3 research outputs found
<i>Euodia daniellii</i> Hemsl. Extract and Its Active Component Hesperidin Accelerate Cutaneous Wound Healing via Activation of Wnt/β-Catenin Signaling Pathway
The activation of the Wnt/β-catenin signaling pathway plays a key role in the wound-healing process through tissue regeneration. The extract of Euodia daniellii Hemsl. (E. daniellii), a member of the Rutaceae family, activates the Wnt/β-catenin signaling pathway. However, the function of E. daniellii in wound healing has not yet been elucidated. We performed a migration assay to determine the wound-healing effect of E. daniellii extract in vitro using human keratinocytes and dermal fibroblast. In addition, a mouse acute wound model was used to investigate the cutaneous wound-healing effect of E. daniellii extract in vivo and confirm the potential mechanism. E. daniellii extract enhanced the migration of human keratinocytes and dermal fibroblasts via the activation of the Wnt/β-catenin pathway. Moreover, the E. daniellii extract increased the levels of keratin 14, PCNA, collagen I, and α-SMA, with nuclei accumulation of β-catenin in vitro. E. daniellii extract also efficiently accelerated re-epithelialization and stimulated wound healing in vivo. Furthermore, we confirmed that hesperidin, one of the components of E. daniellii, efficiently accelerated the migration of human keratinocytes and dermal fibroblasts, as well as wound healing in vivo via the activation of the Wnt/β-catenin pathway. Overall, E. daniellii extract and its active component, hesperidin, have potential to be used as therapeutic agents for wound healing
Discovery of Orally Available Runt-Related Transcription Factor 3 (RUNX3) Modulators for Anticancer Chemotherapy by Epigenetic Activation and Protein Stabilization
Recently, we identified a novel strategy
for anticancer chemotherapy
by restoring runt-related transcription factor 3 (RUNX3) levels via
lactam-based histone deacetylase (HDAC) inhibitors that stabilize
RUNX3. Described here are the synthesis, biological evaluation, and
pharmacokinetic evaluation of new synthetic small molecules based
on pyridone-based HDAC inhibitors that specifically stabilize RUNX3
by acetylation and regulate its function. Many of the newly synthesized
compounds showed favorable RUNX activities, HDAC inhibitory activities,
and inhibitory activities on the growth of human cancer cell lines.
Notably, one of these new derivatives, (<i>E</i>)-<i>N</i>-hydroxy-3-(2-oxo-1-(quinolin-2-ylmethyl)-1,2-dihydropyridin-3-yl)acrylamide
(<b>4l</b>), significantly restored RUNX3 in a dose-dependent
manner and showed high metabolic stability, a good pharmacokinetic
profile with high oral bioavailability and long half-life, and strong
antitumor activity. This study suggests that pyridone-based analogues
modulate RUNX3 activity through epigenetic regulation as well as strong
transcriptional and post-translational regulation of RUNX3 and could
be potential clinical candidates as orally available RUNX3 modulators
for the treatment of cancer