23 research outputs found
Crackling Noise
Crackling noise arises when a system responds to changing external conditions
through discrete, impulsive events spanning a broad range of sizes. A wide
variety of physical systems exhibiting crackling noise have been studied, from
earthquakes on faults to paper crumpling. Because these systems exhibit regular
behavior over many decades of sizes, their behavior is likely independent of
microscopic and macroscopic details, and progress can be made by the use of
very simple models. The fact that simple models and real systems can share the
same behavior on a wide range of scales is called universality. We illustrate
these ideas using results for our model of crackling noise in magnets,
explaining the use of the renormalization group and scaling collapses. This
field is still developing: we describe a number of continuing challenges
An Overview of Three Promising Mechanical, Optical, and Biochemical Engineering Approaches to Improve Selective Photothermolysis of Refractory Port Wine Stains
During the last three decades, several laser systems, ancillary technologies, and treatment modalities have been developed for the treatment of port wine stains (PWSs). However, approximately half of the PWS patient population responds suboptimally to laser treatment. Consequently, novel treatment modalities and therapeutic techniques/strategies are required to improve PWS treatment efficacy. This overview therefore focuses on three distinct experimental approaches for the optimization of PWS laser treatment. The approaches are addressed from the perspective of mechanical engineering (the use of local hypobaric pressure to induce vasodilation in the laser-irradiated dermal microcirculation), optical engineering (laser-speckle imaging of post-treatment flow in laser-treated PWS skin), and biochemical engineering (light- and heat-activatable liposomal drug delivery systems to enhance the extent of post-irradiation vascular occlusion)
Aberrant expression of costimulatory molecules in splenocytes of the mevalonate kinase‐deficient mouse model of human hyper‐IgD syndrome (HIDS)
Objective
We sought to determine the activation status and proliferative capacities of splenic lymphocyte populations from a mevalonate kinase‐deficient mouse model of hyper‐IgD syndrome (HIDS). We previously reported that murine mevalonate kinase gene ablation was embryonic lethal for homozygous mutants while heterozygotes (Mvk+/−) demonstrated several phenotypic features of human HIDS including increased serum levels of IgD, IgA, and TNFα, temperature dysregulation, hematological abnormalities, and splenomegaly.
Methods and results
Flow cytometric analysis of cell surface activation markers on T and B lymphocytes, and macrophage populations, demonstrated aberrant expression of B7 glycoproteins in all splenic cell types studied. Differences in expression levels between Mvk+/− and Mvk+/+ littermate controls were observed in both the basal state (unstimulated) and after Concanavalin A (Con‐A) stimulation in vitro of whole splenocyte cultures. In Mvk+/− CD4 and CD8 T cells, alterations in expression of CD25, CD80, CD152, and CD28 were observed. Mvk+/− splenic macrophages expressed altered levels of CD80, CD86, CD40, and CD11c while Mvk+/− B lymphocytes had differential expression of CD40, CD80, and CD86. Mvk+/− splenocyte subpopulations also exhibited altered proliferative capacities in response to in vitro stimulation.
Conclusion
We postulate that imbalances in the expression of cell surface proteins necessary for activation, proliferation, and regulation of the intensity and duration of an immune response may result in defective T cell activation, proliferation, and effector functions in our model and potentially in human HIDS