7 research outputs found
Number of specimens from EVD suspected cases in Sierra Leone tested daily (total blood and buccal swabs) by SA FEDL during the first weeks of operation, 25 August—30 Sept 2014.
<p>Column = Number of specimens tested daily; Dotted line = Trend line of a number of specimens tested; Solid line = Maximum testing capacity of 58 specimens per day.</p
South African Ebola diagnostic response in Sierra Leone: A modular high biosafety field laboratory - Fig 3
<p>(<b>A</b>) Operators dressed in BSL3 PPE processing clinical specimens from EVD suspected cases in a glovebox located within the IsoArk biocontainment negative pressure chamber, (<b>B</b>) Blood tubes in centrifuge adapters with safety caps passed into the main chamber of the glovebox for “hot” inactivation and aliquoting for long-term storage.</p
Layout of the SA FEDL in Freetown-Lakka, Sierra Leone with emergency generator and wiring to allow for rapid switch to generator mode in case of power failure.
<p>(<b>A</b>) Biocontainment negative pressure chamber (IsoArk), (<b>B</b>) Room housing biocontainment negative pressure chamber, (<b>C</b>) Donning room, (<b>D</b>) Doffing room, (<b>E</b>) Laboratory airlock area, (<b>F</b>) PCR amplification room, (<b>G</b>) PCR master mix room, (<b>H</b>) Specimens and reagents storage area, (<b>I</b>) RNA extraction room, (<b>J</b>) Facility entrance, (<b>K</b>) Toilet, (<b>L</b>) Office 1, (<b>M</b>) Office 2, (<b>N</b>) Office 3. Petrol generator (5.5 kVa) placement indicated by the red rectangle, distribution of extension cords are indicated with red lines, and emergency connection points by red stars.</p
Results of EBOV L-gene RT-PCR in sera subjected to RNA manual (black dots) and automated (circles) extraction.
<p>Results of EBOV L-gene RT-PCR in sera subjected to RNA manual (black dots) and automated (circles) extraction.</p
Ebola virus polymerase gene TaqMan real-time RT-PCR results in buccal swabs.
<p>Ebola virus polymerase gene TaqMan real-time RT-PCR results in buccal swabs.</p
Ebola virus polymerase gene TaqMan real-time RT-PCR results in blood specimens.
<p>Ebola virus polymerase gene TaqMan real-time RT-PCR results in blood specimens.</p
Operators dressed in BSL3 PPE (scrubs, Tyvek suits, surgical gown, double pair surgical gloves, gumboots, overshoes and PAPR with full face hood) entering the IsoArk main chamber through the airlock.
<p>Operators dressed in BSL3 PPE (scrubs, Tyvek suits, surgical gown, double pair surgical gloves, gumboots, overshoes and PAPR with full face hood) entering the IsoArk main chamber through the airlock.</p