Antibacterial and antioxidant activity of some seeds used as food

In this study, antioxidant and antibacterial activities of Brassica nigra, Linum usitatissimum, Salvia hispanica, Nigella sativa, Chenopodium quinoa seeds prepared with ethanol were determined. Antioxidant capacity has been measure by evaluating DPPH radical scavenging activity level and antibacterial activities were investigated by using the disc diffusion method. Highest antibacterial activity level has been detected for the seed of N. sativa. As a result of this study there exist no any antibacterial effect in the seeds of B. nigra and L. usitatissimum. The extract of N. sativa seed has shown an inhibition zone of 14.5 mm on Staphylococcus aureus, whereas this value has been detected to be 10.5 mm for both on the seeds of Enterococcus faecalis and Staphylococcus epidermidis. IC50 value showing the 50% scavenging value of DPPH radical has been determined to be 51 µg/mL for the seed extrafct of B. nigra as the highest one between the all seed samples searched. All seeds used in the current study which are known to be functional food have shown certain level of antioxidant activity

Antioxidant activity of the fruits of Pyracantha coccinea using ethanolic extract method

Pyracantha coccinea Roem. (scarlet firethorn) is known as a medicinal plant that can be up to 3 m in height with lots of thorns, grown generally in South and Southeast Europe and Turkey. Pyracantha coccinea Roem is being used in folk medicine since its fruits have diuretic, cardiac and tonic properties. To determine the antioxidant level of the different parts of the plants takes interest in the current researches. In this study it has been aimed to evaluate the antioxidant and antimicrobial activity levels, Fe3+ reduction power and the total phenolic amount of the ethanolic extracts of the fruits of Pryacantha coccinea using DPPH scavenging, the disc diffusion, reduction power and Folin-Ciocalteu methods, respectively. IC50 value, which represents the 50% scavenging value of DPPH radical of prepared fruit extracts using the Maceration technique, is evaluated to be 36.53 µg/mL The total phenolic content is determined to be 199.6 mgGA/100g and Fe+3 ions are reduced to a certain amount for various concentration levels (from 20 to 100 µg/mL). From the current research we have also found that the fruits of Pyracantha coccinea have no any antimicrobial activity. As a general result of the current study it has been concluded that the fruits of Pyracantha coccinea have extremely higher level of antioxidant activity depending upon the phenolic contents, showing that they can be used in various food and health applications

Allium scodoprasum subsp. Rotundum, allium staticiforme ve allium subhirsutum türlerinin antimikrobiyal ve antioksidan aktivitelerinin araştırılması

