4 research outputs found
Oxysterol Binding Protein Induces Upregulation of SREBP-1c and Enhances Hepatic Lipogenesis
Gene Expression in Macrophage-Rich Inflammatory Cell Infiltrates in Human Atherosclerotic Lesions as Studied by Laser Microdissection and DNA Array
In Vitro Targeting of Avidin-Expressing Glioma Cells with Biotinylated Persistent Luminescence Nanoparticles
Far red emitting persistent luminescence nanoparticles
(PLNP) were
synthesized and functionalized with biotin to study their targeting
ability toward biotin-binding proteins. First, the interaction of
biotin-decorated PLNP with streptavidin, immobilized on a plate, was
shown to be highly dependent on the presence of a PEG spacer between
the surface of the nanoparticles and the biotin ligand. Second, interaction
between biotin-PEG-PLNP and free neutravidin in solution was confirmed
by fluorescence microscopy. Finally, in vitro binding study on BT4C
cells expressing lodavin fusion protein, bearing the extracellular
avidin moiety, showed that such biotin-covered PLNP could successfully
be targeted to malignant glioma cells through a specific biotin–avidin
interaction. The influence of nanoparticle core diameter, incubation
time, and PLNP concentration on the efficiency of targeting is discussed
Efficient Pro-survival/angiogenic miRNA Delivery by an MRI-Detectable Nanomaterial
Herein, we report the use of biodegradable nanoparticles (NPs) containing perfluoro-1,5-crown ether (PFCE), a fluorine-based compound (NP170-PFCE) with the capacity to track cells <i>in vivo</i> by magnetic ressonance imaging (MRI) and efficiently release miRNA. NP170-PFCE complexed with miRNAs accumulate whitin the cell’s endolysosomal compartment and interact with higher frequency with argonaute2 (Ago2) and GW182 proteins, which are involved in the biological action of miRNAs, than commercial complexes formed by commercial reagents and miRNA, which in turn accumulate in the cell cytoplasm. The release of miRNA132 (miR132) from the NPs increased 3-fold the survival of endothelial cells (ECs) transplanted <i>in vivo</i> and 3.5-fold the blood perfusion in ischemic limbs relatively to control