Discriminant analysis of principle components (DAPC) of multi-locus genotype data for all populations.

<p>Individual genotypes appear as circles, and black stroke circles represent the center of dispersion of each group. Populations are depicted in colors. X and Y axes are the first two principle components.</p

Genetic diversity for all <i>Centroscymnus crepidater</i> sampled locations.

<p>For mitochondrial CR sequence data: number of individuals (N), number of haplotypes (NH), haplotype diversity (<i>h</i>), nucleotide diversity (π), and standard deviation (s. d.). For microsatellite data: number of individuals (N), mean number of alleles across loci (A), allelic richness (A_R), observed (H_O) and expected (H_E) heterozygosities and heterozygote deficiency (G_IS). Significance levels are indicated (*p<0.05, ***p<0.001); values in bold indicate significance after q-value correction).</p

Pairwise mismatch distributions using the CR mtDNA data set.

<p>(A) mismatch distribution including all sequences; (B) mismatch distribution including sequences from Clade I only. Vertical bars represent observed frequencies. Upper and lower dashed lines represent the upper and lower 95% limits based on 10000 replicates in Arlequin. Solid line represents the model (expected) frequency.</p

Sampling locations of <i>Centroscymnus crepidater</i>.

<p>Haplotype frequencies and clade proportions (Clade I, in black and Clade II, in orange) based on the CR mtDNA data set are also depicted. The size of each slice is proportional to the number of individuals sharing the same haplotype. The letters A and B refer to the Atlantic and Pacific sampling locations, respectively. Sampling location codes: ROS (Rosemary Bank); ANT (Anton Dohrn Seamount); ROC (Rockall Trough); MAR (Mid-Atlantic Ridge); AZO (Azores); MAD (Madeira); GMB (Great Meteor Bank); TAS (Tasman Sea), and CHA (Chatham Rise).</p

<i>Centroscymnus crepidater</i>.

<p>Analysis of molecular variance (AMOVA) of mtDNA control region sequence data and microsatellite data between and within ocean basins. Significant values in bold (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0049196#pone-0049196-t001" target="_blank">Table 1</a> for details on sampling locations).</p

Bayesian dating analysis based on the CR mtDNA data set obtained with Multidivtime.

<p>Age estimates (in million years) of the main lineage splitting events within <i>Centroscymnus crepidater</i> are depicted. Values in square brackets represent the 95% confidence intervals. Sample codes: ROC (Rockall Trough); MAR (Mid-Atlantic Ridge); AZO (Azores); MAD (Madeira); TAS (Tasman Sea), and CHA (Chatham Rise).</p

Measures of genetic differentiation between geographic locations.

<p>(<b>A</b>) Location pairwise D values for the mitochondrial (below diagonal) and microsatellite (above diagonal) data sets. (<b>B</b>) Location pairwise Φst for the mitochondrial (below diagonal) and F_st for the microsatellite (above diagonal). The scale on the top of each figure indicates the correspondence between class intervals and colored boxes. Significant values after correction are depicted in orange.</p

Summary statistics for seven microsatellite loci of Atlantic and Pacific samples of <i>Centroscymnus crepidater:</i> number of allele counts (N_C), mean number of alleles across locations (N_A), mean allelic richness standardized to sample size N = 6, excluding Great Meteor Bank sample (A_R), effective number of alleles (E_NA), observed heterozygosity (H_O), heterozygosity within populations (H_S), corrected total heterozigosity (H’_T), and fixation index (Gis).

<p>(NA, not applicable).</p

Median Joining Network from 613 bp mtDNA COI sequences.

<p>Size of circles (haplotypes) is proportional to the relative frequencies in the sample, and colour coded according to sampling locality; abbreviations are given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0174988#pone.0174988.t001" target="_blank">Table 1</a>. Each branch indicates a single nucleotide substitution, except when noted. BOLD code represents the four sequences retrieved at Bold Systems for comparison (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0174988#sec002" target="_blank">Material and methods</a>).</p

Pairwise comparisons and tests for differentiation between localities for microsatellites (below diagonals) and mtDNA COI (above diagonals).

<p>Upper table: pairwise <i>F</i>_ST and <i>Φ</i>_ST; lower table Jost's D.</p