2 research outputs found
Self-Healing, Self-Assembled β‑Sheet Peptide–Poly(γ-glutamic acid) Hybrid Hydrogels
Self-assembled
biomaterials are an important class of materials
that can be injected and formed <i>in situ</i>. However,
they often are not able to meet the mechanical properties necessary
for many biological applications, losing mechanical properties at
low strains. We synthesized hybrid hydrogels consisting of a polyÂ(γ-glutamic
acid) polymer network physically cross-linked via grafted self-assembling
β-sheet peptides to provide non-covalent cross-linking through
β-sheet assembly, reinforced with a polymer backbone to improve
strain stability. By altering the β-sheet peptide graft density
and concentration, we can tailor the mechanical properties of the
hydrogels over an order of magnitude range of 10–200 kPa, which
is in the region of many soft tissues. Also, due to the ability of
the non-covalent β-sheet cross-links to reassemble, the hydrogels
can self-heal after being strained to failure, in most cases recovering
all of their original storage moduli. Using a combination of spectroscopic
techniques, we were able to probe the secondary structure of the materials
and verify the presence of β-sheets within the hybrid hydrogels.
Since the polymer backbone requires less than a 15% functionalization
of its repeating units with β-sheet peptides to form a hydrogel,
it can easily be modified further to incorporate specific biological
epitopes. This self-healing polymer−β-sheet peptide hybrid
hydrogel with tailorable mechanical properties is a promising platform
for future tissue-engineering scaffolds and biomedical applications
Single-Step Homogeneous Immunoassays Utilizing Epitope-Tagged Gold Nanoparticles: On the Mechanism, Feasibility, and Limitations
A single-step gold nanoparticle (AuNP)-based
immunoassay is demonstrated
in which the nanoparticle surface is tagged with short viral peptide
epitopes. Antiviral antibodies with monoclonal specificity trigger
nanoparticle aggregation yielding a colorimetric response that enables
detection of antibodies in the low-nanomolar range within a few minutes. <i>In silico</i> insights into the interactions at the epitope–gold
interface demonstrate that the conformational landscape exhibited
by the epitopes is strongly influenced by the amino acid sequence
and location of particular residues within the peptides. The conformation,
orientation, and linker chemistry of the peptides affect the immune
complex formation in nonintuitive ways that are, nevertheless, explained
by a unique sterically kinetically driven aggregation mechanism. The
rapid and specific performance of the AuNP immunoassay may have generic
potential in point of care diagnostics and other laboratory routines