MRI-based computer modeling of the heart: Clinical application in arrhythmology [КОМПЬЮТЕРНОЕ МОДЕЛИРОВАНИЕ СЕРДЦА НА ОСНОВЕ МАГНИТНО-РЕЗОНАНСНОЙ ТОМОГРАФИИ: КЛИНИЧЕСКОЕ ПРИМЕНЕНИЕ В АРИТМОЛОГИИ]

N on-invasive methods for the diagnosis of heart diseases are firmly established in the clinical practice of arrhythmologists. One of the new directions of development of radiaology methods is the creation of three-dimensional mathematical models of the heart. The use of personalized mathematical models facilitates the understanding of the electrophysiological mechanisms of arrhythmias, helps in planning and evaluating the results of various treatment methods. The article presents a review of modern Russian and foreign literature on various approaches to creating models of the heart, from the first very simple computer simulations of electrophysiology to modern ones based on magnetic resonance imaging with contrast, reflecting not only the anatomy, but also the structure of the myocardium. This review also discusses the practical application of these technologies to improve the effectiveness of cardiac resynchronization therapy, ablation of ventricular arrhythmias, and assessment of postablational injuries after the intervention. Improving the development of three-dimensional personalized heart models will have an impact on the diagnosis, treatment planning, and prevention of cardiovascular diseases. © 2021 Russian Electronic Journal of Radiology. All rights reserved

Quality criteria of specialists training within the context of competencydevelopment approach

Introduction. The on-going changes in the Russian Federation have revealed a severe lack of specialists who have expertise and experience in taking decisions under the conditions of market economy, which causes an increased need in such specialists. The solution is possible if there is continuous economic education within the system of professional education. Research Methodology. In the course of the research, a competence-development teaching package has been worked out as a methodological support of continuous economic education, which is now being practically used. The package takes into account the educational level and the educational program specialization, which facilitates an individual's participation in the real economic activity. Research results.In specialists' economic skills development, it is prudent to use both traditional and innovative educational methods and technologies, which will enable to integrate pedagogical science and practice of continuous economic education and consider the succession of professional education levels and sublevels. The diversity of educational techniques will allow a teacher to realize educational goals and objectives. Discussion. The level of students' economic competencesdevelopment was measured in accordance with three criteria: the cognitive criterion, personal motivational criterion and creative activity criterion. Each of the criteria reveals through a system of empirical indexes which reflect the degree to which a particular component is developed. Conclusion. Continuous economic education is an obligatory part of continuous professional education, which is focused on developing a competitive, economically competent specialist, who is in demand in the labor market. © 2018

Quality criteria of specialists training within the context of competencydevelopment approach

Introduction. The on-going changes in the Russian Federation have revealed a severe lack of specialists who have expertise and experience in taking decisions under the conditions of market economy, which causes an increased need in such specialists. The solution is possible if there is continuous economic education within the system of professional education. Research Methodology. In the course of the research, a competence-development teaching package has been worked out as a methodological support of continuous economic education, which is now being practically used. The package takes into account the educational level and the educational program specialization, which facilitates an individual's participation in the real economic activity. Research results.In specialists' economic skills development, it is prudent to use both traditional and innovative educational methods and technologies, which will enable to integrate pedagogical science and practice of continuous economic education and consider the succession of professional education levels and sublevels. The diversity of educational techniques will allow a teacher to realize educational goals and objectives. Discussion. The level of students' economic competencesdevelopment was measured in accordance with three criteria: the cognitive criterion, personal motivational criterion and creative activity criterion. Each of the criteria reveals through a system of empirical indexes which reflect the degree to which a particular component is developed. Conclusion. Continuous economic education is an obligatory part of continuous professional education, which is focused on developing a competitive, economically competent specialist, who is in demand in the labor market. © 2018

Serological Analysis of Immunogenic Properties of Recombinant Meningococcus IgA1 Protease-Based Proteins

