2 research outputs found
Tailoring a Tyrosine-Rich Peptide into Size- and Thickness-Controllable Nanofilms
Self-assembled
nanostructures of tyrosine-rich peptides have a
number of potential applications such as biocatalysts, organic conducting
films, and ion-selective membranes. In modulating a self-assembly
process of peptides, the interfacial force is an important factor
for kinetic control. Here, we present the formation of large-sized
and thickness-controllable nanofilms from the YYACAYY peptide sequence
(Tyr-C7mer peptide) using Langmuir–Blodgett and Langmuir–Schaefer
deposition methods. The Tyr-C7mer peptide showed typical surfactant-like
properties, which were demonstrated via the isotherm test (surface
pressure–area) by spreading the Tyr-C7mer peptide solution
onto an air/water interface. Uniform and flat peptide nanofilms were
successfully fabricated and characterized. The redox activity of densely
packed tyrosine moieties on the peptide nanofilm was also evaluated
by assembling silver nanoparticles on the nanofilm without requiring
any additives
Solid-Phase Synthesis of Peptide-Conjugated Perylene Diimide Bolaamphiphile and Its Application in Photodynamic Therapy
Here,
we describe a rapid and efficient synthetic method of peptide-conjugated
perylene diimide (P-PDI) using solid-phase peptide synthesis (SPPS).
Due to severe insolubility of perylene dianhydride (PDA) as a starting
material of perylene diimide (PDI), PDA was initially conjugated with
amino acids to obtain soluble PDI derivatives. Target peptides were
synthesized on a 2-chlorotrityl chloride resin using the SPPS method
and then conjugated with the amino acid-appended PDI. Various conditions
such as loading levels, reaction times and solvents were optimized
for introducing the peptides to both sides of the amino acid-appended
PDI. The final P-PDI was obtained with a maximum yield of 80% in 12
h. Its singlet oxygen-derived phototoxicity on cells was confirmed,
which could be applicable to photodynamic therapy