2 research outputs found
Isolation, characterization and complete genome sequence of PhaxI: a phage of Escherichia coli O157 :H7
Bacteriophages are considered as promising biological agents for the control of infectious
diseases. Sequencing of their genomes can ascertain the absence of antibiotic resistance, toxin
or virulence genes. The anti-O157 :H7 coliphage, PhaxI, was isolated from a sewage sample in
Iran. Morphological studies by transmission electron microscopy showed that it has an
icosahedral capsid of 85–86 nm and a contractile tail of 115�15 nm. PhaxI contains dsDNA
composed of 156 628 nt with a G+C content of 44.5 mol% that encodes 209 putative proteins.
In MS analysis of phage particles, 92 structural proteins were identified. PhaxI lyses Escherichia
coli O157 :H7 in Luria-Bertani medium and milk, has an eclipse period of 20 min and a latent
period of 40 min, and has a burst size of about 420 particles per cell. PhaxI is a member of the
genus ‘Viunalikevirus’ of the family Myoviridae and is specific for E. coli O157 : H7
Recombinant Nep1 From Fusarium oxysporum as a biological agent for control of Convolvulus arvensis
Introduction: Nep1 is a natural bio-herbicide protein which has an
effective necrosis stimulant in dicotyledonous weeds. This protein was first
purified in 1995 form fermentation broth of Fusarium oxysporum. Materials and methods: In this study, the nep1gene was isolated form F.
oxysporum, after removal of signal peptide from the beginning of the gene;
final pET16b-nep1 construct was cloned and transformed into E. coli BL21
(DE3). The recombinant Nep1 was produced in
E. coli. Recombinant protein was purified, diluted and prepared for
biological assay on weed. Results: Two ml of recombinant Nep1 with 60 µg/ ml final concentrations was sprayed on Convolvulus arvensis. Our results showed necrosis and chlorosis symptoms were
started on the plant leaves after 2 days, also significant necrosis on the
leaves of C.
arvensis was
seen after
5 days. No necrosis lesion was seen in
the negative control plants that sprayed only with buffer and denatured
recombinant Nep1 in 100 ºC for 15 min. Discussion and conclusion: Our results introduced recombinant Nep1 as a biological potent agent
for biocontrol of C. arvensis