Evaluation of the Oxidative Effect of Long-Term Repetitive Hyperbaric Oxygen Exposures on Different Brain Regions of Rats

Hyperbaric oxygen (HBO2) exposure affects both oxidative and antioxidant systems. This effect is positively correlated with the exposure time and duration of the treatment. The present study aims enlightening the relation of HBO2 with oxidative/antioxidant systems when administered in a prolonged and repetitive manner in brain tissues of rats. Sixty rats were divided into 6 study (n = 8 for each) and 1 control (n = 12) group. Rats in the study groups were daily exposed 90-min HBO2 sessions at 2.8 ATA for 5, 10, 15, 20, 30 and 40 days. One day after the last session, animals were sacrificed; their whole brain tissue was harvested and dissected into three different regions as the outer grey matter (cortex), the inner white matter and cerebellum. Levels of lipid peroxidation and protein oxidation and activities of superoxide dismutase and glutathione peroxidase were measured in these tissues. Malondialdehyde, carbonylated protein and glutathione peroxidase levels were found to be insignificantly increased at different time-points in the cerebral cortex, inner white matter and cerebellum, respectively. These comparable results provide evidence for the safety of HBO treatments and/or successful adaptive mechanisms at least in the brain tissue of rats, even when administered for longer periods

Comparison of the effects of hyperbaric oxygen and normobaric oxygen on sepsis in rats

Introduction: Several studies have been done on sepsis and many therapeutic agents have been developed. All agents were tested on animals prior to trials in humans. In this study, our aim was to investigate the healing effects of hyperbaric oxygen (HBO) and normobaric oxygen (NBO) due to pro-inflammatory cytokines and oxidative stress parameters, and the advantages of each other in an experimental model of sepsis. Material and methods: The rats were randomized into four groups: (1) Sham group (n = 10), intraperitoneal salineinjected group; (2) Control group (n = 10), which were only treated with CEF after induction of sepsis; (3) HBO group (n = 10), treated with HBO after sepsis induction; (4) NBO group (n= 10), treated with NBO after sepsis induction. In all groups, serum TNF- and #945;, as well as parameters of oxidative stress such as glutathione peroxidase, superoxide dismutase and malondialdehyde levels in the lung tissue, were measured. Results: Our study revealed that treatment with HBO and NBO significantly cured the increased oxidative stress and tissue membrane injury following E.coli induced experimental sepsis (p=0,001). Overall, the NBO and HBO treatments were similar. However, the HBO treatment was more efficient than the NBO treatment with respect to the TNF- and #945; levels (p=0,001). Conclusion: HBO or NBO should be used as an agent for the adjuvant treatment of sepsis. It can be concluded that applying HBO therapy as an adjuvant will be more useful for the patients meeting the criteria of sepsis. Advanced studies are required to understand the mechanism of treatment and to investigate the usability and efficiency. [Arch Clin Exp Surg 2016; 5(1.000): 7-12