Studies of the pathogenesis of slow neuroinfections using proteomic techniques

The etiology and pathogenesis of amyotrophic lateral sclerosis (ALS) are still unknown. Autoimmune mechanisms are considering to be possible causes of ALS, among several other possible mechanisms. In this paper, we describe the determination of autoantibodies against proteins of the brain motor zone and skeletal muscular system in the sera of patients suffering from ALS. Autoantibodies against carbonyl reductase 1, α-enolase, 2′,3′-phosphodiesterase of cyclic nucleotides, and pyruvate kinase 3 (isoform 2) were primarily found in the motor zone, whereas those against muscular creatine phosphokinase, myoglobine, carboanhydrase III, and troponin 1 of the fast type were identified in the skeletal muscle in the majority of patients with ALS. In addition, dynamic changes in the structure of the troponin complex were demonstrated in the tissue of skeletal muscle. The significance of the presence of autoantibodies against proteins of the brain motor zone in the sera of ALS patients is unknown. These autoantibodies most likely appeared as a secondary immunological effect of neuron damage. We can also conjecture that they accelerate the affection of muscle tissue and motoneurons. © Pleiades Publishing, Ltd. 2007

Studies of the pathogenesis of slow neuroinfections using proteomic techniques

The etiology and pathogenesis of amyotrophic lateral sclerosis (ALS) are still unknown. Autoimmune mechanisms are considering to be possible causes of ALS, among several other possible mechanisms. In this paper, we describe the determination of autoantibodies against proteins of the brain motor zone and skeletal muscular system in the sera of patients suffering from ALS. Autoantibodies against carbonyl reductase 1, α-enolase, 2′,3′-phosphodiesterase of cyclic nucleotides, and pyruvate kinase 3 (isoform 2) were primarily found in the motor zone, whereas those against muscular creatine phosphokinase, myoglobine, carboanhydrase III, and troponin 1 of the fast type were identified in the skeletal muscle in the majority of patients with ALS. In addition, dynamic changes in the structure of the troponin complex were demonstrated in the tissue of skeletal muscle. The significance of the presence of autoantibodies against proteins of the brain motor zone in the sera of ALS patients is unknown. These autoantibodies most likely appeared as a secondary immunological effect of neuron damage. We can also conjecture that they accelerate the affection of muscle tissue and motoneurons. © Pleiades Publishing, Ltd. 2007

Investigation of the complex antibiotic INA-5812

A concentrate with the antimicrobial activity has been isolated from the culture broth of Streptomyces roseoflavus INA-Ac-5812. Its further fractionation by reversed-phase HPLC has resulted in six fractions. It has been established by MALDI-TOF and ESI-MSn precision mass-spectrometry methods that the main components of the complex antibiotic are several closely related compounds, presumably of a glycopeptide nature. The fraction containing an individual component with a mass of 1845.788 Da has been characterized by UV/Vis absorbance and fluorescence spectra, amino acid analysis, and derivatization with tris(2,6-dimethoxyphenyl)methyl cation. The activity of fractions against pathogenic microbes has been studied. The results allow the supposition that the INA-5812 antibiotic complex is a glyco- or lipoglycopeptide antibiotic of a new type, which is very promising for further study. © 2016, Pleiades Publishing, Ltd

Investigation of the complex antibiotic INA-5812

A concentrate with the antimicrobial activity has been isolated from the culture broth of Streptomyces roseoflavus INA-Ac-5812. Its further fractionation by reversed-phase HPLC has resulted in six fractions. It has been established by MALDI-TOF and ESI-MSn precision mass-spectrometry methods that the main components of the complex antibiotic are several closely related compounds, presumably of a glycopeptide nature. The fraction containing an individual component with a mass of 1845.788 Da has been characterized by UV/Vis absorbance and fluorescence spectra, amino acid analysis, and derivatization with tris(2,6-dimethoxyphenyl)methyl cation. The activity of fractions against pathogenic microbes has been studied. The results allow the supposition that the INA-5812 antibiotic complex is a glyco- or lipoglycopeptide antibiotic of a new type, which is very promising for further study. © 2016, Pleiades Publishing, Ltd