Haplotype frequencies of 17 Y-chromosomal short tandem repeat loci from the Cukurova region of Turkey

Aim To investigate the distribution of 17 Y-short tandem repeat (STR) loci in the population of the Cukurova region of Turkey. Methods In the period between 2009 and 2010, we investigated the distribution of 17 Y-STRs in a sample of 249 unrelated healthy men from the Cukurova region of Turkey. Genomic DNA was extracted with InstaGene matrix and Y-STRs were determined using the AmpFISTR Yfiler PCR amplification kit. Gene and haplotype diversity values were estimated using the Arlequin software. To compare our data to other populations, population pairwise genetic distances and associated probability values were calculated using the Y Chromosome Haplotype Reference Database Web site software. Results At 17 Y-STR loci we detected 148 alleles. The lowest gene diversity in this region was 0.51 for DYS391 and the highest 0.95 for DYS385a/b. Haplotype diversity was 0.9997 ± 0.0004. We compared our data with haplotype data of other Turkish populations and no significant differences were found, except with Ankara population (Φst = 0.025, P = 0.018). Comparisons were also made with the neighboring populations using analysis of molecular variance of the Y-STR loci genetic structure and our population was nearest to Lenkoran-Azerbaijani (Φst = 0.012, P = 0.068) and Iranian Ahvaz population (Φst = 0.007, P = 0.173), followed by Greek (Φst = 0.026, P = 0.000) and Russian (Φst = 0.048, P = 0.000) population. Other countries like Portugal, Spain, Italy, Egypt, Israel (Palestinian Authority Area), and Taiwan showed a high genetic distance from our population. Conclusion Our study showed that Y-STR polymorphisms were a powerful discrimination tool for routine forensic applications and could be used in genealogical investigations

Evaluating the teratogenicity of the selective beta 3-adrenoceptor agonist, CL 316.243 hydrate by employing FETAX (frog embryo teratogenesis assay)

WOS: 000395010200002PubMed ID: 27063898In this study, the frog embryo teratogenesis assay (FETAX - Xenopus) technique was employed to evaluate the potential teratogenicity of the selective ss-adrenoceptor (AR) agonist, CL 316.243. In this context, CL 316.243 was applied to the South African clawed frog (Xenopus laevis) embryos. The media containing the CL 316.24-exposed embryos were monitored and changed/replaced once every 24hours. Using FETAX, we determined the minimum concentrations to inhibit growth (MCIG) for CL 316.243. The 96-hour no observable adverse effect concentration (NOAEC), the 96-hour lowest observable adverse effect concentration (LOAEC), the 96-hour EC50 (malformation) and the 96-hour LC50 (lethal concentration) for mortality and malformation could not be determined because the used concentrations did not affect viability or the presence of abnormalities. On the other hand, the MCIG of CL 316.243 was determined as 1mg/L. Our results demonstrated that CL 316.243 administration was associated with no of teratogenic and toxic effects. However, from first concentration we used (1 to 5mg/L) length of embryos reduced significantly (p<0.001) when compared to control of Xenopus embryos. Further studies should be conducted with different concentrations in order to investigate the optimal concentrations for treating preterm labor with these substances.Scientific Research Office of Cukurova University (I.U BAP)Cukurova University [TF2011BAP39]Funding for this study was provided by the Scientific Research Office of Cukurova University (I.U BAP, Project no: A part of TF2011BAP39) to AB. The BAP had no further role in the study design; in the collection, analysis and interpretation of data; in the writing of the report; and in the decision to submit the paper for publication

The toxicity and teratogenicity of gibberellic acid (GA(3)) based on the frog embryo teratogenesis assay-Xenopus (FETAX)

