Value of the expression of Ki-67 and membrane protein glucose transporter GLUT1 in the diagnosis of neoplastic transformation of the oral mucosal epithelium

Objective: To investigate the expression of GLUT1 protein in relation to the proliferative activity of cells and the degree of neoplastic transformation of the epithelium of the oral mucosa (OM). Subjects and methods. OM biopsies from 44 patients were investigated. 14 cases (31.9%) were diagnosed with focal epithelial hyperplasia of the OM; 13 (29.5%) cases had squamous intraepithelial neoplasia (SIN) and 17 (38.6%) had squamous cell carcinoma. Ten intact MO samples were used as a control group. Tissue antigens were determined using mouse monoclonal antibodies to Ki-67 and rabbit antibodies to GLUT1. Results. A direct correlation was found between the intensity of GLUT1 expression on the oral epithelial cell membranes and the proliferative activity of cells with an increase in the phenomena of cellular atypia. Conclusion. GLUT1 may be used as a marker for the early diagnosis of neoplastic transformation of stratified squamous epithelial cells

Immunohistochemical criteria for differential diagnostics of squamous epithelium changes in pyogenic granuloma and squamous cell carcinoma of the oral mucosa [Immunogistokhimicheskie kriterii differentsial'noĭ diagnostiki ploskogo épiteliia pri piogennoĭ granuleme i ploskokletochnom rake slizistoĭ obolochki rta]

The paper presents an example of differential diagnostic studies for pyogenic granuloma and oral squamous cell carcinoma (SCC). In the described case immunohistochemistry with antibodies to Ki-67 and Gli1 was used as conventional histological procedure proved to be inconvenient for adequate diagnostics. The immunohistochemical study established increased proliferative activity of epithelial cells specific both pyogenic granuloma and oral SCC, but intensive Gli1 protein expression in membranes and cytoplasm of epithelial cells with malignant transformation allowed differentiation of these neoplasms.Представлен пример дифференциально-диагностических исследований плоского эпителия при пиогенной гранулеме и плоскоклеточном раке слизистой оболочки рта (СОР). В описанном случае ввиду сложности постановки диагноза при обычной гистологической методике был применен иммуногистохимический (ИГХ) метод с антителами к белкам Ki-67 и Gli1. В результате ИГХ-исследования установлена повышенная пролиферативная активность эпителиальных клеток, характерная как для эпителия в пиогенной гранулеме, так и для плоскоклеточного рака СОР. Интенсивная экспрессия белка Gli1 на мембранах и в цитоплазме при злокачественном перерождении эпителиальных клеток позволяет дифференцировать эти новообразования

Cytokeratin 15 as a diagnostic marker for oral epithelial malignization [Tsitokeratin 15 kak diagnosticheskiĭ marker nachala malignizatsii épiteliia slizistoĭ obolochki rta]

The paper presents an example of an immunohistochemical study of malignancy in oral epithelial hyperplasia. In the described case in view of the difficulty in determining the onset of the epithelial malignancy with the usual histological technique, an immunohistochemical method was used with antibodies to proteins Ki-67, human papillomavirus (HPV) type 16 and cytokeratin 15. The results of immunohistochemical study indicated increased proliferative activity of epithelial cells, presence of papillomavirus HPV16 in their cytoplasm and synthesis of cytokeratin 15 in all layers of the epithelium. These characteristics of squamous intraepithelial neoplasia may be a diagnostic criterion for the beginning of oral epithelial malignization.В работе представлен пример иммуногистохимического исследования случая малигнизации эпителия при очаговой эпителиальной гиперплазии слизистой оболочки рта (СОР). В описанном случае ввиду сложности определения начала малигнизации эпителия при обычной гистологической методике был применен иммуногистохимический метод с антителами к белкам Ki-67, вирусу папилломы человека 16-го типа и цитокератину 15. В результате иммуногистохимического исследования установлены повышенная пролиферативная активность эпителиальных клеток, наличие в их цитоплазме папилломавируса HPV16 и синтез цитокератина 15 в клетках всех слоев эпителия, что характерно для плоскоклеточной интраэпителиальной неоплазии и может быть диагностическим критерием начала малигнизации эпителия СОР

Value of the expression of Ki-67 and membrane protein glucose transporter GLUT1 in the diagnosis of neoplastic transformation of the oral mucosal epithelium

