activity at a temperature of 39oC with the activity of the enzyme 306.35 µmol/ml and a specific activity of enzyme of 10.87 µmol/mg. The maximum incubation time of maximum activity was obtained at 60 minutes of incubation time with enzyme activity 292.90 µmol/ml and the specific activity of the enzyme 10,39 µmol/mg. 3). Test glucose levels in rice straw biomass was obtained with glucose levels 207.25 mg/ml. Sugarcane bagasse with glucose levels 193.64 mg/ml. Rice husk with glucose levels 202.96 mg/ml. Paper with glucose levels 197.64 mg/ml. Corncobs with glucose levels 230.77 mg/ml

Sintesis Slow Release Fertilizer Berbasis Polimer Superabsorben Pengemban Pupuk Phonska

Tujuan dari penelitian ini adalah Mengetahui teknik pembuatan superabsorben berbasis selulosa dari Serbuk gergaji tercangkok sama akrilat pengemban pupuk Phonska. Mengetahui kualitas produk material superabsorben berbasis selulosa dari limbah serbuk gergaji tercangkok sama akrilat dalam mengemban pupuk Phonska . mengetahui kemampuan superabsorben dalam melepas pupuk phonska didalamnya. Penelitian ini menggunakan pengikat silang N,N Metilen Bisacrilamida dan selulosa yang digununakan berasal dari serbuk gergaji. Pupuk yang di masukan kedalam superabsorben adalah pupuk Phonska. pupuk yang digunakan di impreg kedalam superabsorben pada 4 keadaan yaitu sebelum saat dan sesudah penambahan MBA. Hasil penelitian yang dilakukan diperoleh hasil bahwa pupuk dapat terimpreg masuk kedalam superabsorben, tanpa mengganggu proses polimerisasi dan kualitas dari superabsorben. Hasil dari analisis menggunakan spektrofotometri inframerah menunjukan adanya –OH dan ikatan C=C pada semua superabsorben. Pada pupuk superabsorbent Phonska teridentifikasi adanya gugus gugus nitrogen . Superabsorben memiliki daya serap terhadap air yang tinggi berkisar 28 %b/b sampai 32 %b/b. Superabsorben pada penambahan pupuk sebelum penambahan MBA memiliki daya serap air paling tinggi. Pada uji biodigrebilitas superabsorben akan terdegradasi 82% selama 15 hari. Serta dapat melepas 73% kandungan pupuk didalamnya dalam 48 jam