Using the genome sequence of IgA1 protease of N. meningitidis of serogroup B, four recombinant proteins of different structure and molecular weight were constructed. These proteins were equal in inducing the formation of specific antibodies to IgA1 protease and had protective properties against meningococci. In the sera of immunized mice, anti-IgA1 protease antibodies were detected by whole-cell ELISA, which indicated the presence of IgA1 protease on the surface of these bacteria. We hypothesized that the protective properties of IgA1 protease-based antigens and IgA1 protease analogs could be realized not only via impairment of bacterium adhesion to the mucosa, but also via suppression of this pathogen in the organism. The presented findings seem promising for using these proteins as the basis for anti-meningococcus vaccine. © 2016, Springer Science+Business Media New York

Recombinant IgA1 protease from N. meningitidis. obtaining and properties

On the base of nucleotide sequence, coding IgA1 protease from Neisseria meningitidis serogroup B, strain ÌÑ58, which was determined from data base http://www.ncbi.nlm.nih.gov/Genbank), recombinant plasmide DNA was created comprising nucleotide sequence of IgA1 protease, strain H44/76, providing IgA1 protease expression in the host-cell (pBIGAPS1). The method of expression, isolation, purification and refolding of recombinant enzyme was developed. IgA1 protease exhibits high specificity and cleaves only IgA1, but not IgG. Immunogenic and protective properties of IgA1 protease to meningococcus of serogroup À, Â and Ñ were shown. Obtained enzyme can be considered as perspective polyvaccine candidate for prophylaxis against meningococcus infection, induced by bacteria N. meningitidis and especially against serogroup B

Serological Analysis of Immunogenic Properties of Recombinant Meningococcus IgA1 Protease-Based Proteins

Using the genome sequence of IgA1 protease of N. meningitidis of serogroup B, four recombinant proteins of different structure and molecular weight were constructed. These proteins were equal in inducing the formation of specific antibodies to IgA1 protease and had protective properties against meningococci. In the sera of immunized mice, anti-IgA1 protease antibodies were detected by whole-cell ELISA, which indicated the presence of IgA1 protease on the surface of these bacteria. We hypothesized that the protective properties of IgA1 protease-based antigens and IgA1 protease analogs could be realized not only via impairment of bacterium adhesion to the mucosa, but also via suppression of this pathogen in the organism. The presented findings seem promising for using these proteins as the basis for anti-meningococcus vaccine. © 2016, Springer Science+Business Media New York

Protective properties of recombinant IgA1 protease from meningococcus

The study of enzymatic and protective properties of recombinant IgA1 protease in active and mutant form has shown that the active form of IgA1 protease exhibited species- and type-specificity for mouse and human immunoglobulins. A mutant form, lacking enzymatic activity, had protective properties against meningococcal infection, induced by meningococcus serogroup A, B and C; it protected mice from lethal infection by live virulent cultures of heterologous serogroups of meningococcus. The results obtained in this study suggest that IgA1 protease may be considered as a perspective preparation at the stages of development of a polyvalent vaccine for protection of human against meningococcal infections of various etiology. © 2013 Pleiades Publishing, Ltd

Recombinant IgA1 protease from N. meningitidis. obtaining and properties

On the base of nucleotide sequence, coding IgA1 protease from Neisseria meningitidis serogroup B, strain ÌÑ58, which was determined from data base http://www.ncbi.nlm.nih.gov/Genbank), recombinant plasmide DNA was created comprising nucleotide sequence of IgA1 protease, strain H44/76, providing IgA1 protease expression in the host-cell (pBIGAPS1). The method of expression, isolation, purification and refolding of recombinant enzyme was developed. IgA1 protease exhibits high specificity and cleaves only IgA1, but not IgG. Immunogenic and protective properties of IgA1 protease to meningococcus of serogroup À, Â and Ñ were shown. Obtained enzyme can be considered as perspective polyvaccine candidate for prophylaxis against meningococcus infection, induced by bacteria N. meningitidis and especially against serogroup B