Objective: To investigate the expression of GLUT1 protein in relation to the proliferative activity of cells and the degree of neoplastic transformation of the epithelium of the oral mucosa (OM). Subjects and methods. OM biopsies from 44 patients were investigated. 14 cases (31.9%) were diagnosed with focal epithelial hyperplasia of the OM; 13 (29.5%) cases had squamous intraepithelial neoplasia (SIN) and 17 (38.6%) had squamous cell carcinoma. Ten intact MO samples were used as a control group. Tissue antigens were determined using mouse monoclonal antibodies to Ki-67 and rabbit antibodies to GLUT1. Results. A direct correlation was found between the intensity of GLUT1 expression on the oral epithelial cell membranes and the proliferative activity of cells with an increase in the phenomena of cellular atypia. Conclusion. GLUT1 may be used as a marker for the early diagnosis of neoplastic transformation of stratified squamous epithelial cells

Immunohistochemical criteria for differential diagnostics of squamous epithelium changes in pyogenic granuloma and squamous cell carcinoma of the oral mucosa [Immunogistokhimicheskie kriterii differentsial'noĭ diagnostiki ploskogo épiteliia pri piogennoĭ granuleme i ploskokletochnom rake slizistoĭ obolochki rta]

The paper presents an example of differential diagnostic studies for pyogenic granuloma and oral squamous cell carcinoma (SCC). In the described case immunohistochemistry with antibodies to Ki-67 and Gli1 was used as conventional histological procedure proved to be inconvenient for adequate diagnostics. The immunohistochemical study established increased proliferative activity of epithelial cells specific both pyogenic granuloma and oral SCC, but intensive Gli1 protein expression in membranes and cytoplasm of epithelial cells with malignant transformation allowed differentiation of these neoplasms.Представлен пример дифференциально-диагностических исследований плоского эпителия при пиогенной гранулеме и плоскоклеточном раке слизистой оболочки рта (СОР). В описанном случае ввиду сложности постановки диагноза при обычной гистологической методике был применен иммуногистохимический (ИГХ) метод с антителами к белкам Ki-67 и Gli1. В результате ИГХ-исследования установлена повышенная пролиферативная активность эпителиальных клеток, характерная как для эпителия в пиогенной гранулеме, так и для плоскоклеточного рака СОР. Интенсивная экспрессия белка Gli1 на мембранах и в цитоплазме при злокачественном перерождении эпителиальных клеток позволяет дифференцировать эти новообразования

The proliferative activity of tumor cells and the distribution of SOX2+ pluripotent stem cells in various histological types of ameloblastoma

Objective: to investigate the proliferative activity and distribution of pluripotent stem cells in various histological types of amelobrastoma. Materials and methods. The surgical and archival materials of 76 patients with ameloblastoma from the Central Research Institute of Dentistry and Oral Surgery, Ministry of Health of the Russian Federation, were used. Tissue antigens were determined using a rabbit monoclonal antibody against to Ki-67 and SOX2. Results. Proliferating (Ki-67) and pluripotent (SOX2) cells were identified in various histological types of ameloblastoma. A weak significant correlation was found between tumor size, pluripotent stem cells, and cellular proliferative activity. Enhanced cell proliferative activity and extensive bone tissue destruction significantly correlated with the development of recurrent ameloblastoma. Conclusion. The presence of pluripotent stem cells, the proliferative activity of ameloblastoma cells, and the extensiveness of bone tissue destruction allow basal-cell and plexiform options to be considered as the most aggressive indicators

Ki-67 and matrix metalloproteinase-9 expression in the follicular cyst, keratocystic odontogenic tumor, and ameloblastoma

An immunohistochemical study using antibodies against Ki-67 protein was conducted, which characterized the proliferative activity of cells and matrix metalloproteinase-9 (MMP-9) in follicular cyst, variants of keratocystic odontogenic tumor (with a preponderance of hyperkeratosis and parakeratosis), and ameloblastoma. The marked proliferative activity of a parabasal cell layer (28.0±8.4%) was found in the keratocystic odontogenic tumor with a preponderance of parakeratosis; the proliferative activity of the peripheral layer of ameloblastoma cells was equal to 7.0±5.6%. The maximal matrix metalloproteinase-9 expression in the keratocystic odontogenic tumor with a predominance of hyperkeratosis was 1.1 ±0.9 conventional units (CU) and that in the ameloblastoma was 1.9±1.3 CU versus 0.4±0.5 CU in the follicular cyst, keratocystic odontogenic tumor with a preponderance of hyperkeratosis. The values of Ki-67 and MMP-9 expression allow one to distinguish benign odontogenic cysts and tumors (follicular cyst and keratocystic odontogenic tumor with a predominance of hyperkeratosis) and odontogenic tumors characterized by an aggressive clinical course (keratocystic odontogenic tumor with a preponderance of parakeratosis and ameloblastoma)

Immunohistochemical diagnosis of necrotizing sialometaplasia [Immunogistokhimicheskaya diagnostika nekroticheskoi sialometaplazii]

The article presents a clinical case of an immunohistochemical study of a rare disease: necrotizing sialometaplasia in the hard palate. Due to the complexity of the differential diagnosis between necrotizing sialometaplasia and squamous cell carcinoma, an immunohistochemical method was used with antibodies to proteins Ki-67, P53, P63 and cytokeratins-7 and Immunohistochemical study established low proliferative activity of glandular cells in excretory ducts and metaplastic squamous epithelium for Ki-67 protein, significant expression of protein P63 was detected both in the ductal epithelium nuclei and in metaplastic areas and expression of the mutant protein P53 was approximately absent. Marked expression of cytokeratin-7 in the ducts cells and weak expression in necrotic acini of the glands and metaplastic epithelium was noted. It was shown that cytokeratin-15 homogeneously stains the peripheral zone of metaplastic epithelium, which is characteristic of a benign lesion. Thus, the panel of antibodies to proteins Ki-67, P53 and cytokeratins-7 and 15 allows to verify necrotizing sialometaplasia.В статье представлен клинический случай иммуногистохимического исследования редкого заболевания — некротической сиалометаплазии на твердом небе. Ввиду сложности дифференциальной диагностики между некротической сиалометаплазией и плоскоклеточным раком был применен иммуногистохимический метод с антителами к белкам Ki-67, P53, P63 и цитокератинам 7 и 15. По результатам иммуногистохимического исследования установлена низкая пролиферативная активность железистых клеток выводных протоков и метаплазированного плоского эпителия по белку Ki-67, выраженная экспрессия белка P63 выявлена как в ядрах клеток протокового эпителия, так и в участках метаплазии, экспрессия мутантного белка Р53 практически отсутствовала. Отмечалась выраженная экспрессия цитокератина-7 в клетках протоков и слабая — в некротизированных ацинусах желез и метаплазированном эпителии. Показано, что цитокератин-15 гомогенно окрашивает периферическую зону метаплазированного эпителия, что характерно для доброкачественного образования. Панель антител к белкам Ki-67, P53 и цитокератинам 7 и 15 позволяет верифицировать некротическую сиалометаплазию

Immunohistochemical study of Ki-67, PHH3, and CK15 protein expression in oral epithelial malignancy

Objective to investigate the expression of Ki-67, phosphohistone H-3 (PHH3), and cytokeratin 15 (CK15) proteins in the cells of the oral mucosa (OM) according to the degree of its malignant transformation. Material and methods. OM biopsy specimens from 69 patients diagnosed with focal epithelial hyperplasia, intraepithelial squamous cell neoplasia, cancer in situ, and squamous cell carcinoma were examined. Tissue antigens were determined using mouse Ki-67 monoclonal antibodies, rabbit PHH3 polyclonal antibodies, and mouse CK15 monoclonal antibodies. Results. There was an increase in epithelial proliferative and mitotic activities in squamous cell carcinoma and a sharp decrease in the expression of CK15 in the cytoplasm in cancer in situ and squamous cell carcinoma of the OM. Conclusion. The protein CK15 can be used for the differential diagnosis between high-grade dysplasia and OM epithelial malignancy at the stage of carcinoma in situ and squamous cell carcinoma. © A.A. IVINA, V.A. SEMKIN, Z.S. KHABADZE, I.I. BABICHENKO

Ki-67 and matrix metalloproteinase-9 expression in the follicular cyst, keratocystic odontogenic tumor, and ameloblastoma

An immunohistochemical study using antibodies against Ki-67 protein was conducted, which characterized the proliferative activity of cells and matrix metalloproteinase-9 (MMP-9) in follicular cyst, variants of keratocystic odontogenic tumor (with a preponderance of hyperkeratosis and parakeratosis), and ameloblastoma. The marked proliferative activity of a parabasal cell layer (28.0±8.4%) was found in the keratocystic odontogenic tumor with a preponderance of parakeratosis; the proliferative activity of the peripheral layer of ameloblastoma cells was equal to 7.0±5.6%. The maximal matrix metalloproteinase-9 expression in the keratocystic odontogenic tumor with a predominance of hyperkeratosis was 1.1 ±0.9 conventional units (CU) and that in the ameloblastoma was 1.9±1.3 CU versus 0.4±0.5 CU in the follicular cyst, keratocystic odontogenic tumor with a preponderance of hyperkeratosis. The values of Ki-67 and MMP-9 expression allow one to distinguish benign odontogenic cysts and tumors (follicular cyst and keratocystic odontogenic tumor with a predominance of hyperkeratosis) and odontogenic tumors characterized by an aggressive clinical course (keratocystic odontogenic tumor with a preponderance of parakeratosis and ameloblastoma)