Effect of high intensity interval training on serum adiponectin and motor proficiency in student boys with attention deficit hyperactivity disorder

زمینه و هدف: سطوح غیرطبیعی آدیپونکتین سرم نه تنها در چاقی، دیابت نوع دو و مقاومت به انسولین، بلکه در اختلالات روانپزشکی نیز مشاهده شده است. اختلال بیش فعالی و نقص توجه (ADHD) یکی از شایع ترین اختلالات روانپزشکی کودکان و نوجوانان است. هدف تحقیق حاضر، بررسی تأثیر 6 هفته تمرین تناوبی پرشدت بر سطوح آدیپونکتین سرم و بهبود تبحر حرکتی در پسران نوجوان مبتلا به اختلال عدم توجه/ بیش فعالی بود. روش بررسی: در این پژوهش نیمه تجربی، تعداد 20 پسر نوجوان (میانگین سنی 3/5±7/12 سال) مبتلا به اختلال بیش فعالی/ نقص توجه به صورت تصادفی به دو گروه تمرین تناوبی پرشدت و کنترل تقسیم شدند. برنامه تمرینی 6 هفته ای شامل 3 جلسه در هفته، دوی 20 متر با تعداد تکرارهای 4 نوبت در هفته های اول و دوم؛ تعداد تکرارهای 5 نوبت در هفته های سوم و چهارم و تعداد تکرارهای 6 نوبت در هفته های پنجم و ششم بود. در ابتدا و پایان هفته ششم، سطح آدیپونکتین سرم و میانگین نمره تبحر حرکتی مورد ارزیابی قرار گرفت. برای ارزیابی اثربخشی تمرین از آزمون تی مستقل استفاده گردید. یافته ها: یافته های تحلیل آماری نشان داد که اجرای تمرین تناوبی پرشدت، سطوح آدپونکتین سرم (0040/=P) و میانگین نمره تبحر حرکتی (0/001P=) را به طور معنی داری در گروه تجربی افزایش داد. نتیجه گیری: به طور کلی، مطالعه حاضر نشان داد که فعالیت تناوبی پرشدت می تواند به عنوان یک روش موثر، تأثیر مثبتی بر کاهش وزن بدن، افزایش سطح آدیپونکتین سرم و بهبود مهارت حرکتی نوجوانان مبتلا به ADHD داشته باشد

Cytokine gene expression in newly diagnosed multiple sclerosis patients

Multiple Sclerosis (MS) is characterized by multiple areas of inflammation, demyelination and neurodegeneration. Infiltrating Th1 CD4+ T cells secrete proinflammatory cytokines. They stimulate the release of some cytokines, expression of adhesion molecules and these cytokines may cause damage to the myelin sheath and axons. In this study, we analyzed plasma levels and gene expressions of five important cytokines in the new diagnosed MS Patients by ELISA and Real time PCR. PCR amplifications were performed to determine the IL-17, IL-23, IL-10, IL-27 and TGF-β mRNA expression levels using the SYBR Green PCR Kit. Our results showed significant decrease in IL-10, IL-27 and TGF-β but there was no significant difference in the IL-17 and IL-23 between patients and healthy controls. Altogether, our results indicated that dysregulation of cytokines, mainly increased expression of pro-inflammatory cytokines and decreased expression of inhibitory cytokines occurred in MS patients. This study may shed light to the probable role of these cytokines in neurodegeneration mechanism and current or future use of cytokines in managing and treatment of multiple sclerosis

Identification and cloning of putative water clarification genes of Moringa peregrina (Forssk.) Fiori in E. coli Xl 1 blue cells

Background: Water purification processes include the use of chemical compounds despite the concern that they may induce diseases. An ecological solution to this dilemma can come from the use of plant seeds for this purpose. Moringa peregrina (Forssk.) Fiori seeds have water clarification ability. Therefore, the aim of this work was to look for certain water clarification genes in M. peregrina. Materials and Methods: After preparation of M. peregrina callus, mRNA was extracted from these cells. After application of reverse transcriptase, the obtained cDNA (s) were used for PCR amplification of the desired genes using primers based on MO 2.1 gene of Moringa oleifera. DNA amplification products were cloned in E. coli Xl 1 blue cells and DNA sequences were compared with Mo 1,2 gene in M. oleifera. Results: We obtained 3 PCR products (approximately 200, 300, and 400 bps). Conclusion: After comparison of the sequences of 300bp band obtained from M. peregrina with Mo 1,2 gene in M. oleifera, it seems that 300bp band is a good candidate to investigate regarding its potential flocculent activity

Cytokeratin7 expression in gastric and colorectal adenocarcinoma: Correlation with prognostic factors

