Investigation of Antiproliferative, Apoptotic and Antioxidant Effects of Oleuropein and Vitamin D on Breast Cancer Cell Lines (MCF-7)

Breast cancer is the most common female death in women. Different studies are being done for treatment. The aim of this study was to investigate the antiproliferative, apoptotic and antioxidant effects of oleuropein and vitamin D both individually and in combination. Apoptosis is a programmed cell death pattern with genetic regulation that requires energy, allowing cells to be safely removed from the environment after performing their specified biological task. The olive tree has important biological properties and is rich in phenolic substances. These phenolic substances are mainly oleuropein. Oleuropein has many pharmacological effects such as antioxidant, antimicrobial, antiviral, anticancer, antiinflammatory, antiageing. Vitamin D is a hormone the kidneys produce that controls blood calcium concentration and impacts the immune system

Lightless catalytic layered chitosan coating film using doped TiO2@metal ions nanoparticles for highly efficient dye degradation in aqueous media and disinfection applications

Chitosan-based materials are widely used in various industries due to their environmental friendliness (biodegradable and non-toxicity) and other antimicrobial properties. Improving the chitosan capacities using additional components such as titanium dioxide (TiO2) and other nanoparticles proves beneficial. Here, we propose the combination of chitosan-TiO2 nanocomposites (ChiTiO) with various metal ions (silver, zinc, copper, and iron), forming novel chitosan-based films to improve the capacities of the material further. The ChiTiO-ion metals nanocomposites and films were synthesized and characterized using dynamic light scattering (DLS), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), ultraviolet-visible spectroscopy (UV-Vis), Fourier transforms infrared spectroscopy (FT-IR), X-ray fluorescence (XRF), and cyclic voltammetry techniques. The biological activities of the metal-doped formulations showed increased antioxidant activities led by ChiTiO@Ag. Additionally, the methylene blue (MB) dye degradation capabilities were dominated by ChiTiO@Cu. The material showed a higher specificity towards MB than other dyes (methyl orange and bromophenol blue). The photocatalytic degradation at high temperatures (60 degrees C) was faster than at ambient (25 degrees C) and cold (4 degrees C) temperatures. The reusability of the film showed sustained photocatalytic capacities even after three cycles with a low loss of 16.7%. The antimicrobial activities of the films were tested against three pathogens (S. aureus, C. albicans, and P. aeruginosa) under different light conditions. Data showed ChiTiO@Ag with a concentration of 0.5 g composite having the best activity compared with the other materials. The most important finding is the ability of the proposed films to perform their activities without the need for any light activation. Lastly, ChiTiO@ion metals provide promising applications as ambient light packaging materials, coating materials, and photocatalysts.Republic of Turkey, Ministry of Development [2016K121190]The authors thank the Central Research Test and Analysis Laboratory Application and Research Center (EGE-MATAL) for their infrastructures that allowed the characterizations of the materials prepared in the current work and acknowledge the Republic of Turkey, Ministry of Development for providing the support for the infrastructure of EGE MATAL (Ege University/Izmir) via 2016K121190 grant. Dr. Serap Evran and Dr. Umut Mengulluoglu from the Department of Biochemistry (EgeUniversity/Izmir) are thanked for their technical support

Investigation of in vitro efficiency of magnetic nanoparticle-conjugated I-125-uracil glucuronides in adenocarcinoma cells

WOS: 000295609700029Modification of the magnetic properties of a drug can be used to direct the drug to the desired site, enhancing its therapeutic effectiveness and reducing side effects. In this study, surface-modified magnetic nanoparticles were immobilized with uracil glucuronide derivatives and then labeled with I-125. The morphology, structure, and composition of the magnetic particles were examined by TEM, SEM, VSM, and XRD. The particles sizes were about 50 nm. The labeling yield was 93.8% for uracil-O-glucuronide-immobilized magnetic particles and 95.0% for uracil-N-glucuronide-immobilized magnetic particles. The cell incorporation rates of N- and O-glucuronides were higher than those of uracil. The incorporation rates of uracil-, O-glucuronide-, and N-glucuronide-conjugated magnetic particles were all high. The cell incorporation rates of ligand-conjugated magnetic particles increased under a magnetic field.TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [105 S 486]; Ege UniversityEge University [06 NBE 03, 07 ILAM 01]This work has been financially supported by TUBITAK (contract no 105 S 486), Ege University Research Fund (contact no 06 NBE 03), and Ege University Research Fund (contact no 07 ILAM 01). The authors thank Adnan Menderes University Science Technology Research and Application Centre for conducting the cell culture experiments

Anadolu Üniversitesi'nin Eskişehir'e katkıları

Ankara : İhsan Doğramacı Bilkent Üniversitesi İktisadi, İdari ve Sosyal Bilimler Fakültesi, Tarih Bölümü, 2012.This work is a student project of the The Department of History, Faculty of Economics, Administrative and Social Sciences, İhsan Doğramacı Bilkent University.by İbrahim Mert Öztürk.Öztürk, İbrahim Mert. HIST 200-04ÖZTÜRK HIST 200-04/9 2011-1

The effect of systemic vancomycin on central venous catheter colonization

Central venous catheter (CVC) related infections are a major cause of morbidity and mortality. We undertook a clinical follow-up study in order to determine the value of systemic vancomycin usage before catheter insertion against catheter colonization. During the study period 70 patients with central venous catheters were prospectively studied. Patients were selected to one of two groups. All patients using vancomycin, without any other antibiotics, due to previous catheter related bacteraemia, were selected for group A. At the end of the vancomycin therapy a new CVC was inserted to a new site within an hour after the last dose (8 mg/kg). All the patients who had not used any antibiotics before and during catheter insertion were selected for Group B. None of the patients in both groups received any antibiotics for the duration that their catheters were kept in place. The catheters were removed at the end of the 7th day and cultured. In group A and group B colonization was detected with six (24.4%) and with 34 (75.6 %) CVCs, respectively. In group B colonization was detected with a significantly higher rate in comparison with group A with an odds ratio of 17.53 (95% CI: 4.150-7.054). In group A Gram positive cocci was not isolated from the catheter tip in comparison with group B in which the most commonly isolated organisms were Gram-positive cocci (n=21, 58.3%) (p<0.001). These results observed in a relatively homogeneous patient population, suggest that systemic usage of vancomycin before catheter insertion may reduce the incidence of CVC colonization, especially with Gram-positive cocci, and Gram-negative organisms dominate for the colonization