WOS: 000271434700001This study investigated the developmental toxicity of a plant growth regulator (a type of pesticide) using the frog embryo teratogenesis assay-Xenopus (FETAX). Xenopus laevis embryos were exposed to 11 different concentrations of gibberellic acid (GA(3)), from stage 8 to 11, for 96 h under static renewal test conditions. The median lethal concentration (LC50), malformation (EC50), non-observed adverse effect concentration (NOAEC), and lowest observed adverse effect concentration (LOAEC) were calculated. The corresponding LC50 and EC50 values determined for GA3 exposure were 1117.5 mg/L and 658 mg/L, respectively. The TI (LC50/EC50) value calculated for GA(3) was 1.69. Different anomalies occurred in the embryos, depending on the GA(3) concentration. Based on these results, we conclude that GA(3) is toxic and teratogenic to Xenopus laevis embryos. Moreover, our results confirm that the FETAX assay can be a useful pretest for integrated biological hazard assessment of chemical agents used in agriculture.Scientific Research Office of Cukurova UniversityCukurova University [TF2004BAP5]This work was supported by the Scientific Research Office of Cukurova University (I.U BAP) (project no: TF2004BAP5).; The authors acknowledge Professor Veyis TANSI from the University of Cukurova, Faculty of Agriculture for reviewing the manuscript

The Effect of Monosodium glutamate, Lithium and GSM Like Radiofrequency Irradiation on Xenopus Embryos Using FETAX Test

WOS: 000453220100159

White matter lesions and migraine

13th Congress of the International-Headache-Society -- JUN 28-JUL 01, 2007 -- Stockholm, SWEDENWOS: 000247113800129…Int Headache So

Evaluation of the Relationship Between Homocysteine, Parathormone, Vitamin D-3, and Bone Mineral Densitometry in Recipients of Kidney Transplant

1st International Transplant Network Congress -- OCT 17-21, 2018 -- Antalya, TURKEYWOS: 000487349900044PubMed ID: 31402249Purpose. In this study, we evaluated the relationship between serum homocysteine level and proteinuria, parathyroid hormone, vitamin D, and bone mineral density in kidney transplant recipients (KTR). Materials and methods. A total of 117 stable KTR older than 18 years was followed in our outpatient clinic. Demographic data were recorded. Simultaneously biochemical parameters, including glucose, blood urea nitrogenous, creatinine, calcium, phosphorus, sodium, potassium, albumin, parathormone, vitamin D-3, homocysteine, vitamin B12, folate, and 24-hour urine protein, and bone mineral density of the femoral neck and spine by dual-energy x-ray absorptiometry (DEXA) were measured. Results. DEXA measurements were normal, osteoporotic, and osteopenic (12.3%, 36.3%, and 51.3%, respectively). There was a relationship between the serum homocysteine and usage of rapamycin (P = .05), statins (P = .057), and beta blockers (P = .01), DEXA measurements were not related with serum homocysteine levels and immunosuppressive drugs used. Serum homocysteine levels correlated negatively with blood urea nitrogen (P = .002), creatinine (P =.001), vitamin B-12 (P < .001), and a positively daily proteinuria (rho = 0.203, P = .031). There was a negative relationship between proteinuria and serum level of vitamin D. Conclusions. The bone mineral density decreased in more than 87% of our KTR. We did not find any relationship between DEXA measurements and levels of homocysteine, vitamin D, parathormone, and immunosuppressive drugs. It should be noted that some drugs used may affect serum homocysteine levels. Interestingly, there was a relationship between proteinuria and serum levels of homocysteine and vitamin D. Therefore, serum levels of homocysteine and vitamin D should be evaluated for preventing renal damage in KTR

How Effective Is the Home Exercise Program for Hands in Patients with Systemic Sclerosis: Preliminary Results from a Randomized Controlled, Single-Blind, Clinical Trial

WOS: 000411824101022

CLINICAL USE OF HIGH-FREQUENCY OSCILLATORY VENTILATION FOR ACUTE RESPIRATORY DISTRESS SYNDROME IN PAEDIATRIC PATIENTS

38th Critical Care Congress of the Society-of-Critical-Care-Medicine -- JAN 31-FEB 04, 2009 -- Nashville, TNWOS: 000261213700657…Soc Crit Care Me