 Background: Gastric and colorectal adenocarcinoma are the second and the fifth most common cancers in Iran, respectively. Expression of cytokeratin 7 (CK7) is established in most malignancies including gastric and colorectal adenocarcinoma. Demonstration of Ck7 could be related to prognostic factors and help to the better management of the patients. The objective of our study was to evaluate the CK7 expression in gastric and colorectal adenocarcinoma and its correlation with other prognostic factors.  Methods: This cross-sectional study was performed on 99 tissue blocks from patients with gastric or colorectal adenocarcinoma undergoing surgery. Tumor grade, tumor size, depth of invasion and metastasis to lymph nodes were determined. Then, the expression of CK7 was studied using immunohistochemistry staining.  Results: Expression of CK7 was 50% and 33.8% in gastric and colorectal adenocarcinoma, respectively. There was not only a significant correlation between CK7 expression and tumor size (r=0.267, P=0.009) but also histologic grade (r=0.222, P=0.028).  Conclusion: CK7 could be more expressed with the increase in tumor size and was associated with poorly differentiated gastric and colorectal adenocarcinoma. However, with these results gathered, it is highly recommended that further studies will be conducted to reveal the exact prognostic role of this factor. &nbsp

OAC Ramsay Soybean

OAC Ramsay is an indeterminate large-seeded food-grade soybean (Glycine max [L.] Merr.) cultivar with high yield potential, high seed protein and oil concentrations, and resistance to soybean cyst nematode (SCN). OAC Ramsay is developed and recommended for soybean growing areas in southwestern Ontario with 3050 or greater crop heat units, and has a relative maturity of 2.2 (MG 2.2).The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

Effect of Ethanolic and Aqueous Extracts of Purslane on Probiotic Bacteria (Lactobacillus acidophilus and Lactobacillus casei)

ABSTRACT The use of natural ingredients such as herbs had a tremendous growth in food preservation. These compounds, in addition to antimicrobial properties, have flavoring and antioxidant properties as well. Recent research suggests that certain live microorganisms may play a role in regulating the immune system and cancer prevention that of among them to the probiotic bacteria can be noted. On the other hand, probiotic products by improving the intestinal microbial flora and digestive tract, and boost the immune system have significant impact on consumers' health. This study was conducted to evaluate the effect of aqueous and ethanolic extracts of Purslane on activities of probiotic bacteria Lactobacillus casei strain T4 and Lactobacillus acidophilus strain LA-5. The purpose of this study was to evaluate the effect of Purslane on the growth of Lactobacillus bacteria hence was prepared the aqueous and ethanolic extracts of Purslane and probiotic bacteria were exposed to various concentrations (2.5, 5 and 7%)of aqueous and ethanolic extracts. The results were evaluated after 24 hours by spectrophotometry and optical density (OD) methods. The results showed that some concentrations (2.5 and 5 %) of ethanol extract of Purslane and only the 7% concentration of aqueous extracts of purslaneincrease the growth of LA-5, whereas, the aqueous extract and also ethanolic extract of purslane at certain concentrations, can reduce the growth of T4

Machine-Learning-Based Genome-Wide Association Studies for Uncovering QTL Underlying Soybean Yield and Its Components

A genome-wide association study (GWAS) is currently one of the most recommended approaches for discovering marker-trait associations (MTAs) for complex traits in plant species. Insufficient statistical power is a limiting factor, especially in narrow genetic basis species, that conventional GWAS methods are suffering from. Using sophisticated mathematical methods such as machine learning (ML) algorithms may address this issue and advance the implication of this valuable genetic method in applied plant-breeding programs. In this study, we evaluated the potential use of two ML algorithms, support-vector machine (SVR) and random forest (RF), in a GWAS and compared them with two conventional methods of mixed linear models (MLM) and fixed and random model circulating probability unification (FarmCPU), for identifying MTAs for soybean-yield components. In this study, important soybean-yield component traits, including the number of reproductive nodes (RNP), non-reproductive nodes (NRNP), total nodes (NP), and total pods (PP) per plant along with yield and maturity, were assessed using a panel of 227 soybean genotypes evaluated at two locations over two years (four environments). Using the SVR-mediated GWAS method, we were able to discover MTAs colocalized with previously reported quantitative trait loci (QTL) with potential causal effects on the target traits, supported by the functional annotation of candidate gene analyses. This study demonstrated the potential benefit of using sophisticated mathematical approaches, such as SVR, in a GWAS to complement conventional GWAS methods for identifying MTAs that can improve the efficiency of genomic-based soybean-breeding programs

Effects of nanoliposomal and pegylated nanoliposomal artemisinin in treatment of breast cancer

This study is aimed to investigate the nanoliposomal artemisinin preparation, and its implementation on breast cancer cells. Side effects have been one of the common challenges of drug usage, as well as cancer treatment. In order to reduce such effects, nanotechnology has been a great help. Nanoliposomes are provided through reverse phase evaporation. In this method, certain proportions of phosphatidylcholine, cholesterol and artemisinin were mixed together. Besides, the obtained formulation was pegylated by using polyethylene glycol 2000 in order to increase its stability and solubility. The mean diameter of non-pegylated and pegylated liposomal artemisinin was determined by Zeta sizer system. The percent of drug released from liposome was performed by dialysis. The encapsulation efficiency of both formulations was estimated by spectrophotometry method. As a result, encapsulation and drug release of nanoliposomal formulation were more than the pegylation of the same formulation. In addition, this study indicated that cytotoxicity effect of pegylated nanoliposomal artemisinin was more, in comparison with nanoliposomal artemisinin