Delayed wound healing in CPST mice.

<p>Normal skin sections of CPST and WT mice stained with Hematoxylin-Eosin-Safran (A) and Sirius Red (B) do not show any differences at steady state. 5 mm punch biopsies were performed on CPST and WT mice. Photographs (C) and surface (D) of the wounds at multiple time points showing delayed wound closure in CPST mice. Diametrical sections of wounds stained with Hematoxylin-Eosin depicting neo-epidermis (dashed lines) (E) and length of neo-epidermis on keratin 14-labeled sections (F) showing delayed reepidermization in CPST mice. n = 7 per group per time point. Scale bars: (A and B) 100 µm, (E) 500 µm. (D) Mann-Whitney test, (F) ANOVA, *<i>P</i><0.05; ***<i>P</i><0.001. (D and F) mean +/−SEM.</p

Calpain inhibition reduces scar formation in skin wounds.

<p>Day 10 Wounds of CPST mice show less Sirius Red staining and more epidermal adnexa regeneration compared to WT mice (A). Relative surface of Sirius Red staining in WT and CPST wounds on day 10 (B). mRNA levels of Collagen1A1 and TGFβ1 in day 3 CPST and WT wound (C and D). (A–D) n = 7 per group. Scale bars: (A) 500 µm. Mann-Whitney test, *<i>P</i><0.05. (B–D) mean +/−SEM.</p

Histological examination of a skin biopsy specimen of the plaque showed on figure 1: lymphohistiocytic hypodermal infiltrate, associated with non-caseating multinucleated giant cells granulomas (Hematoxylin-eosin Strain; original magnification ×100)

<p><b>Copyright information:</b></p><p>Taken from "Misleading pustular plaques of the lower limbs during Crohn's disease: two case reports"</p><p>http://www.jmedicalcasereports.com/content/1/1/109</p><p>Journal of Medical Case Reports 2007;1():109-109.</p><p>Published online 3 Oct 2007</p><p>PMCID:PMC2045104.</p><p></p

Impaired angiogenesis in CPST wounds.

<p>Wound sections (day 7) of CPST and WT mice were double-labeled for CD31 (red) and Lyve1 (green). Nuclei were stained with DAPI (blue). Representative photos of wound granulation tissue in WT and CPST mice are depicted in (A). Number (B) and surface (C) of blood and lymphatic vessels in the wound beds. n = 7 per group. Scale bar (A) 100 µm. Mann-Whitney test, *<i>P</i><0.05. (B and C) mean +/−SEM.</p

Impaired proliferation and granulation tissue formation in CPST wounds.

<p>Ki67 staining (red) on CPST and WT wound sections at day 7 (A) showed reduced numbers of proliferating cells in the epidermal wound edges and the granulation tissue dermal cells (B). CD45 staining (red) and counts (C and D) on day 3 wound sections showed reduced numbers of inflammatory cells in CPST wound beds (D). α-Smooth-Muscle-Actin staining (αSMA) (red) and counts (E and F) showed reduced numbers of myofibroblasts in CPST wounds. mRNA levels of αSMA in day 3 CPST and WT wounds. Representative photos of wound edges are depicted in A; epidermis is delineated in dashed lines. Representative photos of wound granulation tissue are depicted in (C) and (E). Nuclei were stained with DAPI (blue). n = 7 per group. Scale bars: (A) 100 µm, (C and E) 50 µm. Mann-Whitney test, *<i>P</i><0.05, **<i>P</i><0.01. (B, D, F and G) mean +/−SEM.</p

Photomicrographs showing male cells with X (red) and Y (green) chromosome signals within breast carcinoma after fluorescence hybridization (FISH)

Male cells after FISH (arrows). Original magnifications: 630× for panel a and 1,000× for panel b. A cluster of cytokeratin-positive cells: panel c shows male cells after FISH in a cluster of maternal cells (arrow), and panel d shows cytokeratin-positive male cell after FISH and immunolabelling (arrow). Original magnification: 1,000× for both panels. A cluster of vimentin-positive cells: panel e shows a male cell after FISH in a cluster of maternal cells (arrow), and panel f shows a vimentin-positive male cell after immunolabelling (arrow). Original magnification: 1,000× for both panels.<p><b>Copyright information:</b></p><p>Taken from "Breast cancer stroma frequently recruits fetal derived cells during pregnancy"</p><p>http://breast-cancer-research.com/content/10/1/R14</p><p>Breast Cancer Research : BCR 2008;10(1):R14-R14.</p><p>Published online 13 Feb 2008</p><p>PMCID:PMC2374970.</p><p></p

Frequency of foetal cells per million of maternal cells in breast carcinoma and in control group

<p><b>Copyright information:</b></p><p>Taken from "Breast cancer stroma frequently recruits fetal derived cells during pregnancy"</p><p>http://breast-cancer-research.com/content/10/1/R14</p><p>Breast Cancer Research : BCR 2008;10(1):R14-R14.</p><p>Published online 13 Feb 2008</p><p>PMCID:PMC2374970.</p><p></p