Cols bleus : hebdomadaire de la Marine française

22 mars 19971997/03/22 (N2389)-1997/03/22

Cols bleus : hebdomadaire de la Marine française

17 mai 19801980/05/17 (N1614)-1980/05/17

Additional file 6: Table S6. of The signature of liver cancer in immune cells DNA methylation

Annotated non-redundant list of 350CGs and 369 CGs that are differentially methylated between stages of HCC and healthy controls. (CSV 41 kb

Additional file 10: Table S9. of The signature of liver cancer in immune cells DNA methylation

Multifactorial ANOVA analysis of 350 CGs. No interaction detected between group (HCC) and sex and age as independent variables with CG methylation as a dependent variable. (CSV 31 kb

Additional file 3: Table S3. of The signature of liver cancer in immune cells DNA methylation

Differentially methylated sites between Stage 2 HCC and healthy controls. (CSV 1433 kb

Additional file 16: Table S15. of The signature of liver cancer in immune cells DNA methylation

Descriptive statistics for (Fig. 6a). (XLS 87 kb

Additional file 11: Table S10. of The signature of liver cancer in immune cells DNA methylation

Differentially methylated CG sites in T cell DNA between healthy controls and HCC. (CSV 1586 kb

DNA Methylation of the Serotonin Transporter Gene in Peripheral Cells and Stress-Related Changes in Hippocampal Volume: A Study in Depressed Patients and Healthy Controls

<div><p>Serotonin plays an important role in the etiology of depression. Serotonin is also crucial for brain development. For instance, animal studies have demonstrated that early disruptions in the serotonin system affect brain development and emotion regulation in later life. A plausible explanation is that environmental stressors reprogram the serotonin system through epigenetic processes by altering serotonin system gene expression. This in turn may affect brain development, including the hippocampus, a region with dense serotonergic innervations and important in stress-regulation. The aim of this study was to test whether greater DNA methylation in specific CpG sites at the serotonin transporter promoter in peripheral cells is associated with childhood trauma, depression, and smaller hippocampal volume. We were particularly interested in those CpG sites whose state of methylation in peripheral cells had previously been associated with in vivo measures of brain serotonin synthesis. Thirty-three adults with Major Depressive Disorder (MDD) (23 females) and 36 matched healthy controls (21 females) were included in the study. Depressive symptoms, childhood trauma, and high-resolution structural MRI for hippocampal volume were assessed. Site-specific serotonin transporter methylation was assessed using pyrosequencing. Childhood trauma, being male, and smaller hippocampal volume were independently associated with greater peripheral serotonin transporter methylation. Greater serotonin transporter methylation in the depressed group was observed only in SSRI-treated patients. These results suggest that serotonin transporter methylation may be involved in physiological gene-environment interaction in the development of stress-related brain alterations. The results provide some indications that site-specific serotonin transporter methylation may be a biomarker for serotonin-associated stress-related psychopathology.</p></div

Scatterplot showing the association between total childhood abuse and methylation of <i>SLC6A4</i>.

<p>There was a positive correlation between both variables indicating that more abuse is associated with higher levels of methylation.</p

Characteristics of the sample.

<p>CTQ = Childhood Trauma Questionnaire; SSRI = Selective Serotonin Reuptake Inhibitor; n.s. = not significant</p><p>Characteristics of the sample.</p