7 research outputs found
Histopathologic Evaluation of Nonalcoholic Fatty Liver Disease in Hypothyroidism-Induced Rats
It is speculated that thyroid hormones may be involved in nonalcoholic fatty liver disease (NAFLD) pathogenesis. A literature scan, however, demonstrated conflicting results from studies investigating the relationship between hypothyroidism and NAFLD. Therefore, our study aims to evaluate NAFLD, from the histopathologic perspective, in hypothyroidism-induced rats. Wistar rats were divided into 2 groups: the experimental group consumed water containing methimazole 0.025% (MMI, Sigma, USA) for 12 weeks and the control group consumed tap water. At the end of week 12, serum glucose, ALT, AST, triglyceride, HDL, LDL, TSH, fT4, fT3, visfatin, and insulin assays were performed. Sections were stained with hematoxylin-eosin and "Oil Red-O" for histopathologic examination of the livers. In our study, we detected mild hepatosteatosis in all hypothyroidism-induced rats. There was statistically significant difference with respect to obesity between the two groups ( < 0.001). The mean fasting blood glucose was 126.25 ± 23.4 mg/dL in hypothyroidism-induced group and 102.63 ± 15.51 mg/dL in the control group, with a statistically significant difference between the groups ( = 0.032). The two groups did not differ statistically significantly with respect to visfatin levels ( > 0.05). In conclusion, we found that hypothyroidism-induced rats had mild hepatosteatosis as opposed to the control group histopathologically. Our study indicates that hypothyroidism can cause NAFLD
Histopathologic Evaluation of Nonalcoholic Fatty Liver Disease in Hypothyroidism-Induced Rats
It is speculated that thyroid hormones may be involved in nonalcoholic fatty liver disease (NAFLD) pathogenesis. A literature scan, however, demonstrated conflicting results from studies investigating the relationship between hypothyroidism and NAFLD. Therefore, our study aims to evaluate NAFLD, from the histopathologic perspective, in hypothyroidism-induced rats. Wistar rats were divided into 2 groups: the experimental group consumed water containing methimazole 0.025% (MMI, Sigma, USA) for 12 weeks and the control group consumed tap water. At the end of week 12, serum glucose, ALT, AST, triglyceride, HDL, LDL, TSH, fT4, fT3, visfatin, and insulin assays were performed. Sections were stained with hematoxylin-eosin and “Oil Red-O” for histopathologic examination of the livers. In our study, we detected mild hepatosteatosis in all hypothyroidism-induced rats. There was statistically significant difference with respect to obesity between the two groups (p<0.001). The mean fasting blood glucose was 126.25 ± 23.4 mg/dL in hypothyroidism-induced group and 102.63 ± 15.51 mg/dL in the control group, with a statistically significant difference between the groups (p=0.032). The two groups did not differ statistically significantly with respect to visfatin levels (p>0.05). In conclusion, we found that hypothyroidism-induced rats had mild hepatosteatosis as opposed to the control group histopathologically. Our study indicates that hypothyroidism can cause NAFLD
Effect of differently coloured clothes on fear and stress responses, some meat quality traits and performance in broilers
This study was conducted to investigate the effects of regular visual contact by the stockperson wearing clothes of different colour on fear and stress reactions, some meat quality traits and performance parameters in broiler chickens. A total of 192 one day-old male broiler chicks (Ross 308) were randomly assigned to four different colour clothing groups (red, blue, green and gray), and two visual human contact groups (60 and 300 s durations) were designed. From day 15, the chickens were exposed to regular visual contacts with the stockperson, who wore different colour clothings every day. Tonic immobility (TI) test revealed that visual contact duration increase (300 s) has a positive effect on fear in broilers. The heterophil lymphocyte (H/L) ratio was the highest (0.89) in the green clothing group, but the lowest (0.45) in red clothing group (P<0.001). From meat quality traits in breast muscle (P<0.01) it was determined that clothing colour has statistically significant effects on lightness value. Final body weights were higher in blue coloured clothing group (2593.82 g) than the green one (2348.42). The results demonstrate that regular visual human contact has no effect on parameters such as H/L ratio, some blood parameters, meat quality, TI duration and performance traits, while colour of clothes has a significant effect on H/L ratio, lightness colour value, and body weight in broiler chickens
Comparative Analysis of Serum Mineral Levels and Parasite Load inGoats Naturally Infected with Gastrointestinal Nematodes
Effect of photoperiod length and light intensity on some welfare criteria, carcass, and meat quality characteristics in broilers
ABSTRACT The objective of this study was to investigate the effects of photoperiod length and light intensity on leg and eye health, tonic immobility, some blood parameters, carcass, and meat quality characteristics in broilers. A total of 272 one-day-old male broiler chicks (Ross 308) were randomly assigned to four treatment groups based on the photoperiod length (23L:1D or increasing duration of light) and light intensity (20 lux vs. dim light) with four replicates. In this study, photoperiod lenght had no effect on incidence of foot pad and hock burn. On the other hand, the effect of photoperiod lenght had significant influence on the gait score. The incidences of gait score (3 + 4 + 5) in bright and dim light groups was found as 21.4 and 41.0% in broilers, respectively. In addition, the effect of light intensity had statistical significance on gait score. The tonic immobility duration in 20 lux and dim light groups were 271.53 and 226.78 s, respectively, and tonic immobility duration was unaffected by light intensity. All the blood parameters, except for triglyceride, were not affected by light intensity. The dim light had a negative effect on broiler welfare as demonstrated by increased eye weight and dimensions. Cold carcass yield and whole breast and wing yields were lower in the dim light group than in 20 lux light intensity. The broilers kept with dim light had lower breast meat ultimate pH (6.19) and L* values (54.30) than those reared with 20 lux. These findings have a lot of implications on the use of increasing photoperiod and bright light to improve leg an eye health benefits for the broiler welfare in broilers