06.03.2018 tarihli ve 30352 sayılı Resmi Gazetede yayımlanan “Yükseköğretim Kanunu İle Bazı Kanun Ve Kanun Hükmünde Kararnamelerde Değişiklik Yapılması Hakkında Kanun” ile 18.06.2018 tarihli “Lisansüstü Tezlerin Elektronik Ortamda Toplanması, Düzenlenmesi ve Erişime Açılmasına İlişkin Yönerge” gereğince tam metin erişime açılmıştır.Bu çalışmada aseton ve metanol ile hazırlanan Allium scorodoprasum L. subsp. rotundum, Allium staticiforme ve Allium subhirsutum ekstraktlarının B. subtilis ATCC 6633, S. aureus ATCC 29213, E. faecalis ATCC 29212, S. typhimurium ATCC 14028, E. coli ATCC 25922, S. epidermidis ATCC 12228, P. aeruginosa ATCC 27853 ve C. albicans ATCC 1029 suşlarına karşı disk difüzyon yöntemi kullanılarak antimikrobiyal etkileri araştırılmıştır. Ayrıca Allium staticiforme ve Allium subhirsutum metanolik ekstraktlarının DPPH yöntemi kullanılarak antioksidan aktiviteleri ve Folin-Ciocalteu reaktifi ile toplam fenolikleri belirlenmiştir. Allium staticiforme ve Allium subhirsutum ekstraktlarının C. albicans üzerinde yüksek antifungal aktivite gösterdiği belirlenirken, kullanılan bakteriler üzerinde geniş spektrumlu antbakteriyel aktiviteye sahip olduğu tespit edilmiştir. Allium scorodoprasum L. subsp. rotundum ekstraktlarının antimikrobiyal aktivite göstermediği belirlenmiştir. Allium staticiforme ve Allium subhirsutum ekstraktlarının IC50 değerleri 800-1300 µg/mL arasında olduğu gözlenmiştir. Türkiye florasındaki Allium staticiforme, Allium subhirsutum bitkilerinin yeni bir antifungal kaynak olarak kullanılabileceği önerilebilirIn this study, the antibacterial activity of Allium scorodoprasum L. subsp. Allium staticiforme and Allium subhirsutum extracts (acetone, methanol) againts microbial strains B. subtilis ATCC 6633, S. aureus ATCC 29213, E. faecalis ATCC 29212, S. typhimurium ATCC 14028, E. coli ATCC 25922, S. epidermidis ATCC 12228 P. aeruginosa ATCC 27853 and C. albicans ATCC 1029 antimicrobial effects were examined by using disc diffusion method. Addition, Allium staticiforme, Allium subhirsutum methanolic extracts were determined the antioxidant activities by using DPPH method and their total phenolics were determined by FCR method. Extracts of Allium staticiforme and Allium subhirsutum were showed high antifungal activity on C. albicans. It has been determined that it has a broad-spectrum antibacteril activity on the bacteria used. It was determined that extracts of Allium scodoroprsum rotundum did not show antimicrobial activity. The IC50 values of Allium staticforme and Allium subhirsutum were between 800-1300 µg / mL. Allium subhirsutum and Allium staticiforme in the flora of Turkey can be used as a new antifungals source

Antimicrobial activities of allium staticiforme and allium subhirsutum

The antibacterial and antifungal activities of the bulb and flower of Allium statciforme and Allium subhirsutum were investigated. In addition, DPPH radical scavenging activity and total phenolic contents were determined. The results show that methanolic extracts of A. staticiforme and A. subhirsutum had antifungal activities against Candida albicans, together with a less activity level against Escherichia coli, Staphylococcus epidermidis, S. aureus, Enterecoccus faecalis, Salmonella typhimurium and Pseudomanas aeruginosa. The total phenolic contents of A. staticiforme leaf and bulb were determined as 17 and 2.4 mg of GAW100 g, respectively. The IC50 of methanolic extracts of A. staticiforme and A. subhirsutum were also determined. In conclusion, both A. staticiforme and A. subhirsutum have antifungal activities with weak antibacterial activities. These plants have DPPH radical scavenging activities

Solving X-ray protein structures without a crystal: using X-ray Free Electron Laser, the fourth generation synchrotron light sources

In the present study, the total antioxidant activity, the total phenolic content, and the antimicrobial activity of methanol extracts obtained from the female cones of Pinus brutia, Pinus nigra, Cupressus sempervirens L., Thuja orientalis L., and Cedrus libani were evaluated. The in vitro total phenolic content, the antioxidant, and the antimicrobial activities were determined using the Folin-Ciocalteu reaction, the DPPH radical scavenging assay and the disc diffusion method, respectively. The results of the present study showed that the extracts of the female cones prepared in methanol had the antimicrobial activity against the test microorganisms and the highest antimicrobial effect was observed against Staphylococcus epidermidis, Staphylococcus aureus, and Bacillus subtilis. The total phenolic constituents of the extracts were determined to be in the ranges from 69 to 220 mg GA/100 g. All extracts exhibited antioxidant activity with concentrations necessary to inhibit the activity of DPPH radical by 50% (IC50) ranging from 0.35 to 17.21 µg/mL. The results indicate that the extracts of the cones exhibit acceptable antioxidant and antimicrobial activities and suggest that these cones’ extracts may serve as a potential source of natural antioxidants and antimicrobials for food or